TH32757B - A new type of phospholipase A1, its preparation and application process. - Google Patents

Abstract

Phospholipase A1 (phospholipase A1)11 can hydrolyze phospholipids. (phospholipid) to make 2-acyl lysophospholipids (2-acyl lysophospholipid) and can be derived from various Aspergillus species.

Claims (9)

1. Methods for preparing phospholipase A1 (phospholipase A1) including (a) cultivar of (Aspergillus oryzae SANK 11870 FERM BP-3887 under the conditions of producing phospholipase). A1 obtained (b) after allocation. Dilution of the substrate with water or buffer strand (c) filtration of the resulting solution under pressure conditions to separate the non-soluble fraction and (d) may opt to purify the enzyme. 2. Method for preparing phospholiphase A1, which included (a) cultivar of (Aspergillus niger American Type Culture Collection, strain No. 9642, under conditions where phospholiphase A1 was established (b). After breeding Dilution of the substrate with water or buffer strands (c) filtration of the resulting solution under pressure conditions to separate the non-soluble fraction and (d) may choose to purify the enzyme. 3. Method of claim 1 where the strains are reared at a temperature between 18 ° and 40 ° C 4. Method of claim 1 where the strains are fed at temperatures between 18 ° and 32 ° C. 5. Method of claim 1 where the breed is raised between 3 and 20 days 6. Method of claim 1 where the breed is raised between 4 and 8 days 7. The strain was fed at 30 ํ for 15 h and continued at 19 ํ for 5 days. 8. Method of claim 1 where the substrate was diluted by 1 to 20 times by weight of food. In aqueous or actuase buffer solution 9. Method of claim 1 where phospholipase A1 was purified by either method. Or use a combination of methods selected from the containing group; Hair dyeing Column Chromatography - Salting - Out (salting - out) and Gel Filtration 1 0. Method of claim 1 where in step (b) Dilute the culture with buffer solution. Selected from the group containing avatate buffers And buffer solution of phosphate 1 1. Method of claim 4, where the breed is raised for 4 to 8 days 1 2. Method of claim 2 where the breed is raised at a temperature of between 10 ํ 40 ํ C 1. 3. Method of claim 2, where the breed is kept between 18 ํ and 32 ํ C 1. 4. Method of claim 2, where the breed is kept between 3 and 20 days 1 5. Method of claim 2, where the breed is kept between 4 and 8 days 1 6. Method of claim 2, where the breed was raised at 30 ํ for 15 hours and continued at 19 ํ for 5 days 1. 7. Method of claim 2 where the culture is diluted by 1 to 20 times by weight of food. In aqueous or aqueous buffer solution 1 8. Method of claim 2 whereby the phospholiphase A1 has been purified by some method. Or a combination of a selected method from a group that contains Dilating mixtures Column Chromatography-Graphi, Salt-Out-and-Gel Filtration 1 9. Method of claim 2 wherein step (b) dilute the culture with buffer solution. Selected from the group that contains the contents of the acetate buffer And phosphate buffer solution 20 method of claim 13, where strains were fed for 4 to 8 days.