TH26158U - A method for preparing extracts from wild cone fruits with antibacterial activity. - Google Patents

Abstract

OCR 09WP 23/04/2568 The preparation method for antibacterial extracts from the fruit of the *Cyperus rotundus* (a traditional Thai fruit called "wild fruit") is as follows: The fruit is washed, dried, and oven-dried at 50°C for 7 days or until completely dry. It is then pounded in a mortar and pestle and further ground finely using a grinder. The extract is then obtained through fractional extraction using five solvents: dichloromethane, acetone, methanol, ethanol, and water, in that order. The solvents are then evaporated using a rotary evaporator, yielding five fractions of extract. Each fraction is then tested for antibacterial activity against *Staphylococcus aureus*. The results show that the extracts exhibit inhibitory activity against *Staphylococcus aureus* and can be used as key ingredients in various products or as primary raw materials to inhibit bacterial growth and replace chemical inhibitors.

Claims (1)

OCR 10KL (30/05/2568) 1. The preparation method for the antibacterial extract from the fruit of the *Cynanchum* plant is as follows: a. Wash the *Cynanchum* fruits with clean water, then air dry them. Dry them in an oven at 50°C for 7 days, or until completely dry. Crush the dried fruits in a mortar and pestle and then grind them finely using a grinder, obtaining fruit fragments 2-3 mm in size. Extract the crushed fruit fragments using fractional solvent extraction. Immerse the crushed fruit fragments in solvent type 1 (dichloromethane) at a ratio of crushed fruit to solvent of 1:2 for 7 days. Then, filter using a 0.30 micrometer pore mesh to separate the solids from the solution. Set aside the solution (part 1; F1). b. Immerse the separated solids in solvent type 2 (acetone) at a ratio of 1:2 for 7 days. Then, filter using a 0.30 micrometer pore mesh to separate the solids from the solution. A. Immerse the separated solids in solvent type 3, methanol, at a ratio of 1:2 for 7 days. Then filter with a 0.30 micrometer pore size filter to separate the solids from the solution. Set aside (Part 2; F2). B. Immerse the separated solids in solvent type 3, methanol, at a ratio of 1:2 for 7 days. Then filter with a 0.30 micrometer pore size filter to separate the solids from the solution. Set aside (Part 3; F3). C. Immerse the separated solids in solvent type 4, ethanol, at a ratio of 1:2 for 7 days. Then filter with a 0.30 micrometer pore size filter to separate the solids from the solution. Set aside (Part 4; F4). D. Immerse the separated solids in solvent type 5, water, at a ratio of 1:2 for 7 days. Then filter with a 0.30 micrometer pore size filter to separate the solids from the solution. Separate and set aside the solution (part 5; F5). Discard the separated residue. Take all five solutions (F1, F2, F3, F4, and F5) and evaporate the solvent using a rotary evaporator. This will yield five different extracts. Separate the extracts into bottles for testing their antibacterial activity against Staphylococcus aureus.