TH21903Y - A method for extracting high concentrations of saponins from black leeches. - Google Patents

Abstract

------03/03/2020------(OCR) Page 1 of 1 Summary of Invention This invention concerns a method for preparing high-concentration saponins from black sea cucumbers. The steps are as follows: a. The body wall and Cuvier tubules are separated from the black sea cucumber and reduced in size. b. The body wall or Cuvier tubules are dried. c. The dried body wall or Cuvier tubules are then used to extract saponins using a reflux method with 70% ethanol, resulting in a concentrated aqueous extract. d. The concentrated aqueous extract is then partitioned in a solvent to remove low-polarity substances, yielding a crude extract with a high concentration of saponins. ------------ Page 1 of 1 Summary of Invention This invention concerns... The preparation method for high-concentration saponins from black sea cucumbers, which have been shown to lower blood lipid levels and blood pressure, involves extracting the substances from the body wall and cuvier tubules of the black sea cucumber. The steps are as follows: a. The body wall and cuvier tubules are separated from the black sea cucumber and reduced in size. b. The body wall and cuvier tubules are dried. c. The dried body wall and cuvier tubules are then used to extract saponins using the reflux method with 70% ethanol, resulting in a concentrated aqueous extract. d. The low-polarity compounds from the concentrated aqueous extract are then partitioned in a solvent to remove them, yielding a crude extract with a high concentration of saponins.

Claims (1)

------03/03/2563------(OCR) Page 1 of 1 page. Claims 1. A method for preparing high-concentration saponins from black sea cucumbers with the following steps: a. The body wall and cuvier ducts are separated from the black sea cucumber and reduced in size. b. The body wall or cuvier ducts, or a combination thereof, are dried using a selected method from vacuum freeze-drying technology, freeze-drying technology, hot air drying technology, infrared drying technology, solar drying technology, or osmotic dehydration, either individually or in combination. c. The dried body wall or cuvier ducts, or a combination thereof, are then processed. Saponins were extracted using the reflux method with 70% ethanol for 5 rounds. The resulting extracts were combined, and the ethanol was evaporated under low pressure using a rotary evaporator to obtain a concentrated aqueous extract. The low-polarity compounds from this concentrated aqueous extract were then partitioned in different solvents: dichloromethane (CH2Cl2) and ethyl acetate (EtOAc). The solutions remaining in the aqueous layer were further partitioned using n-butanol (n-BuOH). n-butanol (n-BuOH), after separation and evaporation to obtain a crude extract with a high concentration of saponins. ------------ Page 1 of 1. Claims 1. A method for preparing high-concentration saponins from black sea cucumbers, which have the effect of reducing blood lipid levels and blood pressure, by extracting the substances from the body wall and cuvier ducts of black sea cucumbers. The steps are as follows: a. The body wall and cuvier ducts are separated from the black sea cucumber body and reduced in size. b. The body wall or cuvier ducts, or a combination of more than one section, are dried using a method of choice from vacuum freeze-drying technology, freeze-drying technology, hot air drying technology, infrared drying technology, or other drying methods. Drying can be done using solar drying technology or osmotic dehydration, either individually or in combination. A portion or a combination of these dried parts of the body wall or cuvier tubules is then subjected to saponin extraction using reflux with 70% ethanol for five cycles. The resulting extracts are combined, and the ethanol is evaporated under controlled conditions. A concentrated aqueous extract was obtained by using a rotary evaporator. The low-polarity compounds were then separated by partitioning in different solvents: dichloromethane (CH2Cl2) and ethyl acetate (EtOAc). The aqueous solution was then further partitioned using n-butanol (n-BuOH). The n-butanol solution was then evaporated to obtain a crude extract with a high concentration of saponins.