TH19220C3 - Method for preparing phenolic extract from Kluea leaves - Google Patents

Abstract

Summary of the invention which will appear on the advertisement page ReadFile:------23/04/2021------(OCR) The method of preparation of phenolic extract from Klu leaves was taken. Kluaw leaves are washed, dried and ground thoroughly, then extracted by boiling method using distilled water and then filtered. Butanol by using a funnel to separate the butanol layer extract to evaporate dry and dissolve it again with distilled water and adjust the pH (pH) to a value of 1-2, then bring the solution. Next, extract with ethyl acetate (Ethylacetate) and evaporate the ethyl acetate layer of solution to obtain a phenolic extract from the leaves of Klua that looks like a brown powder. Yellow can be used as an ingredient in cosmetic products, food supplements and medicines-------------- Page 1 of 1 page invention summary The method of preparation of high-volume phenolic extract from kluo leaves is washed, dried and ground kluo leaves, then extracted by boiling method using distilled water and filtered out kluo leaf residue. Dichloromethane (Dichloromethane) and Butanol were extracted by using a funnel to separate the extracted butanol layer (Butanol) to evaporate, dry and dissolve it again with distilled water. Adjust the pH (pH) to a value of 1-2, then bring the solution to extract with ethyl acetate (Ethylacetate) and bring the solution of ethyl acetate (Ethylacetate) to evaporate dry to get feed extract. Phenolic, a high content of klua leaves that looks like a yellowish brown powder, can be used as an ingredient in cosmetic products, dietary supplements and medicines.

Claims (2)

Claims (all) which will not appear on the classifieds page:------23/04/2021------(OCR) 1.Phenolic extract preparation process. ) From the kluo leaves which consist of the following steps: a. Select only the tops of the leaves and the young leaves that are complete without lesions or torn, washed thoroughly with distilled water, drenched and dried at a temperature of 50- 55 degrees Celsius bene for 12-18 hours, then take the dried kluo leaves to grind into powder, then sift the kluo leaves powder through a sieve with a hole size equal to 0.5 mm. and store the kluo leaf powder at a temperature of 2-5. B. The extracted kluo leaf powder obtained from the terms was extracted by boiling method using distilled water as a solvent at the extraction ratio of 1:10 (by weight per volume), which was 10 g of kluo leaf powder, followed by 100 ml volume of distilled water is heated at a temperature of 75-85 degrees Celsius, boiled for 10-15 minutes, rested to cool, then filtered with filter paper with a hole size of 11 microns, then take the residue for extraction. Method of boiling with distilled water again and then evaporating the clear liquid by rotary evaporator at 45-50 degrees Celsius to obtain a brown semi-solid, semi-liquid extract. C. Take the crude extract obtained from K. to extract again by using a separation funnel in which the crude extract is dissolved with distilled water in a ratio of 1:20 (by weight per volume). Coarse extraction 1 g per 20 ml of distilled water dissolve the substance well, then add 100 ml of dichloromethane solvent, shake the separation funnel for 5 minutes, set aside to separate the substance and discard. For dichloromethane, repeat 4-5 times until the dichloromethane step is clear, then change the solvent to butanol by adding butanol. ) into the water layer 100 ml each time, shake the separation funnel for 5 minutes, set aside to allow the substance to separate and collect the butanol (Butanol), repeat 4-5 times to obtain a final extract. Butanol D. The extract of butanol (Butanol) obtained from the text is evaporated by a rotary evaporator at a temperature of 42-48 degrees Celsius and then extracted. Separated again by dissolving butanol extract with distilled water at a ratio of 1 g of butanol extract per 100 ml of water, then add hydrochloric acid ( Hydrochloricacid) or acid The concentration of 6 molar salts lowered the solution to the pH value of 1-2. The solution was then filtered with a filter paper with a pore size of 11 microns, and the solution was extracted with a solvent. Ethyl acetate (Ethylacetate) volume 25 ml by using the separation funnel, shaking the separation funnel for 5 minutes, then soaking for 20-30 minutes. in the upper layer, then bring the aqueous solution to extract repeatedly with the solvent ethyl acetate (Ethylacetate), repeat 2-3 times and bring the solution of the ethyl acetate layer (Ethylacetate) to evaporate with the evaporator. Rotary evaporator at a temperature of 42-45 degrees Celsius, which is a phenolic extract that has a powdery appearance, this yellowish-brown color from which the extracted phenolic (Phenolic) has been taken. It can be a component in cosmetic products, food supplements and medicines.-------------- Page 1 of the number 1 claims page. 1. Method for preparing a high-dose phenolic extract from the leaves of kluo, which consists of the following steps: a. Select only the tops of the leaves and the young leaves that are complete, without lesions or tears, and wash them with water. Distilled water and dried and dried at 50-55 degrees Celsius for 12-18 hours. Then, the dried kluo leaves were ground into powder and sifted through a sieve with a hole size equal to 0.5. millimeter and store the extracted kluo leaf powder at a temperature of 2-5 °C. B. The extracted kluo leaf powder was extracted by boiling using distilled water as a solvent at an extraction ratio of 1:10 ( by weight per volume) is 10 g of kluo leaf powder per 100 ml of distilled water, heated at 75-85 degrees Celsius, boiled for 10-15 minutes, rested to cool, and then filtered with filter paper with a hole size of paper 11 microns, then the residue is extracted by boiling with distilled water again, then the clear liquid is evaporated by a rotary evaporator at a temperature of 45-50 degrees Celsius to get a substance. The dark brown semi-solid and semi-solid extract is called crude extract. Dissolution in which the crude extract is dissolved with distilled water at a ratio of 1:20 (by weight per volume) is 1 gram of crude extract per 20 ml of distilled water, dissolved together, then add dichloromethane solvent. Dichloromethane into 100 ml, shake the separation funnel for 5 minutes, set aside for the dichloromethane to separate and discard the dichloromethane part. Repeat 4-5 times until the dichloromethane layer is formed. (Dichloromethane) clear and change the solvent to butanol (Butanol) by adding butanol (Butanol) into the water layer 100 ml each time, shaking the separation funnel for 5 minutes, set aside to separate the substance. Separated and collecting the butanol (Butanol), repeat 4-5 times to get the extract of the butanol layer (Butanol). The text was evaporated with a rotary evaporator at a temperature of 42-48 degrees Celsius and then extracted again by dissolving the butanol layer with distilled water at a ratio of 1. g of butanol extract per 100 ml of water, then add hydrochloric acid (Hydrochloricacid) or saline with a concentration of 6 molar to make the solution have a pH (pH) ) is 1-2 then Filter the solution with filter paper with a paper pore size of 11 microns, then extract the solution with 25 ml of ethyl acetate solvent by using a separation funnel, shaking the separation funnel for 5 minutes and then soaking. for 20-30 minutes, then extract the solution from the upper ethyl acetate layer (Ethylacetate) and bring the aqueous solution to extract again with ethyl acetate (Ethylacetate) solution. 2-3 times and the solution of ethyl acetate layer (Ethylacetate) was evaporated by a rotary evaporator at a temperature of 42-45 degrees Celsius, which yielded a high-volume phenolic extract. It looks like a yellowish brown powder. 2. Method of preparation of high-volume phenolic extract from Klu leaves according to claim 1, where Phenolic extract is used as a component in cosmetic products, food supplements and can cure the disease