TH1901000586A - Methods and components of gene expression in plants. - Google Patents

Abstract

Page 1 of the number 1 page, a summary of the invention. This invention provides a useful hybrid DNA molecule for organizing. Efficient expression of proteins in genetically modified plants including components and How to use hybrid DNA molecules in a specific way The fabrication provides for a DNA molecule to be mixed, and its construction consists of a transport peptide encoding sequence and an actively connected sequence. possible with the transmission of herbicide resistance

Claims (37)

Page 1 of 6 pages Claims 1. A hybrid DNA molecule composed of a DNA sequence encoding a connected transport peptide. This works for DNA sequences encoded for heterologous herbicide resistant proteins, where the transport peptide is composed of amino acid sequences composed of At least 97% identical to the sequence selected from the group consisting of SEQ ID NO: 4-49 and SEQ ID NO: 236-266. 2. A hybrid DNA molecule according to claim 1, where herbicide-tolerant proteins The heterologous form has the activity of protoporphyrinogen oxidase that is not responsive to herbicides. 3. DNA molecules are fused according to claim 1, where herbicide resistant proteins Heterologous composites consist of amino acid sequences consisting of at least 97% identical to that selected from the group containing SEQ ID NO:100-119, SEQ ID NO:163-. 182 and SEQ ID NO:224-228 4. Hybrid DNA molecule according to claim 1, where the peptide-encoded DNA sequence is transported. The composite consists of a composite nucleic acid sequence with at least 97% similarity to the sequence selected from a group consisting of SEQ ID NO:54-99 and SEQ ID NO:267-297. 5. Hybrid DNA molecule according to claim 1, where the DNA sequence that encodes proteins with Resistant to heterologous herbicides consisting of a composite nucleic acid sequence. Combined with at least 97% similarity to the sequence selected from the group consisting of SEQ ID NO:121- 162 and SEQ ID NO: 183-223, SEQ ID NO:229-235. 6. Claim 1 hybrid DNA molecule supplemented with promoter heather. Actively connected rologus with DNA sequences encoding transport peptides. 7. Creation of DNA that is composed of DNA molecules according to Claim 1 that is connected using work with promoter heterologus page 2 of number 6 pages 8. Generating DNA according to claim 7, where the herbicide-tolerant proteins Heterologous has the activity of protoporphyrinogens. Oxidases that do not respond to herbicides 9. Generating DNA according to claim 7, where the herbicide-tolerant proteins Heterologous is composed of amino acid sequences selected from the group consisting of SEQ ID NO: 100-119, SEQ ID NO:163-182 and SEQ ID NO:224-228. 10. Generating DNA according to Claim 7, where DNA generation exists in the genome of a plant, seed, or genetically engineered cell. 11. Genetically modified plants, seeds or cells composed of hybrid DNA molecules under Claim 1. 12. Genetically modified plants, seeds or cells pursuant to Claim 11 where the plant, seed or cell is resistant to one or more PPO herbicides. 13. Plants, seeds or cells genetically modified pursuant to Claim 12 where PPO herbicides are selected from a group that includes: acefluorphen, fomesafen, lactophan, fluoroglycofen-ethyl, oxyfluorene, flumioxacin, azaphenidin, carfenthrazone-ethyl, sulfantrazone, f Luthiacet-methyl, oxadiargil, oxadiazon, pyraflufen-ethyl, saflufenacil, and S-3100. 14. Genetically modified plants, seeds or cells pursuant to Claim 11 where genetically engineered plants, seeds or cells are resistant to at least a second chemical herbicide. 15. Recombinant proteins that are composed of In a functional connection: a) constituent transport peptides with amino acid constituent sequences that are at least 95% identical to that selected from the constituent groups SEQ ID NO:4- 49 and SEQ ID NO.-236- 266 and b) Heterologous herbicide tolerant proteins page 3 of 6 pages. 16. Recombinant proteins according to claim 15, where herbicide-tolerant proteins Heterologous forms have the activity of protoporphyrinogens. Oxidases that do not respond to herbicides 17. Genetically modified plants, seeds or cells composed of recombinant proteins. According to claim 15 18. A method for producing herbicide tolerant plants that includes the steps of: a) molecular DNA conversion of plant cells. Hybrids under claim 1 and b) regeneration of herbicide-tolerant plants composed of DNA molecules. 19. Claim 18 method that additionally combines with the step of germination cross-pollination. New to itself or to a second plant to produce one or more flowering plants. 20. Claim method 18, where a heterologous herbicide tolerant protein is composed of an amino acid sequence selected from a group consisting of SEQ ID NO:100-. 119, SEQ ID NO:163-182 and SEQ ID NO:224-228 21. The method of claim 19, which additionally combines with the procedure for the selection of fruit-bearing plants. Resistance to one or more PPO herbicides. 22. Claim 20 method, where PPO herbicides are selected from a group that includes: ace fluorphan, fomesafen, lactophan, fluoroglycophe. N-ethyl, oxyfluorene, flumioxacin, azaphenidin, carfenthrazone-ethyl, sulfentrazone, fluthiacet-methyl , oxadiar gil, oxadiazon, pyraflufen-ethyl, saflufenacil and S-3100 23. Methods for producing genetically modified plants or seeds that are resistant to herbicides that It combines by cross-pollinating a plant that combines a hybrid DNA molecule, according to claim 1, with itself or a second plant to produce herbicide-tolerant genetically engineered plants or seeds, page 4 of number 6. page 24. Heterologous herbicide tolerant protein expression method in plants. or cellular inclusion methods by growing plants or cells composed of hybrid DNA molecules according to claim 1, where such growth results in the expression of resistant proteins. Against heterologous herbicides 25. Method according to claim 24, where proteins that are resistant to heterogeneous herbicides Rologus has the activity of protoporphyrinogens. Oxidases that do not respond to herbicides 26. Methods for controlling or preventing the growth of weeds in plant growth areas Combined with the use of at least one effective dose of the PPO herbicide. with plant growth areas consisting of genetically modified plants or seeds pursuant to Article 12 where genetically modified plants or seeds are resistant to PPO herbicides. 27. Method according to claim 26, where PPO herbicides are selected from a group that includes: acefluorphen, fomesafen, lactophan, fluoroglycophe. N-Ethyl, Oxyfluphene, Flumioxacin, Achaphenidin, Carfenthrazone-Ethyl, Sulfentrazone, Fluthiacet-Methyl , oxadiar gil, oxadiazon, pyraflufen-ethyl, saflufenacil and S-3100 28. Weed growth control methods that are resistant to herbicides include: a) cultivation in plant or seed growth areas according to Claim 12 and b) application of pesticides. PPO weeds and other herbicides at least one species per area Grow plants where the plant or seed is resistant to PPO herbicides and one or more other herbicides. 29. Claim 28 method, where PPO herbicide tolerance is selected from the Contains acefluorphan, fomezafen, lactophan, fluoroglycofen-ethyl, oxyfluorene, flumioxacin, a Saphenidin, carfenthrazone-ethyl, sulfentrazone, flutiacet-methyl, oxadiarkil, oxadiazon, pyraflufen -Ethyl, saflufenacil and S-3100 30. Claim method 28, where one or more other herbicides are selected from a group that includes: ACCase inhibitor, ALS inhibitor, EPSPS inhibitor, synthetic auxin, inhibitor. Page 5 of a number. 6 pages Photosynthesis, Glutamine Syntetase Inhibitors, HPPD Inhibitors, PPO Inhibitors and Inhibitors long-chain fatty acids 31. Claim 30 method where the ACCase inhibitor is erylloxyphenoxy propionate or cyclohexendione; ALS inhibitors are sulfonylureas, imidazolinone, triazolopyrimidine, or triazolinone; EPSPS inhibitors are glyphosate; The synthetic auxins are phenoxy herbicides, benzoic acid, carboxylic acid, or semicarbazone; Inhibitors of photosynthesis are triacin, triacinone, nitrile, benzothiadiazole, or urea; The glutamine cintetase inhibitor is lufosinate; HPPD inhibitors are isozazole, pyrazolone or triketones; PPO inhibitors are diphenyl ether, N-phenylptalimide, erylltriacinone or pyrimidine dione, or long-chain fatty acid inhibitors are: chloroacetamide, oxyacetamide, or pyrazol 32. A hybrid DNA molecule composed of a DNA sequence encoding a connected transport peptide. It works with DNA sequences encoding proteins that are resistant to heterogeneous herbicides. The transport peptide rologus consists of an amino acid sequence composed of at least 95% affinity to the sequence selected from the constituent group SEQ ID NO:236-266. 33. A hybrid DNA molecule according to claim 32, where proteins that are resistant to elimination agents Heterologous weeds have protoporphyrinogen activity. Oxidases that do not respond to herbicides 34. A hybrid DNA molecule according to claim 32, where proteins that are resistant to elimination agents Heterologous weeds consist of an amino acid sequence consisting of at least 95% homogeneous aggregate to a sequence selected from a group containing SEQ ID NO:100-119, SEQ ID NO:163. - 182 and SEQ ID NO:224-228 35. Hybrid DNA molecule according to claim 32, where the peptide-encoded DNA sequence is transported. The composite consists of a composite nucleic acid sequence with at least 95% affinity to the sequence selected from the composed group SEQ ID NO:267-297. 36. Hybrid DNA molecule according to claim 32, where the DNA sequence encoding proteins Resistant to heterologous herbicides. Consists of composite sequences of nucleic acids, page 6 of 6 pages, totaling at least 95% similarity to sequences selected from groups consisting of SEQ ID NO:121- 162 and SEQ ID NO:183-223, SEQ ID NO:229-235 37. Hybrid DNA molecule pursuant to Claim 32 supplemented with promoter heather. Actively connected rologus with DNA sequences encoding transport peptides.