TH18772Y - A process for enabling nitrogen-fixing cyanobacteria to coexist with plant roots. - Google Patents

Abstract

Page 1 of 1. Summary of the Invention. This invention concerns a method for symbiotically colonizing plant roots with nitrogen-fixing cyanobacteria. This is achieved by soaking root sections of plant seedlings in BG-11No medium containing short rods of nitrogen-fixing cyanobacteria, or by soaking root sections of plant seedlings that have been exposed to high-frequency waves of 50/60 Hz for 30 seconds in BG-11N0 medium containing short rods of nitrogen-fixing cyanobacteria for 5 days. The result is plant seedlings with cyanobacteria attached to their roots. When these seedlings are used as planting material, they exhibit better growth and higher yields. This invention provides another approach to applying nitrogen-fixing cyanobacteria as a biofertilizer. This application requires a smaller quantity of cyanobacteria compared to directly applying biofertilizer to the cultivated area. Furthermore, the cyanobacteria... The nitrogen-fixing nobacteria, as described in this invention, that coexist with plant roots can immediately begin the nitrogen fixation process, thus reducing the time and complexity of the process. The use of such nitrogen-fixing nobacteria also results in increased plant growth and yield, making it an effective method.

Claims (7)

Page 1 of 2. Claims. 1. The process of enabling nitrogen-fixing cyanobacteria to coexist with plant roots consists of: The steps are as follows: a. Cultivate cyanobacteria in BG-11No medium to increase their population. Increased until there was a sufficient quantity for operations. B. Harvest the cyanobacteria from step A by crushing them into pieces. In short, using a tissue grinder, you will get nitrogen-fixing cyanobacteria that have a specific structure. Short section C. Add the nitrogen-fixing cyanobacteria that have been processed in step B to the formulated medium. BG-11No. again. d. Soak the root portions of the plant seedlings in BG-11No medium containing capable cyanobacteria. Nitrogen fixation according to step C for 5 days will result in plant seedlings with cyanobacteria attached to the area. Roots of a seedling 2. A method for enabling nitrogen-fixing cyanobacteria to coexist with plant roots as per claim 1. Where the cultivation of cyanobacteria in BG-11N0 medium involves culturing short, crushed pieces of bacteria. Grind 8 milliliters of cyanobacteria tissue and place it in a 1,500 milliliter plastic bottle containing... Inoculate 1,000 ml of BG-11No medium until the cyanobacteria multiply to a sufficient quantity. Usage 3. A method for enabling nitrogen-fixing cyanobacteria to coexist with plant roots as per claim 1. Or, in two ways, cyanobacteria are harvested by taking cyanobacteria that have been cultured in plastic bottles. A 1,500 ml sample was centrifuged at a speed of 10,000 revolutions per second for 15 minutes, then... Discard the clear liquid and keep the cell portion; you will obtain cyanobacteria cells. 4. A method for enabling nitrogen-fixing cyanobacteria to coexist with plant roots as per claim 1. This is where the roots of plant seedlings are located; these are seedlings that have been grown from seeds until they are 7-10 days old. 5. A method for enabling nitrogen-fixing cyanobacteria to coexist with plant roots as per claim 1. Of the four options, the most suitable rootstock for a plant seedling is one that has been grown from a seed. 8-day-old plants 6. A method for enabling nitrogen-fixing cyanobacteria to coexist with plant roots as per claim 1. One of the five options is where the root of the plant seedling is placed in distilled water contained in a beaker. Then, it is exposed to a high-frequency wave of 50/60 Hertz for 30 seconds using an ultrasonic device. (Ultrasonic cleaner) to stimulate the outer surface tissue of the root to open. Page 2 of 2 pages. 7. Method for enabling nitrogen-fixing cyanobacteria to coexist with plant roots as per the claim. Any one of the following, from 1 to 6, is a case where plant seedlings have cyanobacteria attached to their roots for use as... Seedlings for further planting.