TH17879C3 - Directional labeling nanosensor test kit - Google Patents

Abstract

Summary of the invention which will be displayed on the ad page ReadFile:------13/09/2019------(OCR) Page 1 of 1 page. invention summary This invention involves labeling directional nanosensor assays. This assay can reduce the labeling problems associated with conventional assays such as physical or chemical methods. In this study, ligand-specific proteins were selected for the detection of target molecules for use in ligand-labeling to reporters with a directional method that could reduce the problem of ligand detachment from labeling and The problem of degradation of ligand binding efficiency can also save the amount of ligand used and preserve the functional group condition of the ligand from the labeling method. The physical and chemical methods can be sequenced and help determine the direction of the ligand itself in the interaction with the target molecule. Targets with sandwich binding, direct capture, indirect capture and can be applied to the detection of other target molecules such as contaminants or chemical contaminants in food or various pathogens combined. Both other target substances and the detection of target molecules using other techniques as well--------------- ---13/09/2019------(OCR) Page 1 of 1 page number invention summary This invention involves labeling directional nanosensor assays. This assay can reduce the labeling problems associated with conventional assays such as physical or chemical methods. In this study, ligand-specific proteins were selected for the detection of target molecules for use in ligand-labeling to reporters with a directional method that could reduce the problem of ligand detachment from labeling and The problem of degradation of ligand binding efficiency can also save the amount of ligand used and preserve the functional group condition of the ligand from the labeling method. The physical and chemical methods can be sequenced and help determine the direction of the ligand itself in the interaction with the target molecule. Targets with sandwich binding, direct capture, indirect capture and can be applied to the detection of other target molecules such as contaminants or chemical contaminants in food or various pathogens combined. both other target substances and the detection of target molecules using other techniques as well------------ 1 of 1 page invention summary This invention involves labeling directional nanosensor assays. This assay can reduce the labeling problems associated with conventional assays such as physical or chemical methods. In this study, ligand-specific proteins were selected for the detection of target molecules for use in ligand-labeling to reporters with a directional method that could reduce the problem of ligand detachment from labeling and The problem of degradation of ligand binding efficiency can also save the amount of ligand used and preserve the functional group condition of the ligand from the labeling method. The physical and chemical methods can be sequenced and help determine the direction of the ligand itself in the interaction with the target molecule. Targets with sandwich binding, direct capture, indirect capture and can be applied to the detection of other target molecules such as contaminants or chemical contaminants in food or various pathogens combined. both other target substances and the detection of target molecules using other techniques as well.

Claims (9)

Claims (all) which will not appear on the advertisement page:------13/09/2019------(OCR) Page 1 of 2 pages Claim 1: Labeling directional nanosensor detection kit comprising: sample drop pad The ligand-loaded disc The signal display plate contains control lines and test lines. The remaining liquid collection plate that has specific characteristics The ligand-loading plate contains ligand labeled with a directional reporter. The ligand-loading membrane is coated in two layers, with the first layer coated with A buffer solution containing a substance that preserves the reporter and is coated with another layer. with ligand labeled with a directional reporter. A directional reporter-labeled ligand contains a reporter molecule. The reporter is dissolved in a buffer solution containing a substance that preserves the reporter and The reporter is labeled with a molecule that is specific to the FC region of the molecule. Target molecule-specific antibody 2. Directional labeling nanosensor according to claim 1, wherein the sample drop plate Such ligand-loading, signaling, and residual liquid storage membranes are membranes that Choose from glass fiber sheets, cotton fiber sheets, polymer fiber sheets, paper sheets, cellulose sheets, derivative sheets. Cellulose, glass fiber sheets mixed with cotton fibers, glass fiber sheets mixed with polymer fibers, cotton fiber sheets mixed with polymer fibers, which One or more of the 3 directional labeling nanosensors according to claim 1, wherein the buffer solution Which can be selected from phosphate buffer, Tris buffer, carbonate buffer, borate buffer? One or a combination of these and the substance that serves to preserve the reporter can be selected from the protein. Bovine albumin, egg albumin protein, casein, polysorbate, glucose, sucrose, trehalose. One or more of the 4 directional labeling nanosensors according to claim 1, wherein the reporter molecule As such, select from organic dyes, inorganic dyes, enzymes, fluorescent substances, luminescent substances, polymers. Color metal particles, inorganic particles, Raman signals, infrared signals. One or more of the 5 directional labeling nanosensors according to claim 1, wherein the molecule containing The specificity of the FC region of such antibody molecules is selected by the peptide nucleic chain. One or a combination of these protein pna-antibody chains. Page 2 of 2 Page 6 Directional labeling nanosensors according to claim 5, wherein said protein is selected. It is obtained from the molecule of protein A, protein G, protein L, protein C, protein fusion between protein A and Protein GProtein fusion between protein A and protein LProtein fusion between protein A and protein C Fusion protein between G protein and L proteinFusion protein between G protein and C protein Fusion between protein L and protein C. Protein fusion between protein A and protein G and protein L. fusion protein between protein A and protein G and cey protein, fusion protein between protein A and protein L and Sey protein, a fusion protein between G protein and either L protein and Sey protein, or A combination of 7 directional labeling nanosensors according to claim 1, wherein the test line area The signal plate has a molecule that is immobilized on the test line. The molecule can be selected from the molecule. A molecular target that has a similar structure to a ligand target that is specific to the target molecule in what way? One or more of the 8 directional labeling nanosensors according to claim 1, wherein the control line region of the signal plate contains a molecule that is immobilized on the control line. The molecule is a molecule that has Ligand specificity------------------13/09/2019------(OCR) Page 1 of 2 pages Claim 1: Labeling directional nanosensor detection kit comprising: sample drop pad The ligand-loaded disc The signal display plate contains control lines and test lines. The remaining liquid collection plate that has specific characteristics The ligand-loading plate contains ligand labeled with a directional reporter. The ligand-loading membrane is coated in two layers, with the first layer coated with A buffer solution containing a substance that preserves the reporter and is coated with another layer. with ligand labeled with a directional reporter. A directional reporter-labeled ligand contains a reporter molecule. The reporter is dissolved in a buffer solution containing a substance that preserves the reporter and The reporter is labeled with a molecule that is specific to the FC region of the molecule. Target molecule-specific antibody 2. Directional labeling nanosensor according to claim 1, wherein the sample drop plate Such ligand-loading, signaling, and residual liquid storage membranes are membranes that Choose from glass fiber sheets, cotton fiber sheets, polymer fiber sheets, paper sheets, cellulose sheets, derivative sheets. Cellulose, glass fiber sheets mixed with cotton fibers, glass fiber sheets mixed with polymer fibers, cotton fiber sheets mixed with polymer fibers, which One or more of the 3 directional labeling nanosensors according to claim 1, wherein the buffer solution Which can be selected from phosphate buffer, Tris buffer, carbonate buffer, borate buffer? One or a combination of these and the substance that serves to preserve the reporter can be selected from the protein. Bovine albumin, egg albumin protein, casein, polysorbate, glucose, sucrose, trehalose. One or more of the 4 directional labeling nanosensors according to claim 1, wherein the reporter molecule As such, select from organic dyes, inorganic dyes, enzymes, fluorescent substances, luminescent substances, polymers. Color metal particles, inorganic particles, Raman signals, infrared signals. One or more of the 5 directional labeling nanosensors according to claim 1, wherein the molecule containing The specificity of the FC region of such antibody molecules is selected by the peptide nucleic chain. One or a combination of these protein pna-antibody chains. Page 2 of 2 Page 6 Directional labeling nanosensors according to claim 5, wherein said protein is selected. It is obtained from the molecule of protein A, protein G, protein L, protein C, protein fusion between protein A and Protein GProtein fusion between protein A and protein LProtein fusion between protein A and protein C Fusion protein between G protein and L proteinFusion protein between G protein and C protein Fusion between protein L and protein C. Protein fusion between protein A and protein G and protein L. fusion protein between protein A and protein G and cey protein, fusion protein between protein A and protein L and Sey protein, a fusion protein between G protein and either L protein and Sey protein, or A combination of 7 directional labeling nanosensors according to claim 1, wherein the test line area The signal plate has a molecule that is immobilized on the test line. The molecule can be selected from the molecule. A molecular target that has a similar structure to a ligand target that is specific to the target molecule in what way? One or more of the 8 directional labeling nanosensors according to claim 1, wherein the control line region of the signal plate contains a molecule that is immobilized on the control line. The molecule is a molecule that has Ligand specificity------------Revised 15/02/2019 No claims---------- Page 1 of 2 pages Claims 1. Directional labeling nanosensor detection kit consists of A. sample drop plate B. Ligand-loaded disc C. The signal display plate contains control lines and test lines. D. Remaining liquid collection plate What is unique is that the ligand-loaded patch is labeled with a directional reporter. 2. Directional labeling nanosensor as per claim 1, wherein said sample drop plate, ligand loading plate, signal display plate, and said residual liquid collection plate are selectable membranes. From glass fiber sheets, cotton fiber sheets, polymer fiber sheets, paper sheets, cellulose sheets, cellulose derivative sheets, glass fiber sheets, cotton fiber sheets, glass fiber sheets, polymer fiber sheets, cotton fiber sheets, polymer fiber sheets. one or a combination of these 3. The directional labeling nanosensor according to claim 2, wherein said ligand loading membrane is coated in two steps, first with a buffer solution containing the substance. that retains the reporter and is coated with a layer of directional reporter-labeled ligand. 4. Directional labeling nanosensor according to claim 3, wherein said buffer solution is selected from one or more phosphate buffers, trise buffers, carbonate buffers, borate buffers. combined and the reporter preservation agent can be selected from one or more of bovine albumin, egg albumin, casein, polysorbate, glucose, sucrose, trehalose. together 5. Directional labeling nanosensor kit according to claim 3, wherein the directional reporter-labeled sigand consists of a reporter molecule dissolved in a buffer solution containing the substance. It maintains the reporter and the reporter is labeled with a molecule that is specific to the FC region of the antibody molecule that is specific to the target molecule. 6. Directional labeling nanosensor as per claim 5, wherein said reporter molecule is selectable from an organic dye, an inorganic dye, an enzyme, a fluorescent agent, a luminase substance. Polymer Sense Page 2 of 2 pages gives color to metal particles, inorganic particles, Raman signals, infrared signals, one or a combination of them. 7. Directional labeling nanosensor according to claim 5, wherein the FC region-specific molecule of said antibody molecule is selected from the peptide nucleic chain. One or a combination of these protein pna-antibody chains. 8. Directional labeling nanosensor according to claim 5, wherein said protein is selected from protein A, protein G, protein L, protein C protein, fusion between protein A and protein G. Fusion protein between protein A and protein L. Protein fusion between protein A and protein C. Protein fusion between protein G and protein L. Fusion protein between protein G and protein. Sey protein fusion between protein L and protein Sey protein fusion between protein A and protein G and protein L protein fusion between protein A and protein G and Sey protein fusion protein between protein A and protein L and protein Cey protein fusion between protein G and protein L and one or a combination of Cey protein 9. The directional labeling nanosensor according to claim 1, wherein the test line region of the signal plate contains a molecule immobilized on the test line, said molecule selected from the available target molecules. A ligand target-like structure that is specific to one or a combination of target molecules1. 0. The directional labeling nanosensor according to claim 1, wherein the control line region of the signal plate has a molecule immobilized on the control line, said molecule being a ligand-specific molecule. Old