TH17879A3 - Directional labeling nanosensor test kit - Google Patents

Abstract

Summary of the invention which will be displayed on the ad page ReadFile:------13/09/2019------(OCR) Page 1 of 1 page. invention summary This invention involves labeling directional nanosensor assays. This assay can reduce the labeling problems associated with conventional assays such as physical or chemical methods. In this study, ligand-specific proteins were selected for the detection of target molecules for use in ligand-labeling to reporters with a directional method that could reduce the problem of ligand detachment from labeling and The problem of degradation of ligand binding efficiency can also save the amount of ligand used and preserve the functional group condition of the ligand from the labeling method. The physical and chemical methods can be sequenced and help determine the direction of the ligand itself in the interaction with the target molecule. Targets with sandwich binding, direct capture, indirect capture and can be applied to the detection of other target molecules such as contaminants or chemical contaminants in food or various pathogens combined. Both other target substances and the detection of target molecules using other techniques as well--------------- ---13/09/2019------(OCR) Page 1 of 1 page number invention summary This invention involves labeling directional nanosensor assays. This assay can reduce the labeling problems associated with conventional assays such as physical or chemical methods. In this study, ligand-specific proteins were selected for the detection of target molecules for use in ligand-labeling to reporters with a directional method that could reduce the problem of ligand detachment from labeling and The problem of degradation of ligand binding efficiency can also save the amount of ligand used and preserve the functional group condition of the ligand from the labeling method. The physical and chemical methods can be sequenced and help determine the direction of the ligand itself in the interaction with the target molecule. Targets with sandwich binding, direct capture, indirect capture and can be applied to the detection of other target molecules such as contaminants or chemical contaminants in food or various pathogens combined. both other target substances and the detection of target molecules using other techniques as well------------ 1 of 1 page invention summary This invention involves labeling directional nanosensor assays. This assay can reduce the labeling problems associated with conventional assays such as physical or chemical methods. In this study, ligand-specific proteins were selected for the detection of target molecules for use in ligand-labeling to reporters with a directional method that could reduce the problem of ligand detachment from labeling and The problem of degradation of ligand binding efficiency can also save the amount of ligand used and preserve the functional group condition of the ligand from the labeling method. The physical and chemical methods can be sequenced and help determine the direction of the ligand itself in the interaction with the target molecule. Targets with sandwich binding, direct capture, indirect capture and can be applied to the detection of other target molecules such as contaminants or chemical contaminants in food or various pathogens combined. both other target substances and the detection of target molecules using other techniques as well.

Claims (9)

1. Claims (all) which will not appear on the advertisement page:------13/09/2019------(OCR) Page 1 of the total of 2 pages. Claim 1. The labeling directional nanosensor assay consists of a. Sample drop sheet b. Ligand-containing plates c. The signal pad consists of a control line and a test line. The rest of the liquid collection plate. That is unique. The ligand loading plate contained a ligand that was labeled with a directed reporter. The ligand-containing membrane was coated in two layers, the first layer being coated with A buffer solution containing a substance that retains the reporter and is coated over it. with a ligand labeled with a directed reporter A ligand labeled with a directed reporter contains reporter molecules. The reporter was dissolved in a buffer solution containing the retaining agent and the reporter. Such reporters are labeled with molecules that are specific to the Fc region of the molecule. antibody specific to the target molecule 2. Directed labeling nanosensor assay in claim 1 where the sample dropper plate The ligand-containing plate, the signal plate and the rest of the fluid collection plate are the membranes that Choose from glass fiber mat, cotton fiber mat, polymer fiber mat, paper sheet, cellulose sheet, derivative sheet of cellulose fiberglass mat mixed with cotton mat fiberglass mat mixed with polymer fiber cotton mat mixed with polymer fiber somehow 3. Directed labeling nanosensor assays in Claim 1 where the buffer solution It can be selected from phosphate buffer, Tris buffer, Carbonate buffer, Borate buffer, somehow. One or more combinations and reporter retention agents are selected from proteins. bovine albumin, egg albumin, casein, polysorbate, glucose, sucrose, trehalose, somehow 4. Oriented nanosensor assays in claim 1 labeling where reporter molecules It can be selected from organic pigments, inorganic pigments, enzymes, fluorescent agents, aluminescence polymers. paints, metal particles, inorganic particles, Raman emitters, infrared emitters in any way 5. Oriented nanosensor assays in claim 1 labeling where molecules with The specificity to the FC region of such antibody molecules was selected from the peptide nucleic line. one or a combination of antibody PRNA proteins Page 2 of number 2 Page 6. Directional nanosensor assays in claim 5 labeling where the protein was selected. obtained from molecules of protein A, protein G, protein L, protein C, protein fusion between protein A and Protein G, protein fusion between protein A and protein L, protein fusion between protein A and protein C. fusion protein between G protein and L protein, fusion protein between G protein and cyprotein Fusion between protein L and protein C, fusion between protein A and protein G and protein L. fusion protein between protein A and protein G and protein cyprotein fusion between protein A and protein L and cy proteins fusion between G proteins and either L and cy proteins or 7. Directional nanosensor assay in claim 1 labeling where the test line region of the signal plate contains molecules that are immobilized on the test line and such molecules are selected from the molecules Molecular targets that are structurally similar to ligand targets that are somehow specific to the target molecule. 8. Directional nanosensor assays in claim 1 labeling where the control line region of the signal plate contains molecules that are immobilized on the control line by such molecules as molecules with Specificity to ligands------------------09/13/2019------(OCR) Page 1 of 2 Claim 1. The labeling directional nanosensor assay consists of a. Sample drop sheet b. Ligand-containing plates c. The signal pad consists of a control line and a test line. The rest of the liquid collection plate. That is unique. The ligand loading plate contained a ligand that was labeled with a directed reporter. The ligand-containing membrane was coated in two layers, the first layer being coated with A buffer solution containing a substance that retains the reporter and is coated over it. with a ligand labeled with a directed reporter A ligand labeled with a directed reporter contains reporter molecules. The reporter was dissolved in a buffer solution containing the retaining agent and the reporter. Such reporters are labeled with molecules that are specific to the Fc region of the molecule. antibody specific to the target molecule 2. Directed labeling nanosensor assay in claim 1 where the sample dropper plate The ligand-containing plate, the signal plate and the rest of the fluid collection plate are the membranes that Choose from glass fiber mat, cotton fiber mat, polymer fiber mat, paper sheet, cellulose sheet, derivative sheet of cellulose fiberglass mat mixed with cotton mat fiberglass mat mixed with polymer fiber cotton mat mixed with polymer fiber somehow 3. Directed labeling nanosensor assays in Claim 1 where the buffer solution It can be selected from phosphate buffer, Tris buffer, Carbonate buffer, Borate buffer, somehow. One or more combinations and reporter retention agents are selected from proteins. bovine albumin, egg albumin, casein, polysorbate, glucose, sucrose, trehalose, somehow 4. Oriented nanosensor assays in claim 1 labeling where reporter molecules It can be selected from organic pigments, inorganic pigments, enzymes, fluorescent agents, aluminescence polymers. paints, metal particles, inorganic particles, Raman emitters, infrared emitters in any way 5. Oriented nanosensor assays in claim 1 labeling where molecules with The specificity to the FC region of such antibody molecules was selected from the peptide nucleic line. one or a combination of antibody PRNA proteins Page 2 of the number 2, page 6. Directional nanosensor assays in claim 5 labeling where the protein was chosen. obtained from molecules of protein A, protein G, protein L, protein C, protein fusion between protein A and Protein G, protein fusion between protein A and protein L, protein fusion between protein A and protein C. fusion protein between G protein and L protein, fusion protein between G protein and cyprotein Fusion between protein L and protein C, fusion between protein A and protein G and protein L. fusion protein between protein A and protein G and protein cyprotein fusion between protein A and protein L and cy proteins fusion between G proteins and either L and cy proteins or 7. Directional nanosensor assay in claim 1 labeling where the test line region of the signal plate contains molecules that are immobilized on the test line and such molecules are selected from the molecules Molecular targets that are structurally similar to ligand targets that are somehow specific to the target molecule. 8. Directional nanosensor assays in claim 1 labeling where the control line region of the signal plate contains molecules that are immobilized on the control line by such molecules as molecules with Specificity to ligands------------Revised 15/02/2019No claim----------Page 1 of 2 pages, claim 1.set Directional nanosensor detection in labeling consisting of a. Sample drop sheet b. Ligand-containing plates c. The signal pad consists of a control line and a test line. The rest of the liquid collection plate. Characteristic is that the ligand-containing plate is labeled with a directed reporter. 2. Directional labeling nanosensor assays according to claim 1, in which the sample dropper, ligand-containing plate, signal plate and the residual fluid collection plate are the selective membranes. From Glass Fiber Mat, Cotton Fiber Sheet, Polymer Fiber Sheet, Paper Sheet, Cellulose Derivative Sheet, Fiber Glass-Cotton Sheet, Polymer Fiber-Cotton Sheet, Polymer-Fiber-Cotton Sheet either one or a combination of them 3. Directional labeling nanosensor assays according to claim 2, in which the ligand-containing membrane is coated in two steps, firstly coated with a buffer solution containing the compound. The reporter retains the reporter and is coated with a ligand labeled with the directed reporter. 4. Directional nanosensor assays in claim 3 labeling where such buffer solutions are selected from one or more of the phosphate buffers, tris, carbonate buffers, borate buffers. The combination and the reporter retention agent were selected from bovine albumin, egg albumin, casein, polysorbate, glucose, sucrose, trehalose, either or more. collectively 5. Directed labeling nanosensor assays according to Claim 3, where the directional reporter-labelled cigands consist of reporter molecules dissolved in a buffer solution containing the directional reporter. The reporter retains the reporter and the reporter is labeled with a specific molecule to the Fc region of the antibody molecule that is specific to the target molecule. 6. Directional labeling nanosensor assays according to claim 5 where such reporter molecules are selected from organic dyes, inorganic dyes, enzymes, fluorescence compounds, luminescence. polymer sense Page 2 of a number of 2 pages paints metal particles, inorganic particles, Raman emitters, infrared emitters, one or more of them together. 7. Dig a directional nanosensor in claim 5 labeling where molecules specific to the FC region of the antibody molecule are selected from the peptide chain nucleic acid. one or a combination of antibody PRNA proteins 8. Directed labeling nanosensor assays according to claim 5 where such proteins are selected from the A, G, L, C, protein A and G fusion molecules. fusion protein between protein A and protein fusion protein between protein A and protein fusion protein between protein G and protein fusion protein between protein G and protein cyprotein fusion between proteins L and cyprotein fusion between proteins A and G and cyprotein fusion between proteins A and G and cyprotein fusion proteins. between protein A and protein L and protein C, protein fusion protein G and protein L and protein C, either or a combination of them. 9. Directional labeling nanosensor assays according to claim 1, where the test line region of the transducer contains a molecule immobilized on the test line where the molecule is selected from the structured molecular target molecule. ligand-like targets that are specific to one or more of the target molecules1 0. A directed nanosensor assay in claim 1 where the control line region of the transducer contains a molecule immobilized by the ligand-specific molecule. d