TH16785B - A process for producing a single cell protein of Candida utilis for use as a raw material for animal feed protein. - Google Patents

Abstract

------ 24/01/2019 ------ (OCR) page 1 of number 1 page invention summary. A process for producing a single cell protein of Candida utilis for use as a raw material for animal feed protein. It is the introduction of yeast, Candida utilis strain that can be isolated from the production process in the chicken processing industry. To raise in the raw material (Substrate) left over from the production process that includes The remainder of the pinch from the production of processed chicken products And the remaining water from the steaming process, processed chicken products With water preparation methods before being used as follows The remaining starch from chicken production was processed into glucose by 1-2% enzyme amylase for 3-4 hours and the two types of water were extracted. Centrifuge to separate the sediment. They were then sterilized before being used as a substrate raw material (Substrate) when cultured until the proper amount of cells was obtained. To separate cells by centrifugation (Centrifuge), then wash the cells clean. And the resulting cells dried Then packed into a container. ------------ ------ 22/08/2561 ------ (OCR) Page 1 of the number 1 page Summary of invention A process for producing a single cell protein of Candida utilis for use as a raw material for animal feed protein. It is the separation of yeast strains Candida utilis. From the production process in the chicken meat processing industry To raise in the substrate (Substrate) left over from Production process that includes The remaining flour from the production of processed chicken products. And the remaining water from the steaming process Processed chicken products With water preparation methods before being used as follows The remaining flour from the production of processed chicken products Figure to digest starch into glucose (Glucose) with enzyme amylase (Enzyme Amylase) ratio 1-2% for 3-4 hours and the water of the two types centrifuge (Centrifuge) to separate the sediment. They were then sterilized before being used as a substrate raw material (Substrate) when cultured until the proper amount of cells was obtained. To separate cells by centrifugation (Centrifuge), then wash the cells clean. And the resulting cells dried Then put the container. ------------ Page 1 of the number 1 page Summary of invention Process for producing single cell protein (Single Cell Protein) for use as a raw material. Animal feed protein The yeast was extracted from the Candida utilis strains. Production process in the poultry processing industry To raise in the substrate (Substrate) left over from Production process that includes The remaining flour from the production of processed chicken products. And the rest of the water from Steaming process of processed chicken products With water preparation processes before being used as follows The flour that The residue from the production of chicken meat is processed to digest starch into glucose (Glucose) with a 1-2% enzyme amylase (Enzyme Amylase) ratio for 3-4 hours and centrifuge of both types of water. To separate the sludge And then sterilized before being used as a substrate (Substrate) when cultured until Cells in the right amount To separate cells by centrifuge (Centrifuge), then wash the cells thoroughly and the cells that have been dried. And put it in a container

Claims (1)

Disclaimer (all) which will not appear on the advertisement page. : ------ 24/01/2019 ------ (OCR) Page 1 of 1 page Disclaimer 1. Process for single cell protein production of Candida Uti bacteria. List (Candida utilis) for use as Raw material sources of animal feed protein are as follows: a. Substrate treatment process that contains residual paste from the production of processed chicken products. And the remaining water from the steaming process of processed chicken products By the remaining starch from the production of processed chicken products To be digested into glucose (Glucose) with enzyme amylase (Enzyme Amylase) ratio 1-2% for 3-4 hours, then bring both types of water into centrifuge (Centrifuge) at 3,000- 5,000 rpm for 10-20 minutes, the clear parts of both types of water were sterilized at 121 ° C for 15 minutes while the sludge of both types of water was dried at 70-80 ° C for 2 hours. -3 h. B. A process of preparation for yeast precursors of Candida utilis strain that was isolated from Production process in the poultry processing industry Yeast, Candida utilis, was cultured on a Potato Dextrose Agar (PDA) medium and then incubated at The temperature was 34-36 ° C for 22-26 h, after which Candida utilis was removed from Potato Dextrose Agar (PDA). 1 loop is placed in 100 ml of Yeast Extract Peptone Dextrose Broth (YPD) culture medium and then incubated at 18-27 ° C for time. 22-26 hours with shaking all the time After that, the Candida utilis solution was applied to adjust the concentration. With Yeast Extract Peptone Dextrose Broth (YPD) culture medium at 0.50-0.55 Mechfaland (McFarland) C. Process for single-cell protein production ( Single Cell Protein) by mixing both types of sterilized water. The ratio of residual paste from the production of processed chicken products is 20-40% and the remaining water from the steaming process. 60-80% processed chicken meat, then the starter of yeast, Candida utilis strain 0.50-0.55, McFarland 10-20. % Put into the substrate (Substrate) 80-90%, then cured at temperature 18-27 degrees Celsius for 22-26 hours, shaking all the time. After that, the cells were collected by centrifuge (Centrifuge) at a speed of 3,000-5,000 rpm for 10-20 minutes, washed the cells with clean water 1-2 cycles and dried at 70-80 degrees Celsius for a time. 30-40 minutes and storage of single cell proteins in containers ------------ ------ 22/08/2018 ------ (OCR) Page 1 of Number 1 Page. 1. Process for producing single cell protein (Candida utilis) of Candida utilis for use as Raw materials, protein sources, animal feed are as follows: A. Substrate preparation process that consists of water, starch left over from the production of processed chicken products. And the remaining water from the steaming process of processed chicken products By the remaining starch from the production of processed chicken products Used to digest the starch into glucose (Glucose) with enzyme amylase (Enzyme Amylase) ratio 1-2% for 3-4 hours, then bring both types of water to centrifuge (Centrifuge) at 3,000-5,000 speed. rpm for 10-20 minutes, the clear parts of both types of water were sterilized at 121 degrees Celsius for 15 minutes and the sludge of both types of water was dried at 70-80 degrees Celsius for 2- 3 h. B. Preparation of yeast strains of Candida utilis (Candida utilis) that were isolated from Production process in the poultry processing industry The yeast strains Candida utilis were cultured on the Potato Dextrose Agar (PDA) medium and then incubated at The temperature is 34-36 degrees Celsius for a time. 22-26 hours later, 1 loop of Candida utilis was removed from Potato Dextrose Agar (PDA) medium. 100 ml of Yeast Extract Peptone Dextrose Broth (YPD) culture medium was then incubated at 18-27 ° C for 22-26 hours by shaking all the time. After that, the Candida utilis solution was applied to adjust the concentration. With Yeast Extract Peptone Dextrose Broth (YPD) culture medium at 0.50-0.55 Mechfaland (McFarland) C. Process for single-cell protein production ( Single Cell Protein) by mixing both types of sterilized water. The ratio of water, starch residue from the production of processed chicken products 20-40% and the residual water from the steaming process. 60-80% processed chicken products, then the starter of the Candida utilis yeast strains 0.50-0.55, McFarland 10-20. % Put into the substrate (Substrate) 80-90%, then cured at temperature 18-27 degrees Celsius for 22-26 hours with shaking all the time After that, the cells were collected by centrifuge (Centrifuge) at a speed of 3,000-5,000 rpm for 10-20 minutes, washed the cells with clean water 1-2 cycles and dried at a temperature of 70-80 degrees Celsius for a time. 30-40 minutes and storage of single cell proteins in containers ------------ ------ 15/03/2018 ------ (OCR) Page 1 of Number 1 Page Disclaimer 1. Process for producing single cell protein (Single Cell Protein) for use as raw material. Animal feed protein The steps are as follows: A. Process of preparation of raw materials (Substrate) containing residual starch juice from production. Processed chicken and beef left over from steaming process, processed chicken products By the remaining starch water Produce chicken meat products to digest starch into glucose (Glucose) with 1-2% enzyme amylase (Enzyme Amylase) ratio for 3-4 hours, then bring both types of water to centrifuge (Centrifuge). At a speed of 3.000-5,000 rpm for 10-20 minutes, the clear parts of both types of water were sterilized at a temperature of 121 degrees Celsius for 15 minutes, and the sludge of both types of water was dried at a temperature of 70-80. B. Yeast preparation process of Candida Utilis strains (Ccmdida Utilis) isolated from the production process in the poultry processing industry. The yeast strains Candida Utilis were cultured on the Potato Dextrose Agar (PDA) medium and then incubated at The temperature was 34-36 ° C for 22-26 hours, after which Candida Utilis was disinfected from Potato Dextrose Agar (PDA). 1 loop is placed in 100 ml of Yeast Extract Peptone Dextrose Broth (YPD) medium and then incubated at temperature. 18-27 degrees Celsius for 22-26 hours, shaking all the time. After that bring Candida Utilis solution was concentrated with Yeast Extract Peptone Dextrose Broth (YPD) culture medium at 0.50. -0.55 McFarland (McFarland) C. Process for producing single cell protein (Single Cell Protein) by mixing both types of sterilized water and mixing it with the ratio of starch left over from processed chicken production. 20-40% and the remaining steamed chicken products 60-80%, then the starter of the Candida Utilis strain of yeast (Candida Utilis) at 0.50 concentration. -0.55 McFarland (McFarland) 10-20% is added to Substrate 80-90%, incubated at 18-27 ° C for 22-26 hours by shaking all the time. After that, the cells were collected by centrifuge (Centrifuge) at a speed of 3,000-5,000 rpm for 10-20 minutes, washed the cells with clean water 1-2 cycles and dried at 70-80 degrees Celsius for a time. 30-40 minutes and stored a single cell protein (Single Cell Protein) in a container ------------ 1. Formula and process for producing a single cell protein (Single Cell Protein) for use as Raw material of food protein The animal has the following processes: a. Substrate preparation which consists of water, starch left over from production. And the rest of the water from Steaming process The remaining starch water from the production was used to digest the starch into glucose (Glucose) with an enzyme amylase (Enzyme Amylase) ratio 1-2% for 3-4 hours, then bring both types of water into centrifugation ( Centrifuge) at a speed of 3,000-5,000 rpm for 10-20 minutes, the clear parts of both types of water were sterilized at 121 ° C for 15 minutes while the sludge of both types of water was dried at 70. -80 degrees Celsius for 2-3 hours. B. The precursor preparation process of yeast strain Candida utilis (Candida utilis) was isolated from Production process in the poultry processing industry The yeast strains Candida utilis were cultured on the Potato Dextrose Agar (PDA) medium and then incubated at The temperature was 34-36 ° C for 22-26 h, after which Candida utilis was removed from Potato Dextrose Agar (PDA). 1 loop is placed in 100 ml of Yeast Extract Peptone Dextrose Broth (YPD) culture medium and then incubated at 18-27 ° C for time. 22-26 hours with shaking all the time After that, the Candida utilis solution was applied to adjust the concentration. With Yeast Extract Peptone Dextrose Broth (YPD) culture medium at 0.50-0.55 Mechfaland (McFarland) C. Process for single-cell protein production ( Single Cell Protein) by mixing both types of sterilized water. The ratio of water, starch residue from production of 20-40% and residual water from the process of 60-80%, then the starter of the yeast strains Candida Utilis (Candida). utilis) at a concentration of 0.50-0.55 McFarland (10-20% McFarland) was added to 80-90% Substrate, incubated at 18-27 ° C for 22-26 hrs by shaking all the time. After that, the cells were collected by centrifuge (Centrifuge) at a speed of 3,000-5,000 rpm for 10-20 minutes, washed the cells with clean water 1-2 cycles and dried at a temperature of 70-80 degrees Celsius for a time. 30-40 minutes and stored a single cell protein (single cell protein) in a container.