TH164201A - Systems and methods for the detection of cells using transfer particles. Through genetic engineering - Google Patents

Abstract

Herein describes systems and methods for detecting and / or identifying target cells (eg bacteria) using genetically engineered transfer particles in certain sample characteristics. To transfer a certain amount into a sample Transfusion particles Transfer is relative to the target cell The transfer particles are not self-replicating and are genetically engineered to incorporate formulated nucleic acid molecules to make target cells produce them. Set of reporter molecules Keep sample and transfer particles in order to express a set of molecules. Reporter when target cells are in the sample Accepts signals that are associated with a number of reporter molecules. In some examples The size of the signal is independent of the number transfer particles. One of the above in the preset number

Claims (1)

: Here describes systems and methods for detecting and / or identifying target cells (e.g. bacteria) using genetically engineered transfer particles in certain sample characteristics. Methods include particle mixing. To transfer a certain amount into the sample Transfusion particles Transfer is relative to the target cell The transfer particles are not self-replicating and are genetically engineered to incorporate formulated nucleic acid molecules to make target cells produce them. Set of reporter molecules Keep sample and transfer particles in order to express a set of molecules. Reporter when target cells are in the sample Accepts signals that are associated with a number of reporter molecules. In some examples The size of the signal is independent of the number transfer particles. One of the above in the pre-defined amount. (Item one) which will appear on the advertisement page: 1. Methods included: Mixing a set of transfer particles in relation to the target cell with the sample. The set of transfer particles is through genetic engineering to combine formulated nucleic acid molecules. To make Target cells produce a series of reporter molecules. In which a set of transfer particles adjust the formula to It binds to and delivers nucleic acid molecules to target cells. Where the set of particles in a tag transfer: