TH14070C3 - Bioethanol production process using Monascus from the macroalgae group - Google Patents

Abstract

------ 16/02/2018 ------ (OCR) page 1 of the number 1 page invention summary. Bioethanol production process using monascus fungi from this macroalgae group by using inoculum. Genus Monoscus (Monoscus sp.) By cultivating the fungi in the culture medium and extracting the dried algae from the macroalgae group until the water was extracted from the macroalgae group. Algae extract Conduct an ethanol test With enzyme technique Specific reaction type The invention is intended to produce bioethanol. For renewable energy from oil fields Petroleum, which will be reduced until the end of the future. Using raw materials from seaweed as an alternative of raw materials Due to the high content of starch and cellulose Does not affect the food crops like other raw materials such as cassava, corn and when used to produce biodiesel, it can also be used to produce bioethanol. ------------ New revised September 15, 2017 Bioethanol production process using Monascus fungi from this macroalgia group using the genus Monascus strain (Monascus sp.) The fungi were cultured in the culture medium and the macroalgae dried algae were extracted until the water was extracted from the macroalgae group. Water to extract algae Conduct an ethanol test With enzyme technique Specific reaction type The invention is intended to produce bioethanol. For renewable energy from oil fields Petroleum, which will be reduced until it is exhausted in the future. Using raw materials from seaweed as an alternative of raw materials Due to the high content of starch and cellulose It does not affect the food crops like other raw materials such as cassava, corn, and when it is used to produce biodiesel, it can still be used to produce bioethanol. -------------------------------------------------- ---------------------- (Old) This process was used to produce ethanol from raw materials from macroalgae resources using fibrous fungi. Genus Monascus (Monascus sp.) Is intended to produce bioethanol as a renewable energy for oil from the petroleum source, which will be depleted in the future. Using raw materials from Seaweed is an alternative to raw materials due to its high content of starch and cellulose. Does not affect the plant Food is the same as other ingredients. Such as cassava, corn, and when used to produce biodiesel, it can still Can be used to produce bioethanol.

Claims (1)

Disclaimer (all) which will not appear on the advertisement page. : ------ 16/02/2018 ------ (OCR) Page 1 of 1 page Disclaimer 1. Nioethanol production process using Monascus from algae. Macro Alge Group It consists of the following steps: preparation of monascus seedlings. Preparation of monascus fungi. In medium containing 40-200 grams of rice grits and 24-1200 ml of water were cultured at 25-55 degrees Celsius for 5 days in a culture container. And cover the container with sterile method. B. Apply 1-50 g of monascus from Article A. (at least 107 CFU / ml (CFU / ml) in a phosphate buffer. ) Concentration of 0.1 molar, pH equal to 7, volume 99 ml, min. 0.1% polysorbate (Polysorbate 80: Tween 80), mixed in an autoclave container and then spore distribution. By using a circular shaker, the spores were dispersed from the rice kernels for 10-15 minutes. Preparation of algae extraction. C. 3 -100 g of dried algae from the macroalgia group, add hydrochloric acid (HCI). ) Concentration 1.25 molar volume 15 ml and distilled water 50 ml. Sterilized at 121 ° C at 15 pounds of pressure per square for 15 minutes. D. Alkalinity equal to 7 with 15 percent sodium hydroxide (NaOH). Add 1.8 g of peptone and 0.9 g of yeast extract. Adjust the volume to 90 ml with distilled water. The water was then sterilized at 121 degrees Celsius at 15 pounds per square inch for 15 minutes. The water was extracted from the macroalgae dry algae group. Fungi culture for ethanol production. E. The algal extraction water obtained from Article 100 - 1000 ml. Per spores of Monascus seedlings from item B. Volume 1-100 ml. Temperature 30 -35 degrees Celsius on the shaker to make Circular air with a speed of at least 100 - 300 rpm for a period of 3-7 days. F. Ethanol testing. With enzyme technique Model with specific reaction characteristics Specifically, ethanol formed from the production process and co-injection technique. (Co-injection) by using a chromatography tool (High performance liquid chromatography (HPLC) to test, confirm type and measure ethanol content ------------ Revised 15 Sep. 2017 1. Bioethanol production process using Monascus fungi from macroalgae group. It consists of the following steps: preparation of monascus seedlings. Preparation of monascus fungi. In medium containing 40-200 grams of rice grits and 24-1200 ml of water were cultured at 25-55 degrees Celsius for 5 days in a culture container. And cover the container with sterile method. B. Apply 1-50 g of monascus from Article A. (at least 107 CFU / ml (CFU / ml) in a phosphate buffer. ) Concentration of 0.1 molar, pH equal to 7, volume 99 ml containing 0.1 percent polysorbate (Polysorbate 80: Tween 80), mixed in a sterilized container and then spore distribution. By using a circular shaker, the spores were dispersed from rice kernels for 10-15 minutes. Preparation of algae extraction. C. 3 -100 g of macroalgae dry algae, add hydrochloric acid (HCl ) Concentration 1.25 molar volume 15 ml and distilled water 50 ml. Sterilized at 121 degrees Celsius at 15 pounds per square inch for 15 minutes. D. Take the solution from the note C. Adjust the pH. To equal 7 with 15 percent sodium hydroxide (NaOH), add 1.8 grams of peptone and 0.9 grams of yeast extract. Adjust the volume to 90 ml with distilled water. The water was then sterilized at 121 degrees Celsius at 15 pounds per square inch for 15 minutes. The water was extracted from the macroalgae dry algae group. Fungi culture for ethanol production. E. The algal extraction water obtained from Article 100 - 1000 ml. Per spores of Monascus seedlings from item B. Volume 1-100 ml. Temperature 30 -35 degrees Celsius on the shaker to make Circular air with a speed of at least 100 - 300 rpm for a period of 3-7 days. F. With enzyme technique Model with specific reaction characteristics Specific to the ethanol produced from the production process and co-injection technique. (Co-injection) by using a chromatography tool (High performance liquid chromatography (HPLC) to test, confirm the type and measure the ethanol content. -------------------------------------------------- --------------------- (original) 1. Bioethanol production process using Monascus fungi from the macroalgae group. It consists of the following steps: Preparation of monascus seedlings. Preparation of cultivating Monascus. In medium containing 40- ............ gram, 24-1200 ml of water, cultured at 25-55 ° C for 5 days in a culture vessel. And cover the container with sterile method. B. Apply 1-50 g of monascus from Article A. (at least 107 CFU / ml (CFU / ml) in a phosphate buffer. ) Concentration of 0.1 molar, pH equal to 7, volume 99 ml containing 0.1 percent polysorbate (Polysorbate 80: Tween 80), mixed in a sterilized container and then spore distribution. By using a circular shaker, the spores were dispersed from rice kernels for 10-15 minutes. Preparation of algae extraction. C. 3 -100 g of macroalgae dry algae, add hydrochloric acid (HCl ) I concentrated 1.25 molar volume 15 ml and distilled water 50 ml. Sterilized at 121 ° C at 15 psi pressure for 15 minutes. D. Remove solution from d. Adjust the acidity - Alkalinity equal to 7 with 15 percent sodium hydroxide (NaOH). Add 1.8 g of peptone and 0.9 g of yeast extract. Adjust the volume to 90 ml with distilled water. The water was then sterilized at 121 degrees Celsius at 15 pounds per square inch for 15 minutes. The water was extracted from the macroalgae dry algae group. Fungi culture for ethanol production. E. The algal extraction water obtained from Article E, volume 100 - 1000 ml per spores of monascus seedlings from 1-100 ml. Temperature 30 -35 degrees Celsius on the shaker to make Circular air with a speed of at least 100 - 300 rpm for a period of 3-7 days. F. Ethanol testing. With enzyme technique Model with specific reaction characteristics Specifically, ethanol formed from the production process and co-injection technique. (Co-injection) by using a chromatographic tool (High performance liquid chromatography (HPLC)) to test the genes and measure ethanol content. Will have at least 1 gram of ethanol per kilogram of weight Dried seaweed