TH105498B - The process of producing seed germination stimulants and plant growth promoters from Jatropha curcas seed cake using biotechnology. - Google Patents

Abstract

------October 25, 2024------(OCR) OCR 09WP This invention relates to the process of producing seed germination stimulants and plant growth promoters from jatropha seed cake, a waste product from the oil extraction process. The process begins with separating toxins from the jatropha seed cake, separating proteins from the separated proteins, and hydrolyzing the separated proteins to obtain extractable proteins that can be used as seed germination stimulants and plant growth promoters. These are high-value substances with applications in agriculture.

Claims (1)

------October 25, 2024------(OCR) OCR 09WP1. The process of producing seed germination stimulants and plant growth promoters from Jatropha curcas seed cake using biotechnology consists of 3 steps as follows: Step 1: Removal of toxins from Jatropha curcas seed cake using ethanol. Weigh the Jatropha curcas seed cake into a container, add 90% ethanol in a ratio of 1:3 (w/v), shake at a speed of 150 rpm for 5 minutes. Then filter the suspended solids with filter paper, discard the clear liquid, and then extract the remaining Jatropha curcas seed cake through 90% ethanol three more times, each time for 5 minutes. This will obtain a toxin-free Jatropha curcas seed cake. Step 2: Separation of proteins from the toxin-free Jatropha curcas seed cake by adjusting the pH. The toxin-free Jatropha curcas seed cake is finely ground. The dried protein was dissolved in water at a ratio of 1:10 (w/v), left at room temperature for 30 minutes, and the pH adjusted to 12 using 1 M concentrated sodium hydroxide. It was stirred continuously for 3 hours at 50°C, then centrifuged to separate the precipitate at a force of 12,000 xg for 10 minutes. The resulting clear liquid was collected, its pH adjusted to 4 using 1 M concentrated hydrochloric acid, and centrifuged to separate the precipitate at a force of 12,000 xg for 10 minutes. The precipitate was then freeze-dried (lyophilization). Step 3: Production of extracted protein from the protein isolated from the Jatropha curcas seed cake using protease enzyme. A 1% (w/v) protein solution was prepared in a 0.1 M phosphate buffer solution at a pH of 7. The protein was then hydrolyzed using a 1% enzyme solution at a protein-to-enzyme ratio of 100:1 (v/v) at an optimum temperature of 50°C. The reaction was incubated for 2 hours, then stopped by heating at 90°C for 10 minutes. The precipitate was then separated by centrifugation at 4°C with a centrifugal force of 12,000 xg for 15 minutes. The clear protein extract was collected for use as a seed germination stimulant and plant growth promoter.