TH103552A - Method for the production of L-thyronine - Google Patents

Abstract

DC60 (06/09/49) A method for the production of L-theronine has been described, including the micro-culture process. Life belonging to the Escherichia family capable of producing L-thyronine. In the medium used to Fermentation containing carbon source, nitrogen source And sulfur sources And the collection of L-Theonine Where the sulfur concentration in the medium is controlled so that it is at a predetermined level or lower, the methods for L-theronine production have been described including the process of culture. micro- Life belonging to the Escherichia family capable of producing L-thyronine. In the medium used to Fermentation containing carbon source, nitrogen source And sulfur sources And the collection of L-Theonine Where the sulfur concentration in the medium is controlled so that it is either at a predetermined level or lower.

Claims (9)

Disclaimer (all) which will not appear on the advertisement page: EDIT 12/06/15 1. Methods for producing L-Theonine. Contains: A) Escherichia microorganism cultures capable of producing L-theronine, in which the fermentation medium is composed of a carbon source, a tritogen source and a sulfur source. B) Collection of L-Theraonine from culture media Where the sulfur concentration in the medium is Control to be 0.35 g / l or less 2. Method for claim 1 or 2 where the microorganism is Escherichia coli 3. Method according to one of the claims 1 to 2 where Enzyme used for synthesis The biological activity of L-Theraonynonovirus was modified so that the enzyme was unacceptable to control. Reversed by L-Theronine 4. Method according to claim 4 whereby the enzyme used in biosynthesis L-Theronine These enzymes were selected from a group containing aspatokinase, homoserene kinase, theronin synthase, and their combination of enzymes. 5. What method of claim? One of points 1 to 5 where the sulfur source was chosen from Group containing sulphate, thiosulfate, sulfite, cysteine, cystine, glutathione and a combination thereof. 6. Method according to one of the claims of At 1 to 6 where the culture method is chosen from The group consists of the batch culture method, the batch culture method with the addition of feed during culture, and the continuous culture method 7. Method according to claim 7, whereby the Culture is a batch culture method that has Add more food while culturing Or continuous culture method Where the food medium It consists of a source of sulfur which is added to the culture media in the fermenter. 8. Method of claim 8, whereby such food intermediates also contain carbon sources and nutrients that promote growth. And with the food medium being Continuously added to the culture media in the fermenter Or periodically So that the concentration of the source Carbon in the culture media is maintained at 30 g / l or less. After the end of 9. A method for the production of feed additives based on the culture water used in the production of feed additives. Fermentation containing: A) Culture of Escherichia microorganisms capable of producing L-theronine in carbon-containing fermentation medium, trogen source. B) fermentation operations where the sulfur concentration in the medium is controlled so that The concentrate is either at or below the predetermined level. C) Treatment of unprocessed fermentation media to obtain water content of 10% or less by weight 1 0. According to claim 10 or 11, where the microorganism is Escherichai coli 1 1. Method of claim for Clause 9 to 10, where the enzyme used for Biosynthesis The microbial L-theronine is modified so that the enzyme does not enter its inhibition. Reverse by L-Thionine 1 2. Method according to claim 13 whereby the enzyme used in biosynthesis Onine was selected from a group consisting of aspatokinase, homoserine kinase, theronin synthase and a combination of those enzymes. Any one of the rights of Sections 10 through 14 where the subwoofer source is selected From a group containing sulfate, thiosulfate, sulfite, cysteine, cystine, glutathione, and any combination thereof.1 4. Method of one of the claims. Of items 9 to 13 where the culture method was selected From the group consisting of batch culture method, batch culture method with feeding Increase while raising And continuous culture method 1 5. Method of aquaculture claim 16 where the culture method is batch culture method with Adding more food while culture Or continuous culture method Where the food medium It consists of a sulfur source added to the culture media in the fermenter 1 6. Method of claim 16 where such food medium also contains carbon sources and nutrients that promote growth. And with the food medium being Continuously added to the culture media in the fermenter Or periodically So that the concentration of the source Carbon in the culture media is maintained at 30 g / l or less. After the end of Thrive during microbial lock 1 7. Method for one of the claims 9 through 16 where more than 95% of L-Thearonine is included in the feed additive. -------------------------------------------------- ----------- 1. Methods for the production of L-theronine Contains: A) Escherichia microorganism cultures capable of producing L-THERONINE, in which the fermentation medium is composed of a carbon source, tritogen source. And sulfur source. B) Collection of L-theronine from culture media. Where the sulfur concentration in the medium is controlled This is so that it is at a predetermined level or lower than 2. Method of claim 1 where the sulfur concentration in the fermentation medium is The control was at 0.35 g / l or lower. 3. Method of claim 1 or 2, where the microorganism is Escherichia coli. 4. Method according to one of the claims 1 to 3, whereby Enzyme used for synthesis The biological activity of L-Theraonynonovirus was modified so that the enzyme did not accept back control. Back by L-Theraonine 5. Method according to claim 4 whereby the enzyme used in biosynthesis These enzymes were selected from a group containing aspatocinase, homoserine kinase, theronin synthase, and their combination of enzymes. 6. What method of claim? One of points 1 to 5 where the sulfur source was chosen from Group containing sulphate, thiosulfate, sulfite, cysteine, cystine, glutathione, and a combination thereof 7. Methods according to one of the claims At 1 to 6 where the culture method is chosen from The group consists of batch culture method, batch culture method with additional feeding during culture and continuous culture method 8. Method of claim 7, whereby the Culture is a batch culture method that has Add more food while culturing Or continuous culture method Where the food medium Contains a source of sulfur which is added to the culture media in the fermenter 9. Method of claim 8, whereby such food medium also contains carbon and nutrient sources that promote growth. And with the food medium being Continuously added to the culture media in the fermenter Or periodically So that the concentration of the source Carbon in the culture media is maintained at 30 g / l or less. After the end of growth It is a method for the production of feed additives based on the culture fluids used in the microorganisms. Fermentation containing: A) Culture of Escherichia microorganisms capable of producing L-threonine in fermentation medium containing carbon source, trogen source. B) fermentation operations where the sulfur concentration in the medium is controlled so that The concentrate will be at a predetermined level or lower than C) Drying culture media used in unprocessed fermentation to contain 10% or less water content by weight 1. 1. Method according to claim 10, wherein the sulfur concentration in the fermentation medium. Is controlled so that the concentration is 0.35 g / l or less than 1 2. Method according to claim 10 or 11 where the microorganism is Escherichai coli 1. 3. Method according to any claim of Articles 10 through 12, whereby the enzyme Biosynthesis: L-Theronine in microorganisms is modified so that the enzyme does not enter the reverse inhibition. Back by L-Thionine 1 4. Method according to claim 13, whereby the enzyme used in biosynthesis L-THERO Nene was selected from a group consisting of aspatokinase, homoserine kinase, theronin synthase, and a combination of those enzymes. 5. Method for one of the claims 10 to 14 where the subwoofer source is selected. From a group containing sulfate, thiosulfate, sulfite, cysteine, cystine, glutathione and their combination 1 6. Methods based on any of the claims 10 through 15 where the culture method is selected. From the group consisting of batch culture method, batch culture method with feeding Increase while raising And continuous culture method 1 7. Method according to claim 16, where the culture method is the batch culture method with Adding more food while culture Or continuous culture method Where the food medium Contains a sulfur source which is added to the culture media in fermentation tanks 1. 8. Method for claim 16, whereby such food intermediates still contain carbon and nutrient sources that promote growth. And with the food medium being Continuously added to the culture media in the fermenter Or periodically So that the concentration of the source Carbon in the culture media is maintained at 30 g / l or less. After the end of growth Grow during the lock of microorganisms 1 9. Any claim method of Sections 10 through 18 where more than 95% of L-Thearonine is included in the said food additive.