SU983141A1 - Method of producing cellulolytic enzyme complex - Google Patents
Method of producing cellulolytic enzyme complex Download PDFInfo
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- SU983141A1 SU983141A1 SU813307248A SU3307248A SU983141A1 SU 983141 A1 SU983141 A1 SU 983141A1 SU 813307248 A SU813307248 A SU 813307248A SU 3307248 A SU3307248 A SU 3307248A SU 983141 A1 SU983141 A1 SU 983141A1
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- SU
- USSR - Soviet Union
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- cultivation
- producer
- culture
- cellulase
- fungus
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- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
С54) СПОСОБ ПОЛУЧЕНИЯ КОМПЛЕКСА ЦЕЯПЮЛОЛИТИЧБСКИХ Изобретение относитс к ферментно отрасли микробиологической промышленности и может быть исполь.зовано дл гид ролиза целлюлозосодержащего сырь в кормопроизводстве при силосовании гру бых кормов,в пищевой промы }шенности,а также дл получени сахаристых растворов из отходов пищевой и деревообрабатывающей промышленности. Известен способ получени микробного белка культивировани сме шанной культуры Cellulomonas sp. Alcaligenes facalis. Приводитс одновременный посев-обеих культур на питательную среду, содержащую продукты -щелочного гидролиза измельченных городских и сельскохоз йственных отходов, и их дальнейшее выращивание при 30° С 1 . Недостатком этого способа вл .етс то, что культивирование не сопровождаетс накоплением фермента Известен способ получени целлюлозы и микробного белка, предусматривающий совместное культивирование глубинным сттособом Trichoderma vlrl .de 44 и дрожжей при одновременном .внесении посевных культур микроор- . ганизмов в ферментационную среду. Культивирование осу -;ествл етс приC54) TSEYAPYULOLITICHBSKIH METHOD FOR COMPLEX This invention relates to the microbiological industry enzyme industry and can be ispol.zovano guide for Roliz cellulose feedstock in fodder with fodder ensilage GRU-singular, the food ind} shennosti as well as to obtain solutions of sugar waste food and wood industry . A known method for producing a microbial protein is the cultivation of a mixed culture of Cellulomonas sp. Alcaligenes facalis. Simultaneous sowing of both cultures on a nutrient medium containing products of alkaline hydrolysis of crushed urban and agricultural wastes is given, and their further cultivation at 30 ° C 1. The disadvantage of this method is that the cultivation is not accompanied by the accumulation of the enzyme. There is a method of producing cellulose and microbial protein, which involves the co-cultivation of the Trichoderma vlrl .de 44 deeper method and yeast while simultaneously sowing the microoric cultures. ganizmov in a fermentation medium. Cultivation of the wasp is;
ФЕРМЕНТОВ 2 . 30°С в течение 7-13 сут (максимальное накопление белка и целлюлазы на дес тые сутки ) С2. . Однако полученный комплекс ферментов практически не содержит цел-, лобиазу и пёктинаэу, необходимые дл наиболее полного гидролиза целлюлозо-. содержащего сырь .. Недостатком способа вл етс также длительность культивировани . Цель изобретени - полный и экономичный гидролиз целлюЛоэосодержащего сырь и сокращение сроков культивировани продуцентов. Поставленна цель достигаетс тем, что согласно способу получени комплекса целлюлолитических ферментов, предусматривающему совместное культивирование двух культур глубинным способом, совместному культивированию подвергают культуру гриба Aspergillus foetldus М-45 - пролуцента целлобиазы и аектиназы и культуру гриба Trichoderma viride - продуцента целлюлазы, причем в последнюю внос т первый продуцент в конце зкспоненцирльной фазы роста и продолжают совместное культивирование; в течение З-б суток.ENZYME 2. 30 ° С for 7–13 days (maximum accumulation of protein and cellulase on the tenth day) C2. . However, the resulting complex of enzymes contains almost no cel-, lobyase and pictoenac needed for the most complete hydrolysis of cellulose. containing raw materials. The disadvantage of this method is also the duration of cultivation. The purpose of the invention is a complete and economical hydrolysis of cellulose-containing raw materials and a reduction in the time of cultivation of producers. The goal is achieved by the fact that according to the method of producing a complex of cellulolytic enzymes, involving co-cultivation of two cultures by a deep method, co-cultivation is subjected to a culture of the fungus Aspergillus foetldus M-45 - a cellobiase and aectinase fungus and a culture of the fungus Trichoderma viride, a producer of cellulase, and a human. the first producer at the end of the exponential growth phase and continue co-cultivation; during the B-day.
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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SU813307248A SU983141A1 (en) | 1981-06-29 | 1981-06-29 | Method of producing cellulolytic enzyme complex |
Applications Claiming Priority (1)
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SU813307248A SU983141A1 (en) | 1981-06-29 | 1981-06-29 | Method of producing cellulolytic enzyme complex |
Publications (1)
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SU983141A1 true SU983141A1 (en) | 1982-12-23 |
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Family Applications (1)
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SU813307248A SU983141A1 (en) | 1981-06-29 | 1981-06-29 | Method of producing cellulolytic enzyme complex |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994026880A1 (en) * | 1993-05-10 | 1994-11-24 | Gist-Brocades N.V. | Combined action of endoglucanases and cellobiohydrolases |
-
1981
- 1981-06-29 SU SU813307248A patent/SU983141A1/en active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994026880A1 (en) * | 1993-05-10 | 1994-11-24 | Gist-Brocades N.V. | Combined action of endoglucanases and cellobiohydrolases |
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