SU939407A1 - Process for biologically purifying effluents from phenolic compounds - Google Patents

Description

The invention relates to wastewater treatment methods. Water from toxic phenolic compounds and products of their oxidative transformations - chinomes and can be used in the wastewater treatment of pulp and paper, oil and coal chemical industries.

A known method of purification of wastewater from Lenol extraction. Butyl acetate, benzene, etc. are used as the extractant. L.

A known method of wastewater treatment by introducing a plant tissue homogenate. Tissue algae or aquatic plants or plant waste materials are used as crop tissues. The method is used in pulp and paper, petrochemical and chemical and other industries of the industrial network 2.

The way it is done by adding 1 g of a freshly prepared growing homogenate of tissue to 100 ml of a solution of the phenol or p-benzoquinone under study at a concentration of 10, 10, 10 J4 and below. The mixture is shaken, sampled and analyzed. 1.2.

The disadvantages of this method are the rapid inactivation of homogenates during storage, the presence of secondary contamination with plant ballast materials, and the need for 0.5-1 hour contact to achieve 90-95% purity.

The purpose of this is to accelerate the process of cleaning and the elimination of secondary contamination with ballast and substances.

Delivered. The goal is achieved by the fact that in the method of biological treatment of wastewater from phenolic compounds into wastewater, it enters enzyme preparations isolated from plant tissue homogenates, which are pretreated with acetone or phosphate buffer.

20

Enzyme preparations are prepared by washing the plant tissue homogenate with acetone until the wash water remains colorless. The resulting powder is dried and used. CDOK hr chvni nooot-jka with unchanged activity of 8 months. to 1 g. Protein preparation pol5, ayut, rubbing vegetable tissue in phosphate buffer. Then squeeze the brain

through gauze n solution is used in cleaning.

The isolated enzyme prepaoat consists of three components with molecular masses of 38-40000, 75-77000 and 140-180000. The specific activity for chlorogenic acid is 586.19 mg mol / minmg of protein. The copper content of 0.23-0.25%.

The acetone preparation is obtained by washing 10 g of the plant homogenate until complete bleaching and is dried.

Acetone recovery is 80% of the original. The output of acetone 15 powder 5-15% of the original raw gomegenat.

When extracting 10 g of plant homogenate, 50 ml of phosphate buffer (pH 6.75) is poured and squeezed through gauze. The shelf life of the received plant is 10-12 days. Dp is more prolonged (the storage solution is treated with the ammonium ammonium atom, the protein and cviuaT are collected. The shelf life of the dried preparation at a temperature of 5-10 seconds to 3 months.

P. Reamer. 0.1 g of an acetone preparation or 10 ml of extract is poured over 100 ml of the solutions of the phenols under study (), shaken and samples are taken for analysis of phenols using gas-liquid chromatography or a floor system.

The results for the degree of purification from phenols and quinones are given in table.1.

Table 1

Acetone preparation from algae Nitella

Acetone preparation from the top layer of potato tubers

Acetone preparation from the fabric of seedlings of sunflower

8.2

17.0 31.4

72.3 58.6 72.2

75.6

83.7 78.0 85.0

16.1 35.3 57.4

82.9 76.2 85.6

86.7

90.0 96.6 92.2

29.6 63.0

74.2

80.0 82.5

89.1

90.6

92.7 93.8 95.3

Comparative time of contact of a solution of phenols with homogenates, acetone preparations and extracts of nitel. Continuation of table. one

table, for 85% of the degree of purification are given in Table. 2

table 2

Contact time for 85% cleaning, min

Claims (1)

Claim The method of biological treatment of wastewater from Phenolic compounds by homogenates of plant tissues, characterized in that, in order to accelerate the process of purification and eliminate secondary pollution with ballast substances, Enzyme preparations isolated from plant tissue homogenates that are pretreated with acetone are introduced into the wastewater or phosphate buffer.