SU938873A1 - Composition for preservation of donor kidneys - Google Patents

Description

(5) COMPOSITION FOR CONSERVATION OF DONOR KIDNEYS

The invention relates to a median, namely, clinical and experimental surgery, and can be used for the preservation of isolated donor kidneys intended for subsequent transplantation and extracorporal connection. A known composition for the conservation of donor kidneys, containing potassium salts, sodium succinate, sodium chloride, glucose and heparin 1. However, the known composition does not ensure the maintenance of metabolic activity in an isolated kidney and the maintenance of its viability. The purpose of the invention is to maintain the metabolic activity in an isolated kidney and preserve its viability. This goal is achieved by the fact that the composition for the conservation of donor kidneys containing potassium salts, sodium succinate, sodium chlorides, glucose and heparin, additionally contains adenine. sodium arbonate, sodium acetate, o- and disubstituted potassium phosphate in the following ratios of components, g / l: Odosubstituted phos1, 025-1.030 fats of potassium bi-substituted 3.7-3.8 potassium phosphate 0.8it-0.86 sodium bicarbonate 0, 5-0.55 Sodium succinate 0.6-0.65 Sodium acetate 0.5-0.55 Sodium chloride Adenine (ATP) 0.01-0.012 0.25-0.3 G; parin i (8-50 Glucose Distilirododo water 1000 ml bath. Characterization of the composition for preservation of donor kidneys to the minimum, optimal and maximum control of its ingredients by bringing the table.

3938873

 -V-V - Solution composition Minimum I Optimum

is unacceptable

OOA 0.05

below 0.001 not effective 0.0012

microthrombosis occurred below 0.02 - P, 025-0.003. It is necessary to maintain the pH of the solution within 7, and the concentration of K-50-55 meq / l ions and osmolarity within 310-330 masm / l. A decrease in sodium succinate below 0.0 leads to a substrate and thereby impairs preservation results, an increase in the concentration of 0.06 above does not change the results; however, at 0.1, biochemically evaluated tissue respiration decreased, the creatinine level in the blood was higher. A decrease in sodium acetate below 0.5 results in substrate hunger (reduction in tissue respiration), above 0.09 it also reduces tissue respiration. When the concentration of adenine is 0.002, peroxidation of the lapid membrane structures is enhanced, resulting in damage to the epithelium of the canalicular apparatus. Excess of heparin concentration is unacceptable because the concentration of carbolic acid, which is part of heparin, increases and manifests its toxic properties. The glucose concentration should not change due to the inadmissibility

four

1 Maximum

0,102-0,103

0.37-0.37

is unacceptable

0,084-0,086

0.05-0.055

0.06 0.06-0.065 0.09

0.002 0.06

Claims (1)

, 8-5.0 to 100 ml, with the change in the optimal osmolarity of 310-330 mas / l. Prepare And apply the composition as follows. 875 ml of distilled water (in the absence of adenine in-preservative solution is added before consuming; re-adding 1 ml of 1% ATP solution) is added to a portion of mono- and disubstituted potassium phosphate, sodium bicarbonate, succinate, acetate, and sodium chloride, adenine. After complete dissolution of the listed components, the composition is filtered and autoclaved. The sterilized formulation as a prefabricated is stored in the refrigerator. Before removing the donor kidney to a cooled semi-finished product, 125 ml of glucose solution and 0.25 ml of heparin are added from sterile ampoules. The prepared composition (1 l) is washed off the extracted donor kidney, then immersed in a transport container in which the temperature is maintained at 0-2s for part 5. The use of the proposed composition for the conservation of donor kidneys increases the permissible conservation time to 36-48 h, which allows immunological selection of transplant recipients; reduces the number and reduces the incidence of pesticheskimi disorders of the graft due to the resuscitation of the graft and the prolongation of metabolism in it; and significantly reduces the duration of stay of patients in the clinic after kidney transplantation. DETAILED DESCRIPTION OF THE INVENTION Composition for the conservation of donor kidneys, containing potassium salts, sodium sugary sodium, sodium chloride, glucose and heparin, characterized in that, in order to maintain metabolic activity in an isolated kidney and maintain its viability, it additionally contains adenine, sodium bicarbonate, acetate sodium, monobasic and disubstituted potassium phosphate in the following ratios of components, g / l: Monosubstituted phos1, 025-1.030 fats of potassium; Disubstituted phos3; 7-3.8 fats of potassium 0.84-0.86 sodium bicarbonate / 0.5- 0.55 sodium succinate 0.6-0.65 sodium acetate 0.5-0.55 Sodium chloride Adenine (ATP) 0.01-0.012 0.25-0.3 Heparin 48-50 Glucose Distilled water 1000 ml. on Sources of information taken into account during the examination 1. Shumakov V.I. and others. Preservation of organs. M., 1975, p. lOif-237.