SU905694A1 - Device for preparation of objects for ultra microtome - Google Patents

Description

(5) DEVICE FOR PREPARING OBJECTS FOR ULTRAMICROTES

The invention relates to devices and accessories for ultramicrotomes and can be used in the instrument-making industry and the medical equipment industry. When producing sections of elastic materials, for example, biological, they need to be given a certain stiffness and hardness, for which the object (small pieces of material about 1x1 mm in size) is impregnated with polymer in medical gelatin ampoules of various sizes. The block obtained after polymerization has the shape of a cylinder with a spherical bottom, where the object is located. After polymerization, the block is prepared for cuts, for which a truncated pyramid with a top side size of 0.2x0.2 mm and a height of 1-1.5 mm is made at the location of the object by using a safety razor or other cutting tool under a light microscope. This process is labor intensive, requires highly skilled operator and results in significant sample loss. A special device TM-60, known for cutting pyramids, is known; it mechanizes the labor-intensive process of making pyramids 1. However, this device is structurally complex and labor-intensive to manufacture, it uses moving mechanisms and cutting tools, which is unsafe. In addition, it has a low pyramid production capacity and in the pyramid manufacturing process, a large amount of harmful dust of epoxy resin or other polymers is released. Closest to the invention is a device containing deep matrices (similar to medical capsules) located on a pallet. Such a device, due to the presence in the pan of several dies, allows you to simultaneously cook several

fills and provides easy removal of polymerized blocks from the forms by crushing the cells with fingers 2.

The drawbacks of the device are the large depth cells of the matrix, which does not allow simultaneously observing and orienting the object under the microscope when laying in the cell and filling with polymer; object.

The purpose of the invention is to improve the performance, productivity and quality of the preparation of objects.

This goal is achieved by the fact that in a device for preparing objects for ultramicrotomes containing a matrix, the matrix is made of two parts made in the form of discs, the bottom of which is made with recesses with a transparent bottom, and the top is made with. holes coaxial grooves

To facilitate the removal of the object polymerization material, the disks are made of a material with low adhesion to the polymer (poorly wetted by the polymer), while the upper disk is made of an elastic material, for example, rubber.

FIG. 1 shows the proposed device, a cross-section; in fig. 2 - the process of orientation and installation of the object into the recess of the lower disk.

The object preparation device is made in the form of a matrix consisting of the lower part 1, having a recess 2 in the form of a truncated pyramid with a transparent bottom 3 through which the object is illuminated. On the upper part 4 there are holes 5 coaxially with depressions in the lower part. Both parts of the die are fastened together by a detachable joint.

The device for preparing objects for ultramicrotomes operates as follows.

For the orientation and placement of the object, the lower part 1 is installed on the table of the light microscope 6 of the MBS type and illuminated from below so that in the transmitted light of the xopoiuo the contour of the bottom of the recess is visible as well. the luminous flux passing through the bottom is 056944

Accurate to illuminate object 7. Observe the 8 microscope 8 and use a pointed tweezers 9 or a needle to orient and stack the object on the bottom of the 5 grooves. After laying the object is fixed with a drop of polymer. After all the pyramidal depressions of the lower disk are filled with samples, and the latter are fixed, with the mouth 0, the upper disk fills and fastens with the lower one so that the central axes of the pyramidal depressions and holes coincide. Combined cavities in both disks are filled.

5 polymer undergoing polymerization under certain temperature conditions. To remove the polymer blocks, the upper and lower discs are disconnected from the objects. Due to the low adhesion and shallow depth of the recesses of the lower disc, polymer blocks are easily removed from the recesses. Upper disk made of elastic, poorly wetted by polymer

J5 of the material allows the resulting polymer blocks to be removed by bending the disc. One end of the obtained polymer blocks has the shape of a truncated pyramid, at the apex of which

The object is located oriented.

Thus, in the proposed device, the matrix is divided into two parts, one of which (lower) forms a pyramid with the object, and the second (upper) - tail section of the polymer block. The shallow depth and the transparent bottom of the depressions (lower part) in the form of a truncated pyramid create convenience for orientation, placement, fixation of the object and extraction of polymer blocks. The alignment of the holes in the upper part of the matrix and its execution from an elastic material that is poorly wetted by the polymer, allows obtaining polymer blocks and removing them from the matrix. In addition, a massive multi-bed matrix creates the same temperature conditions in a polymerization furnace for all objects.

Claims (2)

The use of the proposed device preparation facilities for. Ultramicrotomes reduce the complexity of the preparation of objects and virtually eliminate the mechanical sharpening of the pyramid, and hence the need for devices such as TM-BS. Claim 1 ,. A device for preparing volumes for ultramicrotomes containing a matrix, characterized in that, in order to increase convenience, in the work of productivity and quality of preparation of objects, the matrix is made of two parts, made in the form of disks, the bottom of which is made with recesses with a transparent bottom, and the upper one is made with holes coaxially indented. 2. The device according to A.1, which means that the disks are 6 ° C low adhesion from the material to the polymer. 3. The device according to claim 1, about t l and so that the upper disk is made of elastic material. Sources of information taken into account in the examination 1. The prospectus of the company Reichert of the device TM-BS. Trimmvowich tung Zum Ultramikroton Reichert Auctrla I Ttt-60 VI-11D8 / 68. 2. Journal of Sweden LKB-devices, If 2, August 1978, p .- 3.