SU902462A1 - Method for cultivating yeast - Google Patents

Method for cultivating yeast Download PDF

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Publication number
SU902462A1
SU902462A1 SU802900808A SU2900808A SU902462A1 SU 902462 A1 SU902462 A1 SU 902462A1 SU 802900808 A SU802900808 A SU 802900808A SU 2900808 A SU2900808 A SU 2900808A SU 902462 A1 SU902462 A1 SU 902462A1
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SU
USSR - Soviet Union
Prior art keywords
yeast
cultivating yeast
erg
intensity
cultivating
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SU802900808A
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Russian (ru)
Inventor
Ш.А. Абрамов
С.Ц. Котенко
А.Т. Маммаев
В.И. Рыбникова
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Отдел Биологии Дагестанского Филиала Ан Ссср
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Priority to SU802900808A priority Critical patent/SU902462A1/en
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Publication of SU902462A1 publication Critical patent/SU902462A1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Description

троле 6,79 ± 0,42 млрд. (точность опыта 3,5-4%).Trol 6.79 ± 0.42 billion (accuracy of the experiment 3.5-4%).

Таким образом, лазерный свет интенсив-, ностью 1,5-10 эрг/см -с стимулирует процесс размножени  дрожжей в аэробных услови х более, чем в три раза.Thus, laser light with an intensity of 1.5–10 erg / cm – s stimulates the multiplication of yeast under aerobic conditions by more than three times.

Пример 2. Дрожжевую суспензию, приготовленную как в примере 1, облучают азотным лазером типа ЛГИ-2 (длинаExample 2. A yeast suspension prepared as in Example 1 was irradiated with an LGI-2 nitrogen laser (length

оabout

волны 3370 А, интенсивностью 1,5-10 эрг/ /см2-с) в течение 8-10-э с при 18°С.waves 3370 A, intensity 1.5-10 erg / / cm2-s) during 8-10-e s at 18 ° C.

Взвесь дрожжевых клеток в объеме 1 мл с концентр ащи.ей 100 тыс. в 1 мг помещают в кварцевую кювету и облучают при посто нном перемешивании. После облучени  производ т посев дрожжевой суспензии в виноградное сусло в скл нки Эрленмейера.A suspension of yeast cells in a volume of 1 ml with a concentrate of 100 thousand 1 mg is placed in a quartz cuvette and irradiated with constant stirring. After irradiation, yeast suspension is sown in a grape mash in Erlenmeyer tanks.

Дл  определени  процесса интенсификации размножени  дрожжей через каждые 12 ч подсчитывают количество дрожжевых клеток в 2 мл в камере Гор ева. Результаты исследований представлены кривыми роста на чертеже, из которого видно, что лазерный свет стимулирует процесс размножени  дрожжей в анаэробных услови х более чем в 2,8 раза. В опыте максимальное количество клеток в 1 мл достигает 170 млн, в контроле 60 млн.To determine the process of intensifying the multiplication of yeast, every 12 hours, the number of yeast cells per 2 ml is counted in the Gorev chamber. The research results are represented by growth curves in the drawing, from which it is seen that laser light stimulates the process of yeast multiplication under anaerobic conditions by more than 2.8 times. In the experiment, the maximum number of cells in 1 ml reaches 170 million, in the control 60 million.

Таким образом облучение лазерным свеоThus, laser irradiation

том с длиной волны 337 А, и интенсивностью 1,5-10 эрг/см2-с позвол ет увеличить выход дрожжей в 2,8-3,0 раза по сравнению с известным способом.A volume with a wavelength of 337 A and an intensity of 1.5-10 erg / cm2-s allows an increase in the yield of yeast by 2.8-3.0 times as compared with the known method.

Claims (3)

1. Авторское свидетельство СССР № 552353, кл. С 12В 1/22, 1975.1. USSR author's certificate No. 552353, cl. C 12B 1/22, 1975. 2. Авторское свидетельство СССР по за вке № 2739205/28-13, кл. С 12G 1/02, 1979.2. USSR author's certificate in application number 2739205 / 28-13, cl. C 12G 1/02, 1979. 3. Авторское свидетельство СССР № 276887, кл. С 12С 11/08, 1968.3. USSR author's certificate No. 276887, cl. From 12C 11/08, 1968.
SU802900808A 1980-03-26 1980-03-26 Method for cultivating yeast SU902462A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002062984A1 (en) * 2001-02-08 2002-08-15 Six Forest Bio-Science Institute Limited The yeast with immunoregulation activity to the k cell as well as the production method and the use thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002062984A1 (en) * 2001-02-08 2002-08-15 Six Forest Bio-Science Institute Limited The yeast with immunoregulation activity to the k cell as well as the production method and the use thereof

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