SU895364A1 - Plant grafting method - Google Patents

Description

(5) METHOD OF PLANT PLANTING

I

The invention relates to rural and forestry.

There is a known method of grafting plants, which includes the treatment of copulation slices with a solution of kinetin 1.

There is a known method of grafting plants, which involves treating copulation slices with solutions of a plant growth stimulator, for example, auxin 2,

The disadvantage of these methods is that the effectiveness of the rotactivating substances is low in cases of inoculations of weakly compatible components.

The purpose of the invention is to increase the survival rate of poorly compatible vaccination components.

The goal is achieved by the use of 0.008-0, an aqueous solution of dimethylsulfoxide (DMSO), as a growth stimulator solution in the treatment of sections;

Below are examples of the implementation of the method.

Laboratory field experiments and field experiments were carried out using the example of 0 species of woody plants belonging to 22 genera from 6 families; As a stock, 22 species were tested, and 26 species were given as a privo, for a total of 7 thousand injections of vaccinations in the amount of 0 thousand pieces. Weakly compatible vaccine components were selected.

In most cases, experienced. In variants there was a powerful and abundant callus formation, high-quality and fast intergrowth of components, an increase in survival rate by an average of 30-801 compared with the control (without treatment).

The results of the experiments were judged at the end of the vegetation period by the yield of standard seedlings, and the quality of the intergrowth of the components was evaluated by the anatomical picture of sections of the intergrowth sites.

Example 1 When inoculated with koi spraying after cutting cuttings. cherries, ox-eye, Kitaevska black and Renklod drain, Ugorka, their lower sections were moistened with 0, DMSO solution. The sections on the rootstock were treated in the same way - respectively, ordinary cherry, cherry plum, turn. After processing, the stalk was connected to the stock, the grafting site was tightly wrapped with a PVC tape to ensure the density of the contact between the graft and the graft and create an isolated medium at the grafting site. The upper cut of the cutting was smeared with pitch or plasticine and closed with a grafted cork with a hermetic bag. The bag below was wrapped around, creating a favorable climate around the cutting and grafting site. Grafts without DMSO solution were used as controls. Survival rate reached against in control (without treatment). In one year, more than twice the yield of standard seedlings was obtained. Example 2. Slices of cuttings of oak oak, dubolabotny, Scarlet oak and sections of rootstock (p oak boreal moistened with 0, DMSO solution, and then instilled by copulation according to the method described above. In this experiment, the grafting of treated grafts treated

Efficiency of accretion in grafts (grape varieties Aligate and stock copta grapes Ripari x X Rupestris 101-14) prepared for vaccination according to the proposed and known methods

No. p / p

Experience options

Control (water treatment) 28.6 ± 2.9

Processing of sections with a 0.005% gibberellin solution (known method) 35, It4.4

Treatment of sections with dimethyl sulfoxide solution with concentration,%:

T a b l

and c

 accretion 98 versus 35% in control (without treatment). The application of the method makes it possible for a short period of time (3 years) to obtain fruit bearing individuals of the named North American oak species, which are in the country of the unit, and to collect seeds for their mass reproduction. These types of oak trees are not demanding on soils and are of great value for afforestation and planting of populated places in the European part of the USSR. In addition, their seeds have high fodder qualities (for example, marsh oak acorns contain: 1 protein, S%, 28.7% fat, carbohydrates 8.21; for comparison, oak acorns contain: 5 protein,% , fat - 6,%, carbohydrates - 4.3%). Example 3. Slices of cuttings of walnut and slices of the stock - walnut black moistened 0 ,, DMSO solution. Then the cutting was connected to the stock and the junction was tightly wrapped with a tape of PVC film (in all experiments, vaccinations were performed according to the standard procedure). In the above experiment, 95% of vaccinations against 35% in the control (without treatment) survived. Example 4. The proposed method was compared with the methods of preparing plants for grafting by treating copulation sections with auxin, kinetin, or gibberellin solutions. The results are shown in table 1-3.

589536 6

/ n Variants of experience I% intergrowth

0,00136,1t6,1

0.00539.7i2.0

0.008ii6.6 ± 3.10, 145.7t1.7

0.15i48.0t, 3

0.2536, Oil, 6

The effectiveness of accretion in a graft (walnut) and stock (black walnut) prepared for inoculation according to the proposed and known methods.

-.

/ Variants of experience% intergrowth

1 Control (water treatment) §1,012,7

Processing slices 0, the solution of kinetin 46,115,2

Treatment of sections with dimethyl sulfoxide solution with concentration,%:

0,00150,017,6

0,00556,316, if

0,00886,0li, 8

0,19,8t, 6

0.1579.3t2.8

0,2548,015,5

Efficiency of accretion

(pine), prepared for vaccination

on the proposed and known methods

No. p / p

Experience options

Control (water treatment) 90.0 ± 1.2

Processing sections of 0.00i-nym

auxin solution (known

method) -92,311,6

Continued table. one

t.

table 2

Table 3

% accretion

Claims (2)

1. Turkish R.Kh. Instructions for the use of stimulators are sufficient for vegetative propagation of plants. M., USSR Academy of Sciences, 1962, p.71. 2. Turkish R.Kh., Polikarpova F.Ya. Vegetative reproduction of plants with the use of growth stimuli. M., Science 1968, p.93 (prototype).