SU862878A1 - Method of preserving biological tissues - Google Patents

Description

(54) METHOD OF CONSERVATION OF BIOLOGICAL TISSUES

one ,

This invention relates to medicine and may find application in traumatology, surgery and transplantology.

There is a known method of preserving biological tissues by placing them in a liquid preservative medium flj.

However, the terms of conservation in a known manner are short.

The aim of the invention is to lengthen the terms of conservation,

This goal is achieved by the fact that the method of the provision of biological tissues is carried out by psalegdene into liquid preserving media, the graft is maintained at a temperature of 2-4 ° C in 0.25 g, 5% solution of glutaral algebra prepared on Ringer's solution. -Lokka at pH 7.3-7.5,

Conservation of bone, carpal grafts, tendons, vessels, and fasciae are carried out with a 25-2.5% solution of glutaraldehyde with a pH of 7.3-7.5 at a temperature. Preservation is carried out for 1 year. These grafts have pronounced bacteriostatic activity. Microbiological studies show that glutaraldehyde solutions (0.25-2.5%) have a bacteriostatic effect on the microbial flora, both resistant and sensitive to the most widely used antibiotics and antiseptic solutions. The degree of this bacteriostatic effect depends on the type of microorganisms, the duration of their contact with the preparation, the concentration of the latter, the density of the bacterial populations.

The results of the conducted studies indicate the possibility of harvesting biological tissues in non-sterile conditions, since

15, the preservation process in solutions of Glutaric aldehyde causes the death of microorganisms: in a 2.5% solution in 1-2 days, in a 0.2m solution in 10-15 days.

20 Studies of the bacteriostatic properties of canned bones by the proposed method show that in the immediate vicinity of the washed graft is the ability

25 bacteria multiplication is suppressed. The obtained data allow us to conclude about the resistance not only of autono and allo- and xeno-tissues, preserved in glutaraldehyde, to

30 superinfection at the time of the transplant. it

indicates the possibility of their use in conditions of an infected wound.

The grafts are taken 1-3 hours after the animals are taken from the animals, rabbits) under non-sterile conditions, they are washed in a physiological solution, cleaned of soft tissues, preserving the integrity of the periosteum. Then the plastic material is immersed in a pre-prepared solution of glutaraldehyde. Grafts are kept in hermetically sealed glass containers in a domestic refrigerator at a temperature of 2-4 ° C. After 15-2 U days, the graft becomes sterile and its antigenic activity is suppressed while preserving its biological properties. Since then, the graft can be used to replace bone defects in an infected wound for 1 year.

Experimental studies of the plastics of residual purulent bone cavities provide positive results. By histological methods, it has been found that these grafts have high osteoplastic properties, and there is no pronounced immunological response.

The glutaraldehyde solution is prepared by adding the required amount of concentrated reagent to the sterile saline.

The proposed method of preservation provides long-term storage of allogeneic tissues with the preservation of their biological properties. Transplanted grafts allow restoring the morphological structure of the operated object and function, and the feasibility of using them in an infected wound is shown.

Claims (1)

Invention Formula The method of preserving biological tissues by placing them in a liquid preserving medium, differs in that, in order to extend the terms of preservation, the graft maintained at a temperature of 2-4 ° C. O, 25-2.5% solution of glutaraldehyde prepared. In Ringer-Locke solution at pH 7.3-7.5, Sources of information taken into account in the examination 1, Parfenyeva V, F ,, Rozvadovsky v, D. and Dmitrienko V, I, Conservation of homologous bone grafts, Chisinau, Medicine, 1369, с, 10-20,