SU859889A1 - Method of 1-methyl-4-(n-methylpyrrolidil-1)- -carbolene hydrochloride quantitative determination - Google Patents

Description

1 ml of the resulting composition was added 9 ml of distributive water and 3 drops of concentrated nitric acid, stirred and fluoridated with respect to a standard aqueous solution containing 5 mcc / ml of Brevicollin hydrochloride, 10 ml of which was filled with 3 drops of concentrated nitric acid, and fluoridated simultaneously but with test solutions. The results of determination of brevicolline hydrochloride in a 1% solution are presented in Table. 1. Example 2. Determination of brevicolline hydrochloride in blood and urine. To 1 ml of blood plasma or 1 ml of urine, add 2 drops of OD of sodium hydroxide solution and 10 ml of ether and extract them for 2 minutes. The ether layer is drunk in a evaporation cup. Extraction is repeated twice. The ether is distilled in a water bath. To the residue was added 10 ml of distilled water, 3 batches of conentric nitric acid, stirred and fluoridated using standard solutions with a content of 0.5: 1: 3 or 5 µg / ml brevicollin hydrochloride. To this end, 3 drops of concentrated nitric acid are added to 1O ml of 894 standard solution and fluoridated simultaneously with the test solutions. In artificial mixtures, with a content of more than OD µg / ml of brevicolline hydrochloride, si is determined completely (100%). The fluorescence excitation spectrum of the solution obtained is in the region of 28O-430 nm with a maximum of 380 nm, and the fluorescence emission spectrum is in the region of 400-520 nm with a maximum of 44O nm. The linear relationship between fluorescence intensity and the concentration of brevicolline hydrochloride is from 0, O1 to 8 µg / ml (Tables 2 and 3). The fluorescence intensity does not change for 2 hours. In the dosage form, the concomitant ingredients do not affect the fluorescence intensity. With a content of 0.1 µg / mp, brevicolline hydrochloride in the blood or urine is completely cleared (1OO%), i.e. blood and urine components do not affect the fluorescence intensity. The sensitivity of the known method of 0.5 µg / ml, proposed - Oh, 01kmg-ml.

Fluusimetric determination of brevicolline hydrochloride in 1% - ом om solution (for the study taken 0.3 ml of 1% solution).

0,0098 О, О098 О, О1ОО О, О098 О, ОО96 0, ОО96 О, 0098 О, ОО98 0, ОО96 О, ОО98

Table I

0, O097 2. U

.-

Ilo

.-four

0, GO97 Ila

8598896

Investigating the inhibition of fluorescence of brevicopline hydrochloride (µg / ml) og concentration, azoma acid,

The number of drops of concentrated nitric acid

I

2;

3 4

5 The dependence of the fluorescence intensity on the solution of nitric acid

table 2

Relative fluorescence intensity

62 85 90 90 76 t Table 3 Concentrations of brevacolline hydrochloride.

Claims (1)

Claim Method for the quantitative determination of 1-methyl-4- (Ν-methylpyrrolidyl-1) - (1 -.-Capsules with hydrochloride adapting to the test sample by a chemical reagent, characterized in that, in order to increase the sensitivity and selectivity of determination, a nitrogen reagent is used as a chemical reagent