SU843908A1 - Stimulant for formation of leguminous crops nodules - Google Patents

Description

This invention relates to microbiology and concerns the use of tris- (2-hydroxyethyl) ammonium salt of phenoxyacetic acid in agriculture as a stimulant for the fixation of molecular nitrogen by leguminous plants.

The use of indole acetic acid as a stimulator of nodule formation in legumes II is known.

However, the activity of indole acetic acid as a stimulator of the formation of nodules in leguminous plants is insufficient.

The purpose of the invention is to increase the activity of substances used to form nodules in legumes.

This goal is achieved by using tris- (2-hydroxyethyl) ammonium salt of a halogen-substituted phenoxyacetic acid of the general formula X CfiHu-nOCHaCOO. HN (CH2CH2OH) 3 where X-C1, Br;

, 3,

as a stimulator of the formation of nodules in legumes.

Previously, it was known to use tris (2-hydroxyethyl) ammonium salt of a halogen-substituted phenoxyacetic acid as a stimulant of mulberry and oak silkworm productivity {21.

The starting halogen-containing phenoxyacetic acid is mixed with triethanolamine in equimolar amounts in a polar (ethanol, acetone) or non-polar (benzene) solvent. The reaction mixture is heated with stirring until the acid is dissolved. The precipitate of tris- (2-hydroxyethyl) ammonium salt of the phenoxyacetic acid, which had precipitated after cooling, was washed with diethyl ether and dried in a water-jet pump vacuum. The yields of tris- (2-hydroxyethyl) ammonium salts, their melting points and elemental analysis data are given in Table. 1. Compounds of general formula

15

-OSNgSOO NN (CH2CHNON) s

X

Table I

Example. Pre-germinated sterile seeds of meadow clover (Trifolium pratense, L) were germinated on Knop agar medium. On Wednesday made a three-day culture of nodule bacteria Rhizobium trifolii, strain 159, at the rate of 1 million cells per 1 ml of medium. Simultaneously with the introduction of the germinated seeds, the test substances were added to the medium. Inoculated plants of clover bz phytohormone supplements served as controls. Plants were grown for two months, after which they took into account the number of nodules on the roots of the plants, and also measured the length of the roots and the height of the land part of the plants.

The results of the experiments (five-fold repetition), statistically processed according to Plokhinsky, are presented in Table. 2

The number of nodules on the root x of the clover of the meadow, inoculated with nodule

bacteria Rhizobium trifolii, with additional processing / OCH2SOO. HN (CH., CH20Ii) 3

The high positive effect, along with the availability of the proposed compounds, makes it possible to recommend them for use in agriculture in order to increase the efficiency of legume-rhizobial symbiosis. In the case of presowing treatment of seeds, the consumption of such compounds does not exceed 1 g per 0.5 million hectares of sowing.

Claims (1)

Invention Formula Use of tris- (2-hydroxyethyl) ammonium sun; a halo-substituted phenoxy-acetic acid; with the general formula NM (CH2CHYUN); X SbNz-IOSNgSOO where X is C1, Br; n 1.3. T a b .4 and c a 2 X