SU825078A1 - Method of removing metabolism products from tissues of organism - Google Patents

Description

The invention relates to medicine and concerns methods of detoxifying the body, namely the removal of toxic products from tissues.

There is a method of removing metabolic products from body tissues by intravenous administration of medicinal solutions 1.

However, the known method does not allow removal of toxic products through the lymphatic system.

The purpose of the invention is the removal of toxic products through the lymphatic system.

This goal is achieved by the fact that the method of removing metabolic products from body tissues is carried out by intravenous administration of medicinal solutions, a 10-30% solution of mannitol is introduced as a medicinal solution at a rate of 60-80 drops per minute, in the amount of 1-2 g / kg body weight.

Example 1. (Control). The dog is fixed on onepaTj, HOHHOM table on the back. A medication of 7.0 ml of a 2% omnopone solution is performed. Under intubation ether anesthesia, the femoral vessels are opened and cannulated. Drainage of the thoracic duct is also performed. The initial level of lymphatic drainage is 0.2 ml / min. Blood pressure 130 mm Hg Lymphatic sampling is performed, in which background toxicity is determined by placing a biological test on mice with a blocked reticuloendothelial mascara system. Pelican. The toxicity of the initial portion of lymph is equal to 0 units. By bleeding blood pressure is reduced to 40 mm Hg. and maintained at this level for 1 hour. At the end of the hypothesis period, the rate of lymphatic outflow is 0.4 ml / min. The toxicity of lymph in the same period is equal to O-1 units. Additional bloodletting was performed. Blood pressure About mm Hg. Stop artificial ventilation of the lungs. There comes a cardiac arrest. Clinical death for 1 min, followed by recovery by reinfusing the released blood, indirect heart massage, and artificial ventilation of the lungs. During the recovery period: the arterial pressure is 100 mm Hg, the lymph outflow from the drained thoracic duct rises sharply to 1.5 ml / min, and the lymph toxicity is 1-2 units. 30 minutes after clinical death, the rate of lymphatic drainage is 0.6 ml / min, and the toxicity of lymph is 1 unit. A total of 7 such experiments were conducted. Example 2. A dog is fixed on an operationally .1 table in a position on the back. Promedication - 10.0 ml of 2% omnopone solution. Under intubation ether anesthesia, the femoral vessels are opened and cannulated, and the chest flow is drained. The lymphatic drainage level is 0.2 ml / min. D oral pressure is 100 MmHg. Lymphatic sampling is performed, in which background toxicity is determined by placing a biological sample on men with a blocked reticuloendogelium mascara system, Pelikan. The toxicity of the initial portion of lymph is equal to O units. By bleeding blood pressure is reduced to 40 mm Hg. and maintained at this level for 1 hour. At the end of the hypothesis period, the rate of lymphatic outflow is 0.3 ml / min, and its toxicity is 1-2 units. Additional bloodletting was performed. Blood pressure About mm Hg. Art. Artificial lung ventilation is stopped, cardiac arrest occurs. Clinical death for 1 min, followed by revitalization of the re-infusion of released blood, indirect heart massage and mechanical ventilation of the lungs. During the recovery period, the arterial pressure rises to 130 mm Hg, the lymphatic drainage rises to 0.8 ml / min, and the toxicity of the lymph is 2 units. After 30 min after clinical death (lymphatic drainage 0.6 ml / min, lymph toxicity 2 units) intravenously at a speed of 60 cap. In 1 minute, 200 ml of a 30% mannitol solution is injected. 15 minutes after the infusion of the drug, the rate of lymph outflow increases to 1.2 ml / min. Lymph toxicity is 4 units. Similar results were obtained in 6 identical experiments. Example 3. The dog is fixed on the operating table in position on the back. Under intravenous thiopental anesthesia (20 ml of a 5% solution), the thoracic duct is drained. Every 20 minutes of observation, the obtained lymph for toxicity was studied by placing a biological sample on the mice with the reticuloendothelial system of mascara blocked by Pelikan. During the first hour of observation, lymph toxicity is O units. At 60 minutes, the animal is injected intravenously at a rate of 60 drops / min. 300 ml of a 30 ° / full solution of mannitol is administered. In the next 2 hours of observation, the toxicity of the lymph does not change and amounts to O units. Total produced 4 such experiments. Example 4. The dog is fixed on the operating table in position on the back. Promedication and induction anesthesia - a 2% solution of promedol 5.0 ml and a 5% solution of sodium thiopental. Under intubation ether-oxygen anesthesia, femoral vessels are isolated and cannulated, the thoracic duct is drained, and thoracotomy is performed. In order to simulate the process of endogenous intoxication, conditions of acute cardiovascular insufficiency are created by compressing the heart with a rubber balloon inserted into the pericardial cavity. At the same time, the arterial pressure decreases to 60–40 mm Hg. Lymphatic sampling is periodically carried out to determine toxicity by placing a biological sample in mice with a blocked reticuloendothelial system with a Pelican duck. A number of physiological parameters are also measured: pulse, blood pressure-, central venous pressure, cardiac output, electrocardiogram. Subsequently, with stable hemodynamic parameters, the animal is clogged. Similar data were obtained in 8 identical experiments. The table presents the results of modeling endogenous intoxication under the creation of conditions that hinder the work of the heart, with subsequent tissue detoxification by injecting a solution of lmannitol. Example 5. A dog is fixed on the operating table in an ovine on the back. Under intravenous hexenal anesthesia (0.03 g / kg), thoracic cannulation, laparotomy, clamping of the mesenteric artery trunk are performed. Periodically, lymph and blood plasma are collected and their toxicity is determined by placing a biological sample on the muscles with the reticuloendothelial system blocked with the "Pelican" duck. Determining the toxicity of lymph and blood plasma, taken before the mesenteric artery was occluded (control), showed a weakly positive reaction (2–4%). Some toxicity is due to anesthesia, animal fixation and operative trauma. Occlusion of the mesenteric artery caused the apparent toxicity of lymph (25%) and plasma (23%). For tissue detoxification, a 10% solution of mannitol (60 caps per 1 minute) was administered intravenously at a rate of 10 ml / kg. This led to a sharp increase in lymphatic toxicity (480%) and its decrease in blood plasma (7%). The proposed method effectively removes toxic products through the lymphatic system, which significantly speeds up the process of curing patients and reduces the cost of treatment.

Claims (1)

Invention Formula A method of removing metabolic products from body tissues by intravenous administration of medicinal solutions, characterized in that, in order to remove toxic products through the lymphatic system, as a decariate solution a 10-30% solution of mannitol is introduced dropwise at a rate of 60-80 drops per minute, in the amount of 1-2 g / kg body weight. Sources of information taken into account in the examination 1. Resuscitation. Ed. G. N. Tsybul, ed. 2, M., “Medicine, 1976, p. 218.