SU959139A1 - Method of simulating clinical death and postreanimation desease - Google Patents

Description

(54) METHOD FOR MODELING CLINICAL DEATH AND CONSTRUCTION DISEASE

one

The invention relates to experimental medicine and can be used to study clinical death and post-resuscitation conditions.

There is a method of modeling clinical death and postresuscitation disease by completely stopping blood circulation by blood loss from the abdominal aorta, followed by its restoration and revitalization of the animal 1.

However, the implementation of the known method, which is difficult and time-consuming in the technical implementation, causes bleeding in the post-resuscitation period from the injured aorta, which distorts the picture of the post-resuscitation period by trauma to the organs of the cavity and inflammatory changes. The duration and ambiguity in time of the dying period worsens standardization and reproducibility of experiments.

The aim of the invention is to approximate the model to the clinical course of pathology.

The goal is achieved by the fact that in the implementation of the method of modeling

clinical death and postresuscitation disease by completely stopping the blood circulation with its subsequent restoration and revitalization of the animal, the distinguishing feature is that the complete blood circulation is stopped by intravenous injection of acetylcholine solution in 0.1-0.2 ml of physiological diluent.

The method is carried out as follows, Q, time.

A solution of acetylcholine chloride in a dose of 5-15 mg per 100 g of animal weight in a volume of 0.1-0.2 ml of physiological saline is injected into the femoral vein. After 3-5 seconds, full cardiac arrest occurs.

15 and at the same time at the same time, the blood pressure in the aortic graft drops to zero. The moment of cardiac arrest can be considered the beginning of clinical death. After 5–15 minutes after the circulatory and respiratory arrest (the time of clinical death), the animal begins to undergo an indirect heart massage and artificial ventilation of the lungs until it recovers stable heart activity and self-sustaining

breathing. During the indicated time of clinical death at the onset of resuscitation, the fully administered dose of acetylcholine is destroyed by the influence of the active endogenous cholinesterase enzyme.

The effectiveness of the method was tested in experiments on 50 animals (white outbred rats of both sexes weighing 150-200 g). To control the onset of clinical death and complete cessation of blood circulation in the brain, in addition to the blood pressure in the abdominal aorta and electrocardiography, the brain's bioelectric activity was recorded. 15-25 seconds after the administration of acetylcholine, isoline was observed on the electroencephalogram.

With a 5-minute clinical death in the experiments, there were 20 animals, all of them were revived. After 8–10 minutes of death, 20 animals were revived. Of the 10 animals with 15 minutes of clinical death, 9 rats were revived. This proves that the revival of animals with this method of modeling clinical death is much higher than with all the others. At the same time, this method allows to significantly increase the duration of clinical death, which with other methods is significantly less and more often does not exceed 3-5 minutes.

In resuscitated animals, pronounced signs of post-resuscitation disease develop (especially after 10–15 minutes of death).

At the same time, this method allows to significantly increase the duration of clinical death.

This method is simple and easily reproducible in technical design, eliminates the trauma and bleeding, reduces the death of animals, allows a high degree of standardization

experience (period of dying and clinical death), which is very important for reproducibility of postresuscitation disease. In addition, the number of animals used decreases, the experimenters' time spent on reproducing decreases.

postresuscitation disease.

The advantage is that the method is designed for small animals and can be extended to other types of animals.

Claims (1)

Invention Formula A method of modeling clinical death and postresuscitation disease by completely stopping the blood circulation and then restoring it and reviving the animal, characterized in that, in order to bring the model closer to the clinical course of pathology, the blood circulation is completely stopped by intravenous injection of acetylcholine 0.1-0.2 ml saline solution. Sources of information taken into account in the examination 1. Proteins and enzymes in clinical and experimental studies. Scientific works № 126 Omsk State Medical Institute, 1977, Omsk, p. 3-13.