SU82318A1 - Method of preparation of dry dysenteric bacteriophage - Google Patents
Method of preparation of dry dysenteric bacteriophageInfo
- Publication number
- SU82318A1 SU82318A1 SU398805A SU398805A SU82318A1 SU 82318 A1 SU82318 A1 SU 82318A1 SU 398805 A SU398805 A SU 398805A SU 398805 A SU398805 A SU 398805A SU 82318 A1 SU82318 A1 SU 82318A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- dysenteric
- bacteriophage
- dry
- preparation
- agar
- Prior art date
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Description
Предметом изобретени вл етс метод приготовлени сухого дизентерийного бактериофага, употребл емого в медицинской практике.The subject of the invention is a method for preparing a dry dysenteric bacteriophage used in medical practice.
Предлагаемый метод отличаетс от известных тем, что дл выращивани бактериофага посев культур дизентерийных бактерий производ т во .ей толще сло полужидкого агара. П)игатовленные таким методом препараты биологически более активны, а себестоимость их, в результате экономии сырь , значительно снижаетс .The proposed method differs from the known ones in that for the cultivation of bacteriophage the seeding of cultures of dysenteric bacteria is made in a thicker layer of semi-liquid agar. O) drugs that are igatated by this method are more biologically active, and their cost, as a result of saving raw materials, is significantly reduced.
М сопептонный полужидкий агар, содержащий 0,5п агар-агара, при рН 7,4 разливают дозами по 10 л в бутыли емкостью 20 л и в автоклаве стерилизуют. Посев производ т одновременно культурами бактерий дизентерии Шига и Флекснера, смытыми с плотной среды, и лизирующими их маточными бактериофагами. После посева агар тпхательно смещивают вращательными движени ми баллона и на 18 час. помещают в термостат при температуре 37. Качество лизиса контролируют микроскопически и просмотром мазков. Обезвреживание массы производ т парами хлороформа при температуре 40 в течение 48 час. После этого к ней добавл ют 60% декстрина и 16% сыворотки. Далее, полученную массу тонким слоем нанос т на металлические листы и сущат при температуре 40 в течение 8-9 час. А затем ее соскабливают и делают посев дл проверки специфической стерильности, после чего титруют по Аппельману.M septonous semi-liquid agar containing 0.5p agar-agar, at pH 7.4, is poured in doses of 10 liters into bottles of 20 l and sterilized in an autoclave. Sowing was performed at the same time by cultures of Shiga and Flexner dysentery bacteria, washed out from a dense medium, and uterine bacteriophages lyticing them. After sowing, the agar is displaced with rotational movements of the cylinder and for 18 hours. placed in a thermostat at a temperature of 37. The quality of lysis is monitored microscopically and by viewing smears. Mass neutralization is carried out with chloroform vapor at a temperature of 40 within 48 hours. Thereafter, 60% dextrin and 16% serum are added to it. Further, the resulting mass is applied in a thin layer onto metal sheets and is formed at a temperature of 40 for 8-9 hours. And then it is scraped and sown to verify specific sterility, and then titrated according to Appellman.
При наличии удовлетворительных титров массу перемалывают, добавл ют к ней сахар и тальк и пускают на изготовление таблеток.When there are satisfactory titers, the mass is ground, sugar and talc are added to it and allowed to make tablets.
Полученные описанным способом таблетки подлежат обычному контролю.Obtained as described tablets are subject to normal control.
№ 82318- 2 -No. 82318-2 -
Г1 р е д м с т и 3 о б р е т е н и G1 re dmstat and 3 about b ete n i
Метод пригото1злени сухого дизентерийного бактериофага, о т л и;чающийс тем, что дл выращивани бактериофага посев культур дизентерийных бактерий производит во всей толще ело полужидкого агара.The method of preparing dry dysenteric bacteriophage, about t and t, is due to the fact that for the cultivation of bacteriophage seeding cultures of dysenteric bacteria produces in the entire thickness of the body of semi-liquid agar.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU398805A SU82318A1 (en) | 1949-04-30 | 1949-04-30 | Method of preparation of dry dysenteric bacteriophage |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU398805A SU82318A1 (en) | 1949-04-30 | 1949-04-30 | Method of preparation of dry dysenteric bacteriophage |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU1927037A Addition SU468365A2 (en) | 1973-06-04 | 1973-06-04 | Delay device |
Publications (1)
Publication Number | Publication Date |
---|---|
SU82318A1 true SU82318A1 (en) | 1975-05-25 |
Family
ID=20436633
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU398805A SU82318A1 (en) | 1949-04-30 | 1949-04-30 | Method of preparation of dry dysenteric bacteriophage |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU82318A1 (en) |
-
1949
- 1949-04-30 SU SU398805A patent/SU82318A1/en active
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