SU762821A1 - Culture medium for cultivating insects - Google Patents

Description

The invention relates to the field of agriculture, in particular to the means for the cultivation of beneficial insects. five

Known food for the cultivation of insects, containing wheat germ, casein (according to Hammersten), 2M solution of caustic potassium, sucrose, ascorbic acid, 10

p-hydroxybenzoic acid methyl ester, formaldehyde and distilled water [1].

However, this feed can only be used in the cultivation of a harmful bug.

’. Known nutrient medium for growing Agsha sy 5 Yuz, containing casein (according to Hammersten), 2M caustic potassium solution, sucrose, wheat germ 20, ascorbic acid, p-hydroxybenzoic acid methyl ester, formaldehyde 20%, distilled water £ 2].

The main disadvantage of this environment 25 is that it applies only to Agsha siz Yu5.

The purpose of the invention is the expansion of the range of nutrient media for insects.

The goal is achieved by the addition of cryopreserved butterflies and fresh yolk of chicken eggs, the components are taken in the following ratio, wt.%:

Cryopreserved butterflies

Casein (according to Hammersten)

2M potassium hydroxide solution

Sucrose

Fresh Chicken Egg Yolk

Wheat embryos Ascorbic acid Methyl ester of p-hydroxybenzoic

35.0-45.0

9.0-11.0

2.5-3.5

4.0-6.0

4.0-6.0

2.0-4.0

0.15-0.25

acid 0.15-0.25

formaldehyde 20% 0.23-0.25

Distilled

water 'the rest.

Cryopreserved Butterflies Seedotrogs are waste

trichogram production.

Butterflies sitographers after their windows-;

use on biofabs ¹

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In the case of industrial dilutions, trichograms are cleared of debris and subjected to cryopreservation according to the method of Rudnev et al. [3]. Cryopreserved butterflies stored in liquid nitrogen at -196 ° C until use. As the butterflies need, the strainers are removed from the refrigerant, defrosted in air at room temperature, crushed in a porcelain mortar and introduced into the nutrient medium.

Wednesday is prepared as follows.

A portion of casein (according to Hammersten) is moistened in 25 ml of distilled water and hydrolyzed with 2M KOH on boiling4

water bath after 6, .h.

The mixture is cooled to 45-50 ° C and when mixed, sucrose, wheat germ, p-hydroxybenoic acid methyl ester, formaldehyde, e powder from cryopreserved butterflies and fresh egg yolk are injected. Last but not least. ascorbic acid is added to the mixture. The total amount of medium is adjusted, β Up to 100 g by adding the missing amount of distilled water.

The influence of the composition of the proposed medium on the growth and development of carnivorous bugs in comparison with the known medium is presented in the table.

The influence of the composition of the proposed environment on the growth and development of predatory

bedbugs compared to the famous

The composition of the nutrient medium The basis of nutrient media amount basics in environment g / 100 g The output of imago,% Duration of development LEE “chinok, days. The average weight of bedbugs per day, wingings, mg females the males Famous* Lyophilisates 25 79.4 32.7 51.0 49.3 ку ку ку willow chops thirty 91.2 28,8 62.0 52,8 Moths 35 89.6 28.2 61,8 51.9 Agta Siz ΐοε P According to Cryoconser 35 72.0 27.0 58,6 49.9 to the invention vored - ^y ' • - - ⁴ butterflies 40 88.2 25.8 68.1 55,6 ..Л-. · .. ·;: .-. ·.; · torogers - ' • 45 96.0 25.3 70.3 56.1 '  · -....... Ros) (ziz that with and 2 ΐ ν e η ί g ϊ h 5 and y According to Cryoconser 35 80.2 23.1 52.7 41.2 to the invention vored butterflies 40 92.3 20.0 60) 6 50.0 torogers - - - 45 ' 90.9 21.1 61.3 49.4

Presents data on the cultivation of predatory bugs Agta siz 1oz G.

From the data presented in the table, it follows; What is the use of the nutrient medium of the proposed composition allows you to grow all the feeding age of bedbugs Agta siz £ oz and ΓοόI ziz tas? ί νβπ ίг! з, from the second larval age to the imago; The developed medium fully satisfies the basic requirements of predators and ensures their normal viability. __

Breeding of Chytnykh Bugs on the proposed medium is 3 times cheaper than on the well-known feed. 65

The use of the nutrient medium according to the invention for feeding predatory bedbugs Agta sig 1o s, Ροάίβιΐδ tasi? Ϊ́νepΐgϊz provides the following advantages: the accumulation of predators in biofactories and in industrial laboratories for breeding during the year to create the necessary stocks, increasing the productivity of biofactories for entomophagous plants minimum twice due to the year-round cycle of their mass breeding ·, the release of predatory bugs on the field just in time, most762821

more vulnerable to pests of agricultural crops, which will significantly increase the efficiency of the use of entomophages in the biological protection of the crop from harmful harmful insect species.

The introduction of the proposed composition of artificial nutrient medium in biofactories will provide an opportunity to mechanize the process of cultivation of predatory bugs.

Claims (1)

Claim Nutrient medium for the cultivation of insects, mainly Agta SizUz, RoBN 5 from tasii ϊ νβη 1g 5 5, containing casein (according to Hammersten) 2M solution of caustic potassium, sucrose, wheat germ, ascorbic acid, p-hydroxybenzoic acid methyl ester, formaldehyde 20%, distilled water, characterized in that, in order to expand the range of nutrient media, it is additionally injected 25 waste of trichogram production (cryopreserved butterflies sitographers) and fresh yolk of chicken eggs, and the components are taken at the following ratio, wt.%: Cryoconverting butterflies 35.0-45.0 Fresh yolk irino go eggs ί 4.0-6.0 Casein (by ^: Hammerste Well) 9.0-11.0 2M potassium hydroxide solution 2.5-3.5 Sucrose 4.0-6.0 Wheat germ 2.0-4.0 Vitamin C 0.15-0.25 Methyl ester p-ok- sibenzoic acid 0.15-0.25 Formaldehyde 20% 0.23-0.25 Distilled water Rest.