SU739088A1 - Apparatus for culturing microorganisms - Google Patents

Description

(54) APPARATUS FOR CULTIVATION OF MICROORGANISMS

one

This invention relates to NWKPO biological purity and can be used to strain microorganisms, such as fodder yeast, on hydrolysis media and petroleum hydrocarbons.

The proposed apparatus is closest in design to the apparatus dp alrai of the microorganisms, made in the form of a rectangular container with a cap, the volume of which is divided by vertical heat exchange partitions into communicating and circulating cells in the upper and lower parts of the apparatus. tubular collectors for supplying air to the bubbling cells, branch pipes for introducing nutrient medium and ampoules with seeding by microorganisms and withdrawing the suspension of microorganisms, located on opposite apparatus, and foaming agents 1.

Such an apparatus cannot work in optimal growth modes, without continuous seeding of microorganisms together with a nutrient medium.

The aim of the invention is to increase the specific productivity of the apparatus.

For this, in the proposed culture, the length of each subsequent partition culture liquid exceeds the length of the previous partition, while the lower edges of the partitions are arranged in steps to form nutrient medium supply zones in the lower part of the tank, with tubular coltographs placed in bubbling cells at the level of the lower edge of the partition.

FIG. 1 schematically shows the proposed apparatus, vertical

15 cut; in fig. 2 - the same, view of the top with the removed cover; in fig. 3 and embodiments of the apparatus.

The device is made in the form of a tank 20, 1 with a lid 2, divided along the length by vertical heat exchange partitions 3 into sections at the top and bottom, consisting of bubble 4 and circulation 5 cells of straight coal section, separated by partitions 6. The length of partitions 3 and 6 increases stepwise as the culture fluid moves. The upper edges of the partitions can be placed at the same level.

In the lower part of the apparatus under pipe 4, tubular collectors 7 are installed with bubblers 8 for air supply. The cutoff level of these bubblers8 in each section is located at the lower end of the partitions 6. The collectors 7 are connected to a common distributing collector 9.

The apparatus is equipped with nozzles 10 for supplying a nutrient medium, a nozzle 11 for withdrawing a suspension of microorganisms, an nozzle 12 for air outlet and a nozzle 13 for draining the liquid.

Mechanical defoamers are installed on the lid of the apparatus (not shown in the figures).

The apparatus operates as follows. Air continuously flowing from the collector 7 through bubblers 8 into cells 4, barbates through a layer of liquid in them and is removed from the apparatus through nozzle 12. As a result of air supply to cells 4, intensive circulation of the yeast suspension occurs around the walls b, shown by arrows in FIG.

Moreover, since in the first section the sections of the bubblers 8 and the partitions 6 are raised high above the bottom of the apparatus, this circulation does not reach the bottom and a layer of monolithic liquid not containing microorganisms remains below. The thickness of this layer decreases when moving from section to section in accordance with the change of position of the partitions and bubblers,

The nutrient medium through the nozzles 10 enters the dead zone of the first section and, according to the law of communicating vessels, uniformly pacrtdels into each section, i.e. rtpo there is a parallel powering of sections on the substrate.

The step arrangement of the partitions 3 does not allow the yeast suspension to pass under their lower edges, so the suspension flows into the next section only from above. Thus, the first section works as if on its own and serves to increase the biomass of microorganisms; necessary for seeding the second section of the apparatus. The same applies to the remaining sections. From the first section, the suspension of microorganisms with a concentration of natural growth for the given conditions of cultivation flow

jBO second, etc. before exiting the apparatus. In this case, its total flow will increase stepwise. Such an organization of operation of the apparatus permits the abandonment of external seeding of microorganisms and the maintenance of an optimal mode of cultivation in each section.

. The temperature mode of growth is maintained by the heat transfer medium supplied to the partitions 3 and 6. To control the flow of the suspension flowing from one section to another, the upper ends of the partitions 3 can be located at different heights.

The advantages of the proposed apparatus are the possibility of rejecting the continuous seeding of the nutrient medium by microorganisms and the intensification of the growing process by maintaining an optimal mode in each section, which leads to an increase in its specific productivity.

Claims (1)

Invention Formula A device for growing microorganisms, made in the form of a tank with a lid, divided along the length by vertical heat exchange partitions, not communicating at the top and bottom of the section with bubbling and circulating cells, and equipped with tubular headers for supplying air to the bubbling cells, feeding pipes and output of a suspension of microorganisms located on opposite walls of the tank and mechanical defoamers, characterized in that, in order to increase the specific productivity apparatus length of each subsequent in the course of movement of the culture fluid of the partition, the separating section exceeds the length of the previous one, while the lower edges of the partitions are arranged in steps to form zones for supplying the nutrient medium in the lower part of the tank, with tubular collectors in the bubbling cells . Sources of information taken into account in the examination 1, Patent of England (1356860, class C 6 F, published 1974 (prototype) ft " -I  Phi1. / n A / f H // g / .2