SU731935A1 - Method for destructing cell membrane of chlorella - Google Patents
Method for destructing cell membrane of chlorella Download PDFInfo
- Publication number
- SU731935A1 SU731935A1 SU762426425A SU2426425A SU731935A1 SU 731935 A1 SU731935 A1 SU 731935A1 SU 762426425 A SU762426425 A SU 762426425A SU 2426425 A SU2426425 A SU 2426425A SU 731935 A1 SU731935 A1 SU 731935A1
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- SU
- USSR - Soviet Union
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- chlorella
- cell membrane
- destructing
- destructing cell
- hours
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
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Изобретение относитс к микроби .ологической промышленности и касаетс приготовлени белково-витаминных обогатителей кормов, используемых в сельском хоз йстве.The invention relates to the microbiological industry and concerns the preparation of protein-vitamin fortifiers for feed used in agriculture.
Известен способ приготовлени напитка из хлореллы, заключающийс в том, что свежую хлореллу суспендируют в воде, к суспензии добавл ют жидкую протеазу, продуцируемую микроорганизмами Bacillus natto, котора вызывает автолиз хлореллы l .A known method of preparing a chlorella beverage is that fresh chlorella is suspended in water, and a liquid protease produced by the microorganisms Bacillus natto is added to the suspension, which causes autolysis of chlorella l.
Недостатком известного способа вл етс невысокий выход белковых веществ.The disadvantage of this method is the low yield of protein substances.
Наиболее близким к изобретению решением вл етс способ разрушени клеточной оболочки хлореллы, предусматривающий тепловую обработку суспензии хлореллы, охлаждение ее с последующим ферментативным гидролизом 2 .The closest solution to the invention is a method for destruction of the chlorella cell wall, which involves the heat treatment of a suspension of chlorella, its cooling followed by enzymatic hydrolysis 2.
По этому способу гидролиз хлореллы провод т путем воздействи на оболочку цитолитическим ферментным препаратом, полученным из гриба Trichotheeium roseura.In this method, chlorella is hydrolyzed by exposing the membrane to a cytolytic enzyme preparation obtained from the fungus Trichotheeium roseura.
Ферментный препарат (5%) внос т и выдерживают в течение 17-48 ч с толуолом.The enzyme preparation (5%) is introduced and held for 17-48 hours with toluene.
Перед введением ферментного препарата хлореллу стерилизуют (пропаривают ) с 10 ч. воды в течение 1 ч и охлаждают до 40°С.Before the introduction of the enzyme preparation, chlorella is sterilized (steamed) with 10 hours of water for 1 hour and cooled to 40 ° C.
Недостатком этого способа вл ет-, с длительность тепловой обработки (пропаривание до 1 ч ферментативный гидролиз до 17-48 ч), недостаточно полное разрушение клеток хлорел10 лы, что снижает усво емость ее белков при включении в корма дл животных , и низкий выход (11%) белковых веществ из клеток. Кроме того, недостатком способа вл етс добавление The disadvantage of this method is the duration of heat treatment (steaming up to 1 hour by enzymatic hydrolysis up to 17-48 hours), insufficient destruction of chlorella cells, which reduces the digestibility of its proteins when incorporated into animal feed, and low yield %) protein substances from cells. In addition, the disadvantage of this method is the addition of
15 толуола в гидролизуемую смесь и последующее его отделение.15 toluene in the hydrolysable mixture and its subsequent separation.
Целью изобретени вл етс более полное разрушение клеточных оболочек хлореллы и увеличение тем самым The aim of the invention is a more complete destruction of the cell walls of chlorella and thereby increasing
20 выхода белковых веществ.20 output protein substances.
Это достигаетс тем, что тепловую обработку суспензии осуществл ют при 85-95с в течение 3-5 MHg с последующим охлаждением до 45-48 С, а фер25 ментативный гидролиз провод т путем обработки водной суспензии хлореллы культурой гриба Trichoderma lignorum б с в течение 1-3 ч.This is achieved in that the suspension is heat treated at 85-95 s for 3-5 MHg, followed by cooling to 45-48 ° C, and enzymatic hydrolysis is carried out by treating the aqueous suspension of chlorella with a culture of the fungus Trichoderma lignorum b for 1- 3 hours
Claims (2)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762426425D SU1138073A1 (en) | 1976-12-02 | 1976-12-02 | Method of breaking chlorella cell wall |
| SU762426425A SU731935A1 (en) | 1976-12-02 | 1976-12-02 | Method for destructing cell membrane of chlorella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762426425A SU731935A1 (en) | 1976-12-02 | 1976-12-02 | Method for destructing cell membrane of chlorella |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU731935A1 true SU731935A1 (en) | 1980-05-05 |
Family
ID=20685256
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU762426425D SU1138073A1 (en) | 1976-12-02 | 1976-12-02 | Method of breaking chlorella cell wall |
| SU762426425A SU731935A1 (en) | 1976-12-02 | 1976-12-02 | Method for destructing cell membrane of chlorella |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU762426425D SU1138073A1 (en) | 1976-12-02 | 1976-12-02 | Method of breaking chlorella cell wall |
Country Status (1)
| Country | Link |
|---|---|
| SU (2) | SU1138073A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016009145A1 (en) * | 2014-07-18 | 2016-01-21 | Roquette Freres | Method for extracting soluble proteins from microalgal biomass |
| JP2018502592A (en) * | 2015-01-26 | 2018-02-01 | ロケット フレールＲｏｑｕｅｔｔｅ Ｆｒｅｒｅｓ | Method for fractionating components of protein-rich microalgal biomass |
| US10519204B2 (en) | 2014-07-18 | 2019-12-31 | Corbion Biotech, Inc. | Method for extracting soluble proteins from microalgal biomass |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2767055C1 (en) * | 2021-10-14 | 2022-03-16 | Федеральное государственное бюджетное образовательное учреждение высшего образования «Ставропольский государственный аграрный университет | Chlorella shell breaker |
-
1976
- 1976-12-02 SU SU762426425D patent/SU1138073A1/en active
- 1976-12-02 SU SU762426425A patent/SU731935A1/en active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016009145A1 (en) * | 2014-07-18 | 2016-01-21 | Roquette Freres | Method for extracting soluble proteins from microalgal biomass |
| CN106488927A (en) * | 2014-07-18 | 2017-03-08 | 罗盖特公司 | Method for extracting soluble protein from microalgae biomass |
| JP2017521451A (en) * | 2014-07-18 | 2017-08-03 | ロケット フレールＲｏｑｕｅｔｔｅ Ｆｒｅｒｅｓ | Method for extracting soluble proteins from microalgal biomass |
| US10519204B2 (en) | 2014-07-18 | 2019-12-31 | Corbion Biotech, Inc. | Method for extracting soluble proteins from microalgal biomass |
| US10815281B2 (en) | 2014-07-18 | 2020-10-27 | Corbion Biotech, Inc. | Method for extracting soluble proteins from microalgal biomass |
| JP2018502592A (en) * | 2015-01-26 | 2018-02-01 | ロケット フレールＲｏｑｕｅｔｔｅ Ｆｒｅｒｅｓ | Method for fractionating components of protein-rich microalgal biomass |
Also Published As
| Publication number | Publication date |
|---|---|
| SU1138073A1 (en) | 1985-02-07 |
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