SU686732A1 - Arrangement for keeping and showing of placards - Google Patents

Description

I

This invention relates to the field of microbiology and concerns the manufacture of diagnostic products.

A method of obtaining complement from the blood of animals is known by centrifuging it, followed by separating the supernatant, preserving and freeze drying the target product l.

However, the known method is laborious, since the raw material for the production of complement is the blood of guinea pigs, the breeding and maintenance of which is associated with great difficulties and costs, due to their nutrition, maintenance, provision of special facilities, adherence to a certain regime, and staff availability. In addition, blood sampling requires a certain qualification of the performers, since this operation is associated with the introduction of a needle needle into the animal's heart (i.e., cardiac puncture) with the necessary preservation of the latter in a viable state.

The guinea pig is a very small animal, whose weight varies between 25 and 40 g, and its blood content does not exceed 25 t 40 g. The amount of blood serum, which is the basis for the preparation of complement, is very small (7.5 to 18 g from one individual), moreover, this amount of blood is taken repeatedly, in parts, leaving the animal alive.

Due to the instability of the complementary activity of blood sera of different guinea pigs and a small volume of blood samples, the complement is prepared only after the necessary mixing of blood from several animals from at least 50 individuals, all this causes a high cost price of the complement.

The aim of the invention is to simplify the method.

Claims (1)

This is achieved by taking the blood of the whales as the source material and centrifuging it immediately after collection. For example, the surface of the tongue in the proposed place of blood collection is treated with 96% ethanol, tissue of the tongue is excised into deep blood vessels. At the same time, blood flows freely under natural pressure and is collected in a 2O liter container. Then the blood is poured into 15 ml centrifuge tubes and centrifuged for 30 minutes at 3000 rpm. The supernatant (plasma) is sucked off with a pipette and poured into a 6-liter dark glass bottle. 5% w / w UMe 30 (chemically pure) and 4% acid are injected into the same bottle, mixed thoroughly, the bottle is placed in a refrigerating chamber and kept in it for 20 hours at - O - + 4 C. The resulting complement is bottled 1 ml in sterile BCG ampoules from 6 ml neutral glass, each ampoule closed with a loose wadded stopper. After that, freeze drying, sealing of ampoules and other operations are carried out in a known manner. The proposed method is simple to implement, does not require expensive or unique equipment. From one whale with an average weight of 35 tons, 1.5-1.7 tons of blood is obtained, which is equivalent in terms of weight to 80 thousand h of guinea pigs. Blood sampling does not require a special qualification from the executor, and the exclusion of mixing and settling operations makes it possible to shorten the time of complement production. The invention The method of obtaining Complement from the blood of animals by centrifuging it followed. separation of the supernatant, preservation and freeze drying of the target product, characterized in that, in order to simplify the process, whale blood is used as a starting material and centrifuged directly after the collection. Sources of information taken into account in the examination 1. MRTU - 42 № 128-66.