SU657063A1 - 1c sp. pseudomonas strain for purifying industrial waste water from alkylsulfonates - Google Patents

Description

one

The invention relates to the microbiological industry and can be used in the biochemical treatment of wastewater from detergents based on alkyl sulfates, for example, textile and camouflage plants.

A known strain of Citrobacter freundii 2T, destroying the dodecyl sulfate sodium at a concentration of 3 g / l I.

However, the known strain does not decompose technical alkyl sulfates, which are a mixture of various homologues of alkyl sulfates with an admixture of unreacted raw material and products of side reactions.

The aim of the invention is to increase the activity of destruction and the expansion of the destructive capabilities of the strain, the alkyl sulfate destructor.

The resulting strain Psetsdomopas sp. 1C has the following morphological and physiological characteristics: direct movable rods, cells are located singly or in short chains, gram-negative spores and capsules do not form;

On the m-coopton agar, the colonies are flat, rounded with sinuous or even edges, the structure is granular, translucent in transmitted light, gray with metallic oTteHKOM, diameter 5-8 mm.

On the m-peptone broth, the culture causes a very weak clouding of the medium, forms a leathery film, releases a fluorescent pigment.

The oxidase test according to Kovach is positive, the culture forms levites from sucrose, hydrolyzes Tween-80, dilutes gelatin, has lecithinase, does not hydrolyze starch, does not restore nitrates; does not form indole and hydrogen sulfide, milk peptonizes, assimilates mineral forms of nitrogen; under anaerobic conditions, glucose does not decompose.

Glucose, arabinose, xylose, mannitol, salicin, glycerin are used as the sole carbon source on the Omel Nskogo mineral medium; does not use lactose, maltose, sucrose, dulcnt, sorbitol, inositol.

. Strain Pseudomonas sp. 1C is separated from the wastewater treatment plant in Kiev in Bortnichy.

Claims (1)

 The proposed strain destroys 7 g / l of sodium dodecyl sulfate in a mineral medium, using it as the only carbon atom. In the same mineral medium, this strain destroys I g / engineering preparations of alkyl sulfates. Strain Pseudomonas sp, 1C purifies waste water containing 1 g / l of technical preparations of alkyl sulfates. Example. Strain Pseudomonas sp. 1C destroys in the mineral medium of the following composition, in g / l: KHjPO, - 3.0; K.NRO. - 7.0; NH, NO - 1.0; KCl - 0.5; MgCb - 0.1; pH - 9.0-7 g / l dodecylsulfate for 48 h, at a growing temperature of 28-30 ° C. On the same mineral medium under the same conditions of cultivation, this culture destroys 1 g / l of technical alkyl sulfates for 24 hours.;. The activity of the Pseudomonas sp-1C strain increased compared with the Citrobactertreun dii 2t strain with CO and its possibilities expanded: so, unlike Citrobacter freundii 2t, Pseutfomonas sp 1C destroys higher concentrations of dodecyl sulfate (not 3 g / L, but 7 g / L), and also destroys technical alkyl sulfates. Using strain Pseudomonas sp. 1C, it is possible to locally purify industrial wastewater with a high content of alkyl sulfates. An experimental industrial verification of the strain is scheduled for 1979-1980 at the Pervomaisky chemical plant. Claims of the invention: Pseudomonas sp. LC strain for the purification of industrial wastewater from alkyl sulfates. Stored in the collection of the KhKHV AN USSR DoyM / ZO .. Morphological signs. Movable straight or slightly curved rods. Cells are located singly or in short chains. Gram-negative, spore and capsules do not form. Cultural features. M co-peptone agar: flat colonies, rounded, with sinuous or even edges, a granular structure, translucent in transmitted light, gray with a metallic tint. M-co-peptone broth: weak turbidity of the medium, leathery film, culture emits a fluorescent pigment. Physiological and biochemical signs. The oxidase test is positive, it does not restore nitrates, forms levau from sucrose, hydrolyzes tween-80, starch-nehrolysis, has lecithinase, gelatinase. Indole and hydrogen sulfide does not form. Peptonized milk. Absorbs mineral forms of nitrogen. Relation to carbon sources: manhrft, salicin, glycerin. Use glucose, .arabinozy, xylose. Does not use lactose, maltose, sucrose, dulcite, sorbitol, inositol. Aerobe, the optimum cultivation temperature is 28-30 ° C. Strain Pseudomonas sp. 1C destroys up to 7 g / l dodecyl sulfate and up to 1 g / l technical preparations of alkyl sulfates in a mineral medium. Sources of information taken into account during the examination 1. USSR Author's Certificate No. 560906, cl. From 12 to 1/00, 1975.