SU943281A1 - Strain nocardia sp.km-2 to be used for purifying effluents from pyridine - Google Patents

Description

The invention relates to the microbiological industry and relates to the production of a new type of Stms1MmA, which can be used in the biological treatment of advanced wastewater from a number of microbiological, coke and medical industries from pyridine.

A known strain of Nocardia sp.L-1, using pyridine as a source of carbon and nitrogen Cl.

However, this is characterized by growth at lower concentrations of pyridine (up to 0.1 vol.%), As well as the lack of data on the use of other toxic compounds by the strain.

A known strain of Corynebacterium sp., Utilizing pyridine C21.

However, this strain is also characterized by the growth of the strain at lower concentrations of pyridine (up to 0.15% by volume), the need of the strain for an additional source of nitrogen, and the lack of data on the use of other toxic compounds by the strain.

Shtg1mm Nocardia sp.KM-2 can use pyridine as the sole source of carbon and nitrogen in concentrations up to 0.2% by volume. In addition, the strain Nocardia sp. KM-2 utilizes the following compounds found along with pyridine in industrial wastewater: phenol, m-cresol, benzoic acid, 3-hydroxybenzoic acid, p-aminobenzoic acid, 3-phenylpropionic

10 acid (in a concentration of 0.1-0.4%). Strain Nocardia sp. CM-2 was isolated from the soil from the territory of the coke-chemical plant.

The strain has the following characteristics.

Morphological and cultural characteristics.

Gram cells are positive, immobile, unchallenged, acid-beneficial, morphologically altered during growth: mycelium consisting of long filaments (1-1 µm) is fragmented to short rods (1-1.3 µm) 24-30 h of culture growth on solid agar medium.

On m and-peptone agar colonies are rounded, 1-3 mm, dull, pink, smooth, convex, homogeneous, pasty consistency, macaw

straight. With aging, the color of the colonies changes to pink-red, the structure becomes fine-grained.

On mо-peptone broth - ro | 3а, dry, sploina film, a small residue.

Physiological and biochemical signs.

Strict aerobic. The optimum growth temperature is 28-30 s. Maximum growth temperature. The optimum pH is 6.5-7.2. Growth is not inhibited by B, 5% Nace. Catalase positive and oxidative. No additional growth factors are needed. Forms acids during growth on glucose, fructose, galactose, arabinose, xylose, sucrose, maltose, mannitol, sorbitol, dulcite and glycerin. Does not assimilate lactose, raffinose and rhamnose.

Forms ammonia and hydrogen sulfide. Does not form acetylmethylcarbinol and indole. Nitrates restores to nitrites. Gelatin is not diluted, starch and casein do not hydrolyze. Litmus milk does not roll.

The nucleotide composition of DNA. (Mol.% G 71.4.

Example. Strain Nocardia sp. KM-2 is grown on synthetic sde of the following composition, g / l:

NaaHP044,26

. 2.65

MgS04.x, 2

MnSO4x7H 2 O0.002

Re8rfh7N, O0.01

CaСе0,02

NaijMoO 0.001

Distilled water to 1 liter, g, as a source of carbon and nitrogen, use pyridine, introduced at a concentration of 0.1-0.2% by volume. pH of media7, 0-7.2. The cultivation is carried out at 28-30 ° C on a rocking chair 200 rpm

2-3 days depending on the initial concentration of pyridine. The inoculum is grown under the same conditions and introduced into the medium in a concentration of 5-10 vol.

Completeness of pyridine consumption was determined by chromatography of the chloroform extract of the culture liquid after biomass was deposited on a chromatography – mass spectrometer and spectrophotometrically on an SF-203 spectrophotometer.

The proposed strain is more effective than the known ones, as it is capable of utilizing pyridine as the sole source of carbon and nitrogen in a concentration of up to 0.2% (against 0.1-0.15% of known strains) and at the same time a number of toxic compounds found in the wastewater of coke production, enterprises of microbiological and medical industry.

Claims (1)

1.G.K.Watson, R.B.Cain. MicrobiaB metaboeisra of the Pyridine Ring. Jhe BiochemicaE journal. 1975, voe. 146, No. 1, 151-172. 2 .O.P.Shuk € a, S.H.KauE. A.Constitutive Pyridine Degrading Systemin Corynebacteriiim sp. The. Indicin Jourmae of Biocheroistryand Biophysic 1974, voe 11, 201-207.