SU653251A1 - Dimethylcetylbenzylammonium chloride showing virucidal activity - Google Patents

Description

Len5sh. The yield of dimetighetilbenzylmonium chloride is 92%.

Found%: N3.00 NCe

Calculated,%: Y 3.50.

Experimental studies on the antiviral activity of dimethyl acetyl benzyl ammonium chloride were carried out on Venezuelan viruses: horse endephalomne (attenuated strain VAL-.23 O), herpes simplex (Schgamm L 2), fixed rabies virus (x), and bacterial viruses, and the haem off-onset viruses, a combination of rabies viruses (Schgamm L 2), fixed rabies virus (x), and bacterial viruses, and a combination of them, and other bodybuilding viruses have been tested. Vitro AI, a common experiment: an equal volume of various concentrations of the compound is added to the virus suspension. The mixture is kept at different times and then titrated. Virus samples processed under the same conditions are used as controls.

The viruidal activity of the compound against VAL and herpes viruses was assessed by Spisionly Icfeccinia 1st activity of viruses in Tissue fibroblasts tissue culture; against the virus

fi heh - on the survival of mice; in relation to bacterial viruses T and f on the cross-linking of the infectious activity of phages reproduced on the corresponding bacteria of the host.

Positive control was introduced into each experiment. Ethyl alcohol n chloramine was used as a positive control for VAL virus, chloramine for fi xe virus, and chloramine for viruses bacterial viruses, chloramine, lyzol, hydrogen peroxide, for chloramine virus and lysol. As a result of the study, a pronounced virucidal effect of dimethyl acetal benzyl ammonium chloride on the studied viruses was established. Table 1 presents the results of the study of dimethyl acetylbenzylammonium chloride against the VAL virus.

Comparative assessment of virucidal activity

dimethyl acetylbenzylammonium chloride and known viropicidal agents against VAL

Note: virus titer is 6.02 1 o-BFU / ml.

Table

At a concentration of 500 µg / ml, the compound had a complete inactivating effect on the VAL virus already under five minutes of exposure of the virus to the compound. Chloramine and lysol in the same concentration had almost no effect. A pronounced activity of dimethyl ethyl benzyl ammonium chloride with respect to the herpes simplex virus was noted. Dose of 50 Og / ml caused complete inactivity of the viricidal activity of dimethyl-acetyl-benzylammonium chloride in relation to the herpes simplex virus

Note: virus titer 3,5 Ig TID Q i

With respect to the fixed rabies virus, the virus inhibiting effect is determined by the survival of the animals, taking into account the number of neutralized absolutely lethal doses of the virus. In table 3 presents the activity of the drug against the virus fi he. Compound

virus. A good Vitrulyil PChT effect was achieved in doses of 1 CO and 2 O µg / ml (a decrease in virus titer by 3.27 and 1.5 1 g, respectively). Xnoj amine, only at a concentration of 500 µg / ml, caused a decrease in virus titer of not 2,0 Igf, while concentrations of 100, 20, and 5 turned out to be inadequate. Lysol activity was also weak in relation to the herpes virus.

Table 2

exposure time 18 hours.

45 At concentrations of 4OO, 2OO, 1OO µg / ml, when exposed to the virus for 15 minutes, 1000-100 b salt-lethal doses of the virus were neutralized. Activity dimethylcytilbenzylammonium: slorida

SO was also maintained at five intact exposures with the virus.

Varupicidal activity of dimeg pceipbenzplammon jlorip in relation to a fixed rabies virus

A pronounced virucidal effect of dimethyl acetylbenzylammonium chloride was obtained

Compare the known virucidal effect of dimethyl petyl benzylpammonium chloride on known virus preparations against bacterial viruses T and I-.

. X well

./1nmetiltsvtnlbvnavsyammonvy

chloride

Hydrogen peroxide

Rivanol

in experiments with bacterial viruses T „and t

 AO

Table 4

9

Notes: 1. A dose of a compound of 100 µg / ml 2, an Exposure Concentration of T. viruses, and a compound for 18 hours led to their complete inactivation. A significant virucidal effect was also noted at exposure at 1 and 4 o'clock (decrease in T virus titer by 1.5 and 4.3 Ig and Ig virus by 3, O, and 6.0 1 sg, respectively). Marked superiority :; the proposed viriliiida compared with the known disinfectants such as p vanol, hydrogen peroxide, lysol. The last two compounds almost did not have any bacterial viruses T c $ „on aiiTHBiiocTb. Only chloramine with „exposure at 1 and 4 o'clock is more effective than the proposed virucide. The data presented indicate that dimethyl-acetyl-benzylammonium chloride is a viable BH anti-toxic agent for the control of animal viruses (herpes simplex, Venezuelan equine encephalomyelitis, and fixed rabies virus) and viruses

65325110

Claims (3)

f. Continuation of the table 4 Hydrogen oxide 0,0001I). bacteria (coliphages T and f) The indicated compound is known for its effectiveness of known virucidal agents. Claim Dimethylnylbenzylammonium chloride Formula cH-j-MtcHg-CfeHsJce, 16 for exhibiting virucidal activity. Sources of information taken into account in the examination 1. US patent number 3439100, cl. 424-325, 1974. 2. The patent of France No. 2О81402, кл. 27/00, 1974. 3. The copyright certificate; of the USSR No. 381684, cl. From 12 to 7 / OO, 1974.