SU649407A1 - Device for introducing microelectrode into movable cell - Google Patents
Device for introducing microelectrode into movable cellInfo
- Publication number
- SU649407A1 SU649407A1 SU762371667A SU2371667A SU649407A1 SU 649407 A1 SU649407 A1 SU 649407A1 SU 762371667 A SU762371667 A SU 762371667A SU 2371667 A SU2371667 A SU 2371667A SU 649407 A1 SU649407 A1 SU 649407A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- microelectrode
- introducing
- movable cell
- chamber
- tube
- Prior art date
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
тельным электродом. Нижн часть камеры 7 присоединена к трубке 11, в которой по центру неподвижно установлен микроэлектрод 12, контакт которого выведен через держатель 13 и герметизирующую трубку 11 наружу. Трубка 11 соединена трубопроводом 14 с камерой 2. Микроэлектрод 12 соединен с усиливающей и регистрирующей аппаратурой.body electrode. The lower part of the chamber 7 is connected to the tube 11, in which a microelectrode 12 is fixedly mounted in the center, the contact of which is brought out through the holder 13 and the sealing tube 11 to the outside. The tube 11 is connected by pipe 14 to the chamber 2. The microelectrode 12 is connected to the amplifying and recording equipment.
Такнм образом, камеры 2 и 7 размещены одна под другой. Верхн вл етс резервной , нижн - камерой давлени , так как она сообщена с пневмосистемой, а держатель с микроэлектродом установлен в трубке 11, сообщенной с той и другой камерами .Thus, chambers 2 and 7 are placed one below the other. The upper one is a backup, the lower one is a pressure chamber, as it communicates with the pneumatic system, and the holder with the microelectrode is installed in the tube 11 communicated with both cameras.
Устройство работает следующим образом .The device works as follows.
При определении, например, биопотенциалов клеточных мембраи подвижных клеток крови берут необходимое количество крови и в пробу добавл ют 0,1% нитрата натри дл предупреждени свертывани . Ползченнзю исследуемую среду помещают в резервную камеру 2, из которой через открытый перепускной клапан 6 исследуема среда перемещаетс в камеру 7 давлени , до полного ее заполнени . Перепускной клапан 6 закрывают и через патрубок 8 нагнетают стерильный воздух. Под действием последнего исследуема среда через трубку // поступает по трубопроводу 14 в камеру 2, омыва при этом микроэлектрод 12, на который нанизываютс клетки, передава свой биопотенциал через микроэлектрод 12 на усиливающую и регистрирующую аппаратуру. Отрицательным электродом в камере давлени вл етс пробка-винт 10. По окончании полного перемещени исследуемой среды цикл повтор ют нужное количество раз.When determining, for example, the biopotentials of the cell membranes and motile blood cells, take the required amount of blood and add 0.1% sodium nitrate to the sample to prevent clotting. The test medium is crawled into the backup chamber 2, from which, through the open bypass valve 6, the test medium moves into the pressure chamber 7 until it is completely filled. The bypass valve 6 is closed and sterile air is injected through the pipe 8. Under the action of the latter, the test medium through the tube // enters through the pipeline 14 into the chamber 2, washing the microelectrode 12 on which the cells are strung, transferring its biopotential through the microelectrode 12 to the amplifying and recording equipment. The negative electrode in the pressure chamber is the plug-screw 10. At the end of the complete movement of the test medium, the cycle is repeated the necessary number of times.
Устройство позвол ет без утомительных манипул ций вводить микроэлектрод в любую клетку, особенно в подвижную. С его помощью можно снимать биопотенциал поко у эритроцитов и других клеток живого организма, а также изучать изменение биопотенциала поко различных клеток по степени их травмировани микроэлектродом .The device allows, without tedious manipulations, to introduce a microelectrode into any cell, especially a mobile one. It can be used to remove the resting biopotential of erythrocytes and other cells of a living organism, as well as to study the change in the biopotential of different cells according to the degree of trauma by a microelectrode.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762371667A SU649407A1 (en) | 1976-06-14 | 1976-06-14 | Device for introducing microelectrode into movable cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU762371667A SU649407A1 (en) | 1976-06-14 | 1976-06-14 | Device for introducing microelectrode into movable cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU649407A1 true SU649407A1 (en) | 1979-02-28 |
Family
ID=20665327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU762371667A SU649407A1 (en) | 1976-06-14 | 1976-06-14 | Device for introducing microelectrode into movable cell |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU649407A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5361760A (en) * | 1989-11-07 | 1994-11-08 | University Of Utah Research Foundation | Impact inserter mechanism for implantation of a biomedical device |
-
1976
- 1976-06-14 SU SU762371667A patent/SU649407A1/en active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5361760A (en) * | 1989-11-07 | 1994-11-08 | University Of Utah Research Foundation | Impact inserter mechanism for implantation of a biomedical device |
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