SU647339A1 - Strain pseudomonas aeruginosa 640x - Google Patents

Description

(54) SHTAMM Psaudomonas aerug-ihosa 640x

The invention relates to the field of microbiological research and represents a new strain that can be used for the destruction of DDT, /.

Microbiological methods, decomposing DCTs, are now of great importance. Major. Efforts are aimed at obtaining cultures of microorganisms capable of decomposing DTTs to non-toxic products. Significant success, however, has not been achieved until recently. It has been established that some microorganisms are able to transform DTD in DDDD, DDE and DBP l.

The splitting of DDT has been achieved. also using the culture of the fungus PeniciCiomsp. ATCC 20.369 2j |.

However, in the description of the patent, only data on the destruction of DTs in the Environment without the characteristic of conversion products and the intensity of the process are given. . -.

The aim of the invention is to isolate the strain Pseudomonois aeruginosoi 640x, which has the ability to degrade DDT, including complete dechlorination and the disclosure of one of the IZ rings of the pesticide molecule.

. The proposed Pseudomonas aeru-fnosa 640x strain is characterized by the following features. The strain degrades the DDT molecule of phenylacetic acid during the growth process on hexadecane.

 Morphological and cultural characteristics. The strain was isolated from the soil of the Orchard of the Crimea region, for .23 years. process uvaem

0 DTT. Cells are straight, rod-shaped, sizes 0.7 - 1.0 (single cells 1.5) X 0.3 g, 0.5 mK. J. motile, monotrichs have fimbriae, gram-negative, risperadone.

five

M so-peptone agar. The colonies are light gray, flat, deep, shiny, with even edges. Yellow-green, fluorescent pigment is extracted into agar.

0

M so-peptone broth. The slurry, film and fluorescent pigment are formed. . Mineral E-8 with glucose. The colonies are white, smooth, shiny,

5 flat, with a smooth edge, no pigments are released into the medium.

Wednesday Capek. Colonies are small / white, smooth, shiny, flat, with a smooth edge, pigments on Wednesday

Claims (2)

0 is not selected. & Glucose-potato agar. Colonies of light-colored, smooth, shiny, pOBHfcjfi edge, pigments are not isolated on Wednesday. King A. Medium. Colonies are pale-naked, smooth, shiny, segregated in medium-blue pigment-pyocyanin, King's Medium B. Colonies are bright, yellow-green, smooth, shiny, secrete S cpejjy fluorescent - Fioligricus and biochemical claims. Aerobe gives a positive oxidative reaction and does not accumulate a poly jb-hydroxy-buty acid in cells. Grows at 41s / ne p1a :: tet at 1-10 ° C, optimally teyPe | atura dl: height 29-30c. . . : Attitude towards sugars. Assimilate X fructose, xylose, sucrose, trehNaOeu, I) - glucose. Does not absorb riboeu, arabinose, ramibzu ;, r ffinoeu, lactose, maltose, galactose: bbarazuyuet acids on xylrze and fruit ze. -:. .. -:. -;.,.; , ---; Attitude to organic acids Absorb to acetate, propionate, citrate pyruvate, malate, succinate, benzoate ,, h -oxybenzoate, prdtokatohat. Does not displace tartrate, p-toluate. . ; Attitude to carbohydrate. Not boil naphthalene, p-xylene, pcresol, phenol, biphenyl. It grows on n-alkanes from C to C, RatioK alcohols. Absorbs ethanol and glycerin, does not absorb the Sbr bit. ;:,; , -,, ;. . Amino acid resistance. Absorbs Yalin, and Agini rginina, relates to the sources of nitrogen. Does not assimilate nitrate nitrogen, assimilates ammonium nitrogen. : Reduced nitrates are absent. Nitrate breathing is positive, Thins gelatine, casein hydro LIZUET. :,; . . . In distinguishing FROM PREVIOUS Ms. Yikrob1 ha1njans pseuda Pseudomonas ae-aug-iiT06ei b40x performs a complete dechlorination of the molecule, pro, ductrm is veasteto, it is easy to use; DTT, process. And | {em at higher initial con centrations; DDT and with significantly greater intensity. . A feature of the process is that it is carried out in the process of culture growth on n; hexadakane. The proposed strain Pseudotnonas aerug4nosa b40x can be used to degrade DDT, which can be present as a contaminant in water, discharged sewage, in different soils contaminated with DDR, and the process of purification from DDT can be ignored when natural sources of oil products are contaminated. since ultrasultates the degradation of CT in the process of growth on n-alkanes. Claims; The strain Pseudomonasaerugrinoba b40x is stored in the crop collection of the Microbiological Transformation Department of Organic Conjunctions of the IBPM Afl USSR. Designed to decompose DTT ..; Morphological and cultural features. Strain. Centrified from the soil of the orchards of the Crimean region, for 23 years processed DTT. .-:. , . Small stick, O, 7-1,0x0,3-0,5 microns, individual cells reach 1.5 glkm, cells are motile, monotrichi, gram-negative, spontaneous. M sdeptynny agar. The colonies are light gray, flat, smooth, shiny, with smooth edges. A yellow-green fluorescent pigment is extracted into the agar. . . -. M, co-peptone broth. Formed turbidity, film fluorescent pigment.;:;.. f- Mineral medium E-8 with glucose, Colonies are white, smooth, shiny, flat, with a smooth edge, there are no pigments in the medium. are highlighted. . Wednesday; ; Capek. The colonies are small, white, smooth, shiny, flat, with a smooth edge; no pigments are isolated into the medium. ;. . . . ; : Glucose-potato agar. Colonies. light-brown, smooth, shiny, smooth edge, no medium pigments are isolated ... ... ......... Wednesday Kinea A. The colonies are pale blue, smooth, shiny, placed on Wednesday blue pigment-pyotsyantsn. -;,, . ; King B. Wednesday. Colonies are light, yellow, green, smooth, shiny, and fluorescent pigment is released into the medium. ; . . . ., Physiological and biochemical signs. Aerobe gives a positive oxidase reaction; it accumulates in cells poly - | - hydroxybutyric acid. It grows at, does not grow at, the optimum temperature for growth is 20-30 seconds. Attributes to sugars. It absorbs D-fructose, xylose, sucrose, trehalose, b-glucose. Does not digest ribose, raffinose, arabinose, rhamnose, lactose, maltose, galactose. Forms acids on xylose and fructose. Relation to organic acids. Absorbs acetate, propionate, citrate, pyruvate, malate, succinate, benzoate, p-oxybenzoate, prostokatohat, does not use tartrate, p-toluate. Relation to hydrocarbons. Does not absorb naphthalene, ti-xylol, p-5 cresol, phenol, biphenyl. Grows on n-alkanes from Cjg. Attitude to alcohols. Absorbs ethanol and glycerin, does not absorb sorbitol. Relation to amino acids. Assimilates arginine, valine, alanine. Relation to nitrogen sources. N assimilates nitrate nitrogen, assimilating ammonium nitrogen. Reduction of nitrates is absent. 9 Nitrate breathing positive Gelatine thins, casein gIarrlizuet. Sources. Information taken into account in the expertise of ipatitK.et “e. pegrradalion of end-, g in aeobrtn BDT tv Soie microopgraniam ApptWicrofcioe., t97O, 9; c.879-881 2. US patent 3979283, cl. 210-11, 1976.