SU644826A1 - Apparatus for isolating and concentrating microorganisms - Google Patents

Description

one

The invention relates to flotation devices for the isolation and concentration of microorganisms, such as yeast grown in carbohydrate and hydrocarbon media, and can be used in microbiological, alcohol, bakery and other industries.

A known apparatus for isolating and concentrating microorganisms from the culture fluid, comprising a container with nozzles for supplying the culture fluid, an aerator located in the lower part of the container, a device for executing foam in the form of a vertical auger installed in the container, and an antifoam 1.

Such a device is closest to the apparatus, made according to the invention to the technical essence and the achieved result.

A disadvantage of the known apparatus is the formation of stagnant zones on the periphery of the vertical auger due to the fact that the speed of movement of the foam in the interturn space of the dryer is not the same: the highest in the central part of the device for drying the foam, the smallest at the periphery.

The difference in the velocity of the foam causes the foam to move only along the central part of the device to dry the foam. In this case, the volume of the drying part of the apparatus and the surface of the drying are not fully utilized.

This leads to the fact that the productivity of a unit volume of the apparatus (specific) decreases and the degree of biomass concentration in the foam decreases.

The purpose of the invention is to increase the concentration of microorganisms in the thickened suspension and increase the specific productivity.

This is achieved by installing a shell in a container concentric with it, with the auger branches attached to the outer surface of the shell, and inside it in a checkerboard trays for additional drying of foam, with the antifoam placed in the upper part of the shell above the trays.

FIG. 1 shows schematically an apparatus for isolating and concentrating microorganisms from a culture fluid made in accordance with the invention; in fig. 2, 3 — trays for additional drying of the foam; in fig. 4 - section A-A of FIG. 2

The described apparatus contains a container 1 with nozzles 2 for supplying a culture fluid.

In the lower part of the tank 1, tangentially around the circumference, ejection aerators 3 and generators 4 and 5 are located to output the circulating clarified liquid.

In the tank 1, concentric with it, a shell 6 and a device for drying the foam are installed.

The latter is made in the form of a vertical screw 7, the turns of which are fixed on the outer surface of the shell 6.

Trays 8 are installed inside the shell 6 to further dry the foam. Trays 8 have the shape of a gutter with a slope from the middle to the edges of the m.

In order to ensure the cross movement of the foam, the trays are arranged in a staggered order so that the superior tray is located above the gap between the trays of the lower row.

An antifoam 9 is placed above the trays 8 in the upper part of the shell. There is an electric motor 10 for communicating the rotational movement to the last one. For supplying circulating clarified liquid, the device is equipped with a pump I.

In addition, the apparatus is equipped with fittings 12 and 13, serving respectively for the withdrawal of exhaust air and concentrated biomass.

The device works as follows.

The original culture fluid through the nozzles 2 enters the lower part of the tank 1.

In the lower part, flotation of microorganisms, for example, yeast, with fine air bubbles obtained using ejection aerators 3 occurs. Ejection aerators suck air from the atmosphere due to the energy circulating through them under pressure from a clarified culture liquid. The circulating clarified culture liquid is taken from the lower part of the apparatus through fitting 4 and pumped AND is fed to the aerators 3. The sucked air in the ejection aerator is mixed with the clarified liquid, crushed into fine bubbles and the gas-liquid mixture flows into the lower part of the tank 1.

Flowing downward through the lower part of apparatus 1, the initial culture fluid is clarified from the cells of the microorganisms by adhesion to the floating air bubbles.

The foam formed in the upper part of the tank 1 is divided into two streams, while the outside stream of the foam rises upwards along the vertical auger 7 and dehydrates, and the liquid flows out of the interbubble space of the foam under the influence of gravity.

The presence of the shell 6 ensures uniform foam movement through the interturn space of the screw by equalizing the speeds of the foam at the outer and inner portions of its turns, which prevents the formation of stagnant zones at the outer portion of the screw.

The internal foam flow receives cross-movement: vertical - in the gaps between the trays and horizontal - in the space between the rows of trays. With this movement of the foam, the reflux liquid flows intensively out of it, enters the trays 8 and, due to their inclination from the middle to the edges, flows down to the walls of the shell and, without contact with the rising foam, returns to the bottom

part of the apparatus.

The concentrated foam enters the defoamer 8, is quenched, and is discharged from the apparatus through shtutser 13. The clarified culture liquid is withdrawn from the apparatus through nipple 5.

The use of the invention will increase the concentration of microorganisms in the condensed phase, which will reduce the capital and operating costs of

its additional processing and increase the specific productivity of the device.

In addition, the withdrawal of the condensed phase directly from the body of the defoaming agent improves the quality of the finished product, as long as the condensed phase remains in the apparatus.

Claims (1)

1. For the number 1975631 / 28-13; cl. With 12С 11/24, according to which a decision was made to issue an author's certificate.