SU643480A1

SU643480A1 - 329b rhizobium trifolii strain of red clover nodule bacteria

Info

Publication number: SU643480A1
Application number: SU772534611A
Authority: SU
Inventors: Эдмундас-Антанас Брониович Лапинскас
Priority date: 1977-10-14
Filing date: 1977-10-14
Publication date: 1979-01-25

Description

ОтноаТение к углеводам и спиртам. Усваивает глюкозу, галактозу, cajfapoзу , мальтозу, лактозу,рафиноэу, маН нит с небольшим подкислением , Лучше всего усваивает сахарозу к нит, хуже - лактозу.Relation to carbohydrates and alcohols. It absorbs glucose, galactose, cajfapoz, maltose, lactose, rafinoe, mannit with a slight acidification, it is best absorbed by sucrose to nit, worse - lactose.

Отношение к источникам азота, Использует азот (МНд)ЗО, ,Relation to nitrogen sources, Uses nitrogen (MND) DA,

KNo И карбамида.KNo and carbamide.

Молоко поптонизирует с образованием прозрачного кольца на поверхности молока в пробирке. Среду подщелачивает ,,Milk poptonizes with the formation of a transparent ring on the surface of the milk in a test tube. Alkalizes Wednesday ,,

Крахмал и клетчатку не гидролизуеStarch and fiber are not hydrolyzed

Нитраты йоеетанавливает,Nitrates can be determined

Желатину не разжижает,Gelatin does not dilute

Штамм клубеньковых бактерий Rti-izotnum tfi loiH 32S6 выделен из клуОеиьков красного клевера, возделываемого а колхозе им,В.И Ленина Шак Пекого района Литовской ССР на дерноноподзолистык лагкосуглиниатых хорошо окультуренных почвах. Из 120 выделенных штаммов из разных почв республики при изучении эффективности был отобран среди наилучших 3296.The strain of nodule bacteria Rti-izotnum tfi loiH 32S6 was isolated from red clover plants cultivated on the collective farm named after V.I. Lenin Shak in the Peko district of the Lithuanian SSR on a lodgosudaluminous, well-cultivated soil. Of the 120 strains isolated from different soils of the republic, the efficiency was selected among the best 3,296 when studying effectiveness.

Результат продолжительного изучени 1969 197бгГе) эффективности инокул ции раннеспелого (двухкосного)The result of a lengthy study of 1969 (197 bgGe) of the efficiency of inoculation of early ripening (two-barred)

красного клевера сорта Лепена известными и предложенным штаммами привод тс в таблице.Red clover varieties Lepena known and proposed strains are listed in the table.

Новый штамм 3296 в услови х вегетационных опытов при инокул ции по .высил урох(ай абсолютно сухой массы клевера на 47% по сравнению с инокулрованными более эффективным произаодственным штаммом 31 la. Аналогичные результаты получены и в полевых опытах, где предлагаемый штамм способствовал прибавке абсолютно сухого ееиа клевера в первом году пользовани траэ на 8,1 ц/га, в то врем как штамм ЗИа - на 4,7 ц/га.A new strain 3296 under the conditions of vegetative experiments when inoculated according to height increased (47% of the absolutely dry mass of clover by 47% compared to the inoculated with a more effective 31 la strain. Similar results were obtained in field experiments where the proposed strain promoted the addition of absolutely dry in the first year of trae, the ikels of clover were 8.1 centner / ha, while strain ZIa 4.7 centner / ha.

Инокул ци не имела существенного вли ни ка процентное содержание сырого протеина в урожае. Однако при положительном действии нового штамма на урожай клевера получена существенна прибавка в сборе сырого протеина по сравнению с контролем 1 ,16 ц/га,Inoculum did not have a significant effect on the percentage of crude protein in the crop. However, with the positive effect of the new strain on the yield of clover, a significant increase in the collection of crude protein was obtained compared with the control of 1, 16 c / ha,

Claims

In relation to virulence, strain 3296 occupied an intermediate position between the more virulent strain 348a and the less virulent ZIA, which is also confirmed by the data on the formation of nodule on the roots of plants. 564 Claim Invention The strain of red clover nodule bacteria Rliizobium tPifoCii .3296 is used to make nitragin for red clover. Stored in collections of the All-Union Scientific Research Institute of Agricultural Microbiology. Morphological and cultural signs. At a young age, the bacteria are rod-shaped, the size of the cells on bean agar is 0.8x2.3 microns. It is well colored in red with a solution of 3% erythrosine and 5% phenol. Physiological and biochemical properties. Organic environments. On the bean medium (pH 7.2), the colonies are smooth (form S), round with smooth edges. The diameter of the colonies of the daily culture is at a growing temperature of 25 ° C 15 mm. On the medium MPA growth is completely absent. Norris Synthetic Medium with other extracts. The colonies are smooth (form S) with smooth edges, the diameter of a daily culture is 15 mm at pH 6.5. Relation to the carbon source, Absorbs glucose, sucrose, galacto ZU, maltose, lactose, raffinose, mannitol with a slight acidification of the medium. It best absorbs sucrose and mannitol, worse - lactose. Attitude to sources of nitrogen. Uses nitrogen (NH) SO,, KNOj and carbamide. Milk peptonized with the formation of a transparent ring on the surface. Alkaline Wednesday. Gelatin does not dilute. Starch and fiber do not hydrolyze. Nitrates restores. Temperature optimum is 21 ° C. The optimum response of the medium is pH 6.5. On synthetic medium, growth is observed at pH 4.0 to 10.5. Sources of information taken into account during the examination: 1. Conscious of Yu.M. Plant microflora and harvest. L., Kolos, 1969, p. 92-98.

2. Lapinskas E, B. On the effectiveness of cross inoculation of clover by various strains of nodule bacteria. Microbiologists, v.44, issue 4 1975, p. 695-699.