SU638599A1 - Method of obtaining nucleic acid fractions - Google Patents

Method of obtaining nucleic acid fractions

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Publication number
SU638599A1
SU638599A1 SU772506145A SU2506145A SU638599A1 SU 638599 A1 SU638599 A1 SU 638599A1 SU 772506145 A SU772506145 A SU 772506145A SU 2506145 A SU2506145 A SU 2506145A SU 638599 A1 SU638599 A1 SU 638599A1
Authority
SU
USSR - Soviet Union
Prior art keywords
dna
fractions
nace
concentration
nucleic acid
Prior art date
Application number
SU772506145A
Other languages
Russian (ru)
Inventor
Леонид Сергеевич Попов
Original Assignee
Московский Ордена Ленина И Ордена Трудового Красного Знамени Государственный Университет Им. М.В.Ломоносова
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Application filed by Московский Ордена Ленина И Ордена Трудового Красного Знамени Государственный Университет Им. М.В.Ломоносова filed Critical Московский Ордена Ленина И Ордена Трудового Красного Знамени Государственный Университет Им. М.В.Ломоносова
Priority to SU772506145A priority Critical patent/SU638599A1/en
Application granted granted Critical
Publication of SU638599A1 publication Critical patent/SU638599A1/en

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Description

провод т линейным градиентом NaCE от, 0,1 до 1,5 М с одновременным наложеHHeivi линейного градиента перегнанного-этанола от 0,1 до 48 об.%,a linear gradient of NaCE is conducted from, 0.1 to 1.5 M with simultaneous superposition of HeHivi of a linear gradient of distilled ethanol from 0.1 to 48% by volume,

Объем градиента составл ет 100 мл. Скорость злюции 1 мл/мин. Профили .элюцин регистрируют с поиощью проточного УФ-денситометра (Uvfcond 2LKB ) , Дл  полного вьщелени  второй фракции провод т дополнительную элюцию 5 мл смеси 1 М NaCE в боратном буфере, содержащем 30 об,% диоксана. Далее провод т освобождение фракций отThe volume of the gradient is 100 ml. Zylation rate 1 ml / min. The elucin profiles are recorded with the aid of a flow-through UV densitometer (Uvfcond 2LKB). For the complete separation of the second fraction, an additional elution is carried out with 5 ml of a mixture of 1 M NaCE in borate buffer containing 30 vol% dioxane. Next, the fractions are released from

Нуклеотидный состав ДНК, фракционируемых на БД-целлюлозе (мол рные проценты)The nucleotide composition of DNA fractionated on DB-cellulose (molar percent)

органических растворителей путем выпаривани  на ротационном испарителе. Дл  освобождени  от солей провод т диализ против дистиллированной воды и необходимого буфера или осаждают дав этанолом.organic solvents by evaporation on a rotary evaporator. To release salts, dialyze against distilled water and the required buffer, or precipitate with ethanol.

Нуклеотидный состав ДНК определ ют хроматографией гидроЛизатов (86% НСООН, , 30 мин) на бумаге ватман № 1 и в тонком слое целЛЮ .ПОЗЫ (FND) . Получают две фракции нативной ДНК, отличных по нуклеотидному составу.The nucleotide composition of DNA is determined by chromatography of hydro-Lysates (86% HCOOL, 30 min) on Whatman paper No. 1 and in a thin layer of cella. POSITIONS (FND). Get two fractions of native DNA, different in nucleotide composition.

Claims (2)

Согласно таблице, равенства и указывают на нативность выделен ных фракций. Дл  фракционировани  использ уют препараты ДНК с высоким гиперхромизмом 35-39%, т.е. нативную. Формула изобретени  Способ получени  фракций нуклеиновых кислот путем пропускани  раствора дезоксирибонуклеиновых кислот (ДНК) через колонку с бензоилированной диэтиламиноэтилцеллюлозой с последующей элюцией фракций в линейном градиенте Nace,o тличающийс  ,что,с целью упрощени  пр9Цесса и улучшени  качества фракций ДНК, элюцию проврд т смесью линейного градиента концентраций NaCE 0,1-1,5 М в бо ратном буфере и линейного градиента этанола в концентрации 0,1-48 об.% с дополнительной элюцией смесью раствора NaCe с концентрацией 1-1,5 М в боратном буфере с диоксаном с концентрацией 28-30 об.%. Источники информации, прин тые во внимание при экспертизе: 1. j.w.seaoi-t .R.B.KeEev.P.L.sinsjieimef, Fract-ioncttion of nucEeic acid anteniOsEai;ed -naptitfjoiitated I(EAE-ceEEueose X MoEBioe . 26, p.537-540, 1967, , According to the table, the equalities and indicate the originality of the isolated fractions. For fractionation, using comfort DNA preparations with high hyperchromism of 35-39%, i.e. native The method of obtaining nucleic acid fractions by passing a solution of deoxyribonucleic acids (DNA) through a column with benzoyl diethylaminoethyl cellulose, followed by elution of the fractions in a Nace linear gradient, which, in order to simplify the process of DNA and improve the quality of the DNA fractions, elute the electrode and the figure of the figure. NaCE concentrations of 0.1–1.5 M in the inward buffer and a linear gradient of ethanol at a concentration of 0.1–48% by volume, with additional elution with a mixture of NaCe solution with a concentration of 1–1.5 M in borate Ufer dioxane at a concentration of 28-30 vol.%. Sources of information taken into account in the examination: 1. jwseaoi-t .RBKeEev.PLsinsjieimef, Fract-ioncttion of acid anteniOsEai; ed -naptitfjoiitated I (EAE-ceEEueose X MoEBioe. 26, p.537-540, 1967, 2.N.A.Caffin,A.Q.Mac1 ineoiv Fractionationof DNA on-benioseate DAE-ceeeutose Anat.Bioctlem.,63,p.442-451(975).2.N.A.Caffin, A.Q.Mac1 ineoiv Fractionationof DNA on-benioseate DAE-ceeeutose Anat.Bioctlem., 63, p.442-451 (975).
SU772506145A 1977-07-11 1977-07-11 Method of obtaining nucleic acid fractions SU638599A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU772506145A SU638599A1 (en) 1977-07-11 1977-07-11 Method of obtaining nucleic acid fractions

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Application Number Priority Date Filing Date Title
SU772506145A SU638599A1 (en) 1977-07-11 1977-07-11 Method of obtaining nucleic acid fractions

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SU638599A1 true SU638599A1 (en) 1978-12-25

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4997927A (en) * 1984-09-13 1991-03-05 Gesellschaft Fur Biotechnologische Forschung Mbh (Gbf) Improved process for the purfication of synthetic oligonucleotides
US5981736A (en) * 1997-06-27 1999-11-09 Life Technologies, Inc. One step device and process for concentration and purification of biological molecules
US8247545B1 (en) * 1991-12-02 2012-08-21 Qiagen Gmbh Device and a process for the isolation of nucleic acids

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4997927A (en) * 1984-09-13 1991-03-05 Gesellschaft Fur Biotechnologische Forschung Mbh (Gbf) Improved process for the purfication of synthetic oligonucleotides
US8247545B1 (en) * 1991-12-02 2012-08-21 Qiagen Gmbh Device and a process for the isolation of nucleic acids
US5981736A (en) * 1997-06-27 1999-11-09 Life Technologies, Inc. One step device and process for concentration and purification of biological molecules

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