SU638599A1 - Method of obtaining nucleic acid fractions - Google Patents
Method of obtaining nucleic acid fractionsInfo
- Publication number
- SU638599A1 SU638599A1 SU772506145A SU2506145A SU638599A1 SU 638599 A1 SU638599 A1 SU 638599A1 SU 772506145 A SU772506145 A SU 772506145A SU 2506145 A SU2506145 A SU 2506145A SU 638599 A1 SU638599 A1 SU 638599A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- dna
- fractions
- nace
- concentration
- nucleic acid
- Prior art date
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- Saccharide Compounds (AREA)
Description
провод т линейным градиентом NaCE от, 0,1 до 1,5 М с одновременным наложеHHeivi линейного градиента перегнанного-этанола от 0,1 до 48 об.%,a linear gradient of NaCE is conducted from, 0.1 to 1.5 M with simultaneous superposition of HeHivi of a linear gradient of distilled ethanol from 0.1 to 48% by volume,
Объем градиента составл ет 100 мл. Скорость злюции 1 мл/мин. Профили .элюцин регистрируют с поиощью проточного УФ-денситометра (Uvfcond 2LKB ) , Дл полного вьщелени второй фракции провод т дополнительную элюцию 5 мл смеси 1 М NaCE в боратном буфере, содержащем 30 об,% диоксана. Далее провод т освобождение фракций отThe volume of the gradient is 100 ml. Zylation rate 1 ml / min. The elucin profiles are recorded with the aid of a flow-through UV densitometer (Uvfcond 2LKB). For the complete separation of the second fraction, an additional elution is carried out with 5 ml of a mixture of 1 M NaCE in borate buffer containing 30 vol% dioxane. Next, the fractions are released from
Нуклеотидный состав ДНК, фракционируемых на БД-целлюлозе (мол рные проценты)The nucleotide composition of DNA fractionated on DB-cellulose (molar percent)
органических растворителей путем выпаривани на ротационном испарителе. Дл освобождени от солей провод т диализ против дистиллированной воды и необходимого буфера или осаждают дав этанолом.organic solvents by evaporation on a rotary evaporator. To release salts, dialyze against distilled water and the required buffer, or precipitate with ethanol.
Нуклеотидный состав ДНК определ ют хроматографией гидроЛизатов (86% НСООН, , 30 мин) на бумаге ватман № 1 и в тонком слое целЛЮ .ПОЗЫ (FND) . Получают две фракции нативной ДНК, отличных по нуклеотидному составу.The nucleotide composition of DNA is determined by chromatography of hydro-Lysates (86% HCOOL, 30 min) on Whatman paper No. 1 and in a thin layer of cella. POSITIONS (FND). Get two fractions of native DNA, different in nucleotide composition.
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU772506145A SU638599A1 (en) | 1977-07-11 | 1977-07-11 | Method of obtaining nucleic acid fractions |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU772506145A SU638599A1 (en) | 1977-07-11 | 1977-07-11 | Method of obtaining nucleic acid fractions |
Publications (1)
Publication Number | Publication Date |
---|---|
SU638599A1 true SU638599A1 (en) | 1978-12-25 |
Family
ID=20717411
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU772506145A SU638599A1 (en) | 1977-07-11 | 1977-07-11 | Method of obtaining nucleic acid fractions |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU638599A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4997927A (en) * | 1984-09-13 | 1991-03-05 | Gesellschaft Fur Biotechnologische Forschung Mbh (Gbf) | Improved process for the purfication of synthetic oligonucleotides |
US5981736A (en) * | 1997-06-27 | 1999-11-09 | Life Technologies, Inc. | One step device and process for concentration and purification of biological molecules |
US8247545B1 (en) * | 1991-12-02 | 2012-08-21 | Qiagen Gmbh | Device and a process for the isolation of nucleic acids |
-
1977
- 1977-07-11 SU SU772506145A patent/SU638599A1/en active
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4997927A (en) * | 1984-09-13 | 1991-03-05 | Gesellschaft Fur Biotechnologische Forschung Mbh (Gbf) | Improved process for the purfication of synthetic oligonucleotides |
US8247545B1 (en) * | 1991-12-02 | 2012-08-21 | Qiagen Gmbh | Device and a process for the isolation of nucleic acids |
US5981736A (en) * | 1997-06-27 | 1999-11-09 | Life Technologies, Inc. | One step device and process for concentration and purification of biological molecules |
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