SU587149A1 - Method of preparing lytic enzymes - Google Patents
Method of preparing lytic enzymesInfo
- Publication number
- SU587149A1 SU587149A1 SU752163334A SU2163334A SU587149A1 SU 587149 A1 SU587149 A1 SU 587149A1 SU 752163334 A SU752163334 A SU 752163334A SU 2163334 A SU2163334 A SU 2163334A SU 587149 A1 SU587149 A1 SU 587149A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- preparing
- lytic enzymes
- booo
- boo
- centrifuging
- Prior art date
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Description
1one
Изобретение относитс к микробиологической промышленности и касаетс получени М51кробиологических препаратов .. The invention relates to the microbiological industry and concerns the production of M51 microbiological preparations.
Известен способ получени литических ферментов путем выращивани продуцента гриба MicfOpoLys рога 434 на питательной среде, содержащей источники углерода и азота в присутстви мйнеральньах солей, с последуюсцим выделением фермента из культуральной хсидкости обработкой ее сульфатом аммони и ацетоном llj .A known method for producing lytic enzymes by growing the producer of the fungus MicfOpoLys horns 434 on a nutrient medium containing carbon and nitrogen sources in the presence of mineral salts, followed by isolating the enzyme from the culture trait by treating it with ammonium sulfate and acetone llj.
Известен также способ получени литических ферментов путем культивировани продуцентов на питательной среде, содержащей основные источники питани , с последующим вьщелением целевого продукта центрифугированием 2There is also known a method for producing lytic enzymes by cultivating the producers on a nutrient medium containing the main sources of nutrition, followed by isolating the target product by centrifuging 2
Однако известные способы не позвол ют получить препараты с высокой активностью .However, the known methods do not allow to obtain preparations with high activity.
С целью повьапени активности препарата по предлагаемому cnocq6y в качестве продуцента используют синеэеленыв водоросли А «aba епа variabiBis или AMacystis ttiduEartS} культивируют их на минеральной среде при освещенности бОО-бООО лк и температуре ЗО-ЗЗ С, полученный после центрифугировани осадок клеток дезинтегрируют, экстрагируют целевой продукт буферным раствором с детергентом Тритон Х-100 и осадают сульфатом аммони .In order to improve the activity of the drug according to the proposed cnocq6y as a producer, blue algae are used in the algae А “aba epa variabiBis or AMacystis ttiduEartS} cultivated on the mineral medium under illumination of BOO-BOOO lx and temperature of ZO-ZS C, obtained after centrifuging the precipitated cells of the BOO-BOOO lx and the temperature of ZO-ZS C, obtained after centrifuging the precipitates of BOO-BOOO lx and the temperature of ZO-ZS C, obtained after centrifuging the precipitated cells with BOO-BOOO lx and temperature of ZO-ZS C, obtained after centrifuging the precipitated cells with BOO-BOOO lx and temperature of ZO-ZS C, obtained after centrifuging the precipitates of BOO-BOOO lx and the temperature of ZO-ZS C, obtained after centrifuging the precipitates of BOO-BOOO lx and the temperature of the OO-Z. the product is buffered with Triton X-100 detergent and precipitated with ammonium sulfate.
Способ осуществл ют следующим образом .The method is carried out as follows.
Пример. Сеют по 5 мл восьмидневной Культуры AwabaeitQ variabifis в колбы, со 100 мл среды следующего состава г/л:Example. Sow 5 ml of eight-day AwabaeitQ variabifis Culture in flasks with 100 ml of medium of the following composition g / l:
Нитрат кали KNOj1,0 .Potassium nitrate KNOj1.0.
Сульфат магни ,25Magnesium sulfate, 25
Дикалийфосфат ,04Dipotassium Phosphate, 04
Хлорное железо PeCEj0,002Ferric chloride PeCEj0.002
Хлорид кальци Calcium Chloride
CaCEj-eHjO0,024CaCEj-eHjO0.024
Цитрат натри 0,165Sodium citrate 0.165
Раствор микроэлементов1 мл.Trace element solution 1 ml.
Раствор микроэлементов имеет следующий состав, г/л:The solution of trace elements has the following composition, g / l:
Борна кислота HjBO 2,86Boric acid HjBO 2.86
Хлорид марганцаManganese chloride
MMCEj-4H20(1,81MMCEj-4H20 (1.81
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU752163334A SU587149A1 (en) | 1975-07-28 | 1975-07-28 | Method of preparing lytic enzymes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU752163334A SU587149A1 (en) | 1975-07-28 | 1975-07-28 | Method of preparing lytic enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU587149A1 true SU587149A1 (en) | 1978-01-05 |
Family
ID=20628825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU752163334A SU587149A1 (en) | 1975-07-28 | 1975-07-28 | Method of preparing lytic enzymes |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU587149A1 (en) |
-
1975
- 1975-07-28 SU SU752163334A patent/SU587149A1/en active
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