SU565936A1 - Strain actinomyces thermovulgaris t-54-producent of fibrinolitic ferment - Google Patents

Description

This invention relates to a microbiopogic production, in particular the production of enzyme preparations. A known strain Ac1 is a knife more omrcofaci LS-., Producing fibrinolytic substances. Fibrinolytic activity of the strain in the development of a complex environment - 16 src. units / ml, on synthetic 147 services. units / ml for 18 hours of incubation of fi brinv plates at 37 ° C. I A known strain has a long development cycle and is not high enough; fnbrinolyticuk) activity; The optimum temperature for the growth of the strain, in which the enzyme produced is less stable under the conditions of the blood flow. The aim of the invention is to eliminate these drawbacks. This is achieved using the strain Scfinohiycas tt1e and ovuEga 6T-54. The Act strain ko m y s e S ik e and mo vu P a TS is derived from the sludge of a hot source located on the gray-earth soil in the region of Yangi-Yul village in the Tashkent region Stumm, Act (Howj / ces ihermovuB ar -ls T-54 is stored in the collection of the Department of Microbiology of the Faculty of Biology, Moscow State University under number 54 and is characterized by the following features: Morphological features: Short spiral with 1-6 not tightly compressed curls., Oval spores, ribbed spores, Cultural agar. Growth is good. Air miielium is white, with age astom is gray or dark gray. The colonies are dense. The medium is not colored, the starch-ammonia medium. The growth is good. The aerial mycelium is from light gray to gray. The colonies are grayish. The medium is not colored. The medium with fish extract, agarova. pigment does not form. Synthetic medium, liquid. Mycelium is homogeneous or in the form of small balls of size O, 2-1, O mm. Medium of color of non-strong tea.

H1nologo-iiokhimsky signs. The eurythemal term {schlny actinomycete with cardinal growth points: maximum 6 ° С, optimum 40-50 ° С, minimum 27 ° С,

{It reduces to nitrites. Starch hydropiauet. Peptonized milk. Gelatin razzhvzhaet. Hydrolysis fibrin, phi inogen, plasma,.

Well assimilates peptone.

Attitude towards sugars. Well absorbs glucose, fructose, maltose, lactose,. Coilose, arabinose, ralenose, galactose, poorly absorbs sucrose.

Attitude to alcohols. Absorbs mannitol, sorbitol, dulcite, inositol.

Relation to organic acids. Absorbs succinate, lactate, citrate.

The intensity of development of the proposed strain is higher than that of the known. Comparative data of the fibrinolytic activity of the enzyme secreted into the medium by the proposed and known strains is presented in the table.

Ass1shogiuse5 Hiet HiovuP) ar siT-54 (proposed strain)

clJHOtTtyces auf eofacifiKs 1LS-B-16 (1st strain)

As can be seen from the table, the proposed strain AciiHom ces tkehmovuEjgar 5; T-4 differs from the known strain by the high productivity of the active fibrinolytic enzyme.

Example 1. Thermophilic actinomycete Ac1 ioshusb5 Ihet-rtfOvuEj ai Sj T-54 video of dry spores removed from the surface of an agar medium of known composition containing g / l: soluble starch 10.0; NaCE-5, O; 0.5, with the help of a bacterial loop, are introduced into a sowing plant of the same composition (50% spores from 1 scoop per 1OO ml of medium) and cultured in a deep way prn pH 7.0-7.2 for 1S-24 hours at 35- 55 ° C in shake flasks with a capacity of 750 ml (volume of the medium is 1OO ml). Then the seed material in an amount of 5% is added to the medium of the same composition and grown the same with the particular one for 18-24 hours at a temperature of 35-55 ° C. After the indicated time has elapsed, the culture liquid contains ZOOO sr. u / ml

The culture fluid is separated from the mycelium using paper filters. Fibrinolytic drug is precipitated from the filtrate with ammonium sulphate at a saturation of 0.6-0.8. The resulting enzyme

190O

3000

147

sixteen

the preparation contains specific activity 2OOO-ZOOO cond. units per 1 mg of protein.

Example 2. Thermophilic actinomycete Actinomyces tkermovuH avis T-54 in the form of dry spores removed from the surface of an agar medium of known composition containing, g / l: soluble starch 10, Oj aCP5.0; peptone 5.0j CaCtjH O 0.5, - using

bacterial loops are introduced into the inoculum of the same composition (50% spores from 1 spit per 1OO ml of medium) and cultured in depth / method at pH 1.0-7.2 for 18-24 hours: at 35-55 ° C in the rocking chair count-f bach with a capacity of 75 O ml (volume of medium OOml). Then, seed in the amount of 5% is introduced into a synthetic medium of known composition, containing, g / l: K N0,1,0 j; NH4) jbC, -l, 0; Na, HP04-l2H20 -1,77;

, 5; Ge304 7HjO-O, 001; SaSbz4,0; soluble starch 2O, 0 and grown in the same way for 2-3 days at. 35-55 C. After the specified time, the culture liquid contains 1900 uel, units / ml.

Claims (1)

The culture fluid is separated from the mycelium using paper L1x filters. The fibrinolytic preparation is precipitated from the filtrate with ammonium sulphate at a level of Infectious DIA 0.6-0.8. The obtained enzyme preparation has an activity of 35OO5500 conv. units per 1 mg of protein. The use of the proposed strain in comparison with known actinomycetes has the following advantages: a short cycle of development; high cultivation temperature j high phy inolitic activity of the farmer produced; increase the yield of fibrinolytic enzyme. Formula invention Strain Actinotnyces tkerhHOVufgoifis T-producer of fibrinolytic enzyme. XP Nits in the collection of the Department of Microbiology, Faculty of Biology, Moscow State University under number 54. Morphological features. Sporoskimi short spiral with 1-6 loosely curly tails. The spores are oval, the shell of the spores is ribbed. Cultural features. Peptone-corn agar. Growth is good. Aerial mycelium is white, with age gray or dark gray. Colonies are dense, the medium is not colored. Starchy-jumbo with; food. Growth is good. Aerial mycelium from light gray to gray. The colonies are grayish. The medium is not colored. Medium with fish extract, agar. Colonies brownish or brown. Mellanoid pigment does not form. Synthetic medium, liquid. Mycelium, homogeneous or in the form of small balls with a size of 0.2-1.0 mm. The medium color is not strong, Physiologist-biochemical characteristics, Eurythemic thermophilic actinomycete with cardinal points of growth; maximum 60 С, optimum 49-50 С, minimum 27 ° С. Nitrates restores to nitrites. Starch hydrolyzes. Peptonized milk. Gelatin thins. Hydrolyzes fibrin, fibrinogen, plasma, albumin. Well assimilates peptone. Attitude towards sugars. Well absorbs glucose, fructose, maltose lactose, xylose, arabinose, rhamnose, galactose, poorly absorbs sucrose. Attitude to alcohols. Absorbs mannitol, sorbitol, dulcet., Inositol. Relation to organic acids. Absorbs succinate, lactate, citrate,