SU559701A1 - A method of making collagen-elastic tubes for vascular plastics - Google Patents
A method of making collagen-elastic tubes for vascular plasticsInfo
- Publication number
- SU559701A1 SU559701A1 SU2015179A SU2015179A SU559701A1 SU 559701 A1 SU559701 A1 SU 559701A1 SU 2015179 A SU2015179 A SU 2015179A SU 2015179 A SU2015179 A SU 2015179A SU 559701 A1 SU559701 A1 SU 559701A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- vascular
- plastics
- solution
- elastic tubes
- vessels
- Prior art date
Links
Description
1one
Р1зобретение относитс к области медицины и касаетс способа изготовлени протезов дл реконструктивной хирургии полых органов .The invention relates to the field of medicine and concerns the method of making prostheses for reconstructive surgery of hollow organs.
Известен способ обработки коллагеновых сосудов pacTBopOiVt фермента фиципа при рН 4-7 с последующим дублением формалином и хромоксидом 1.A known method of treating collagen vessels with pacTBopOiVt enzyme ficci at pH 4-7, followed by tanning with formalin and chromium oxide 1.
Однако известный способ не обеспечивает высокой прочности иолучаемых сосудов и не устран ет иммуногенную активность.However, the known method does not provide high strength of the vessels and does not eliminate the immunogenic activity.
Целью изобретени вл етс устранение иммуногенной активности и повышение прочности сосудов.The aim of the invention is to eliminate the immunogenic activity and increase the strength of the vessels.
Эта пель достигаетс тем, что гетерососуды -обрабатывают слабощелочными растворами .солей, затем ферментными препаратами бактериального происхождени , например терр.чзином , при температуре 38-45°С с послед} ющим иодщелачиванием и дубление провод т диальдегидом крахмала.This pellet is achieved by the fact that hetero vessels are treated with weakly alkaline solutions of salts, then enzymatic preparations of bacterial origin, for example, by terrains at a temperature of 38-45 ° C with subsequent iodization and tanning by starch dialdehyde.
Способ осуществл ют следующим образо.м.The method is carried out as follows.
Свежезабранные гетерососуды обрабатывают вначале растворамь нейтральных солей, например 2-10%-пым раствором NaCI, затем слабощелочных, например 1%-ным раствором бикарбоната натри . После этого сосуды инкубируют в растворах нейтральных бактериальных протеииаз, например в растворе протеррнзииа из культуры Asp. Terricola или протосубтилина из культуры Вас. subtilis, с последующей прпвнвкой коллаген-эластиновому остову гепарина в количестве 0,2-2,0%, хондронтинсульфата в количестве 0,1 -1,0% и дублени в растворе дуб щих альдегидов, например в 0,5-3%-ном растворе формальдег 1да в течение 2-24 ч, 1-5%-ном растворе днальдегида крахмала и других. Обработка гетерососудов в растворах нейтральных и слабощелочных солей способствует Здалеиию больпшнства гюдорастворимых плазменных белков. Полное даленне всех веществ, обусловливаюаиьх аитпгепнзю актнвность, происходит в процессе ферментной обработки. После обработки туйы выдерживают давление до 4 атм.Freshly harvested hetero-vessels are first treated with a solution of neutral salts, for example, 2-10% NaCl solution, then slightly alkaline, for example, 1% sodium bicarbonate solution. After that, the vessels are incubated in solutions of neutral bacterial proteiasis, for example, in a solution of proterrnii from the culture of Asp. Terricola or Protosubtilin from the culture of You. subtilis followed by collagen-elastin backbone of heparin in an amount of 0.2-2.0%, chondrontin sulfate in an amount of 0.1 -1.0% and tanning in a solution of tanning aldehydes, for example, in 0.5-3% formaldehyde solution 1 yes for 2-24 hours, 1-5% solution of unaldehyde starch and others. Processing of hetero vessels in solutions of neutral and weakly alkaline salts contributes to the development of most soluble plasma proteins. The complete distant of all substances, due to the validity of actuation, occurs in the process of enzymatic processing. After processing tuyy maintain pressure up to 4 atm.
Дл ;:о 1данп5; 1убам антнкоагул нтных способностей внутрь lix заливают раствор гепарина с конпентрацпей 1-50 ед/мг. Внещиюю поверхность тубы можно обрабатывать раствором хонсурида с концентрацией 1 - 10мг%.For;: about 1 danp5; For tuba antncoagula abilities, lix is poured heparin solution with concentrates of 1– 50 units / mg. The external surface of the tube can be treated with a solution of honsurid with a concentration of 1–10 mg%.
При использовании гетерососудов, консерв;гров нных в 0,4-2%-ных растворах формальдег да , их первоначально обрабатывают в смесп 1-5 Г;л серной кислоты с 60-70 г/л поварепной солп. После нейтрализации в растворе бикарбоната натрп сосуды инкубируют в растворе нейтральных протепназ приWhen using heterovascular vessels, canned; homogenous in 0.4–2% solution of formaldehyde, they are initially treated in a mixture of 1–5 g; l of sulfuric acid with 60–70 g / l of salty salt. After neutralization in the sodium bicarbonate solution, the vessels are incubated in a solution of neutral protepnases at
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU2015179A SU559701A1 (en) | 1974-03-29 | 1974-03-29 | A method of making collagen-elastic tubes for vascular plastics |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU2015179A SU559701A1 (en) | 1974-03-29 | 1974-03-29 | A method of making collagen-elastic tubes for vascular plastics |
Publications (1)
Publication Number | Publication Date |
---|---|
SU559701A1 true SU559701A1 (en) | 1977-05-30 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU2015179A SU559701A1 (en) | 1974-03-29 | 1974-03-29 | A method of making collagen-elastic tubes for vascular plastics |
Country Status (1)
Country | Link |
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SU (1) | SU559701A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4323358A (en) * | 1981-04-30 | 1982-04-06 | Vascor, Inc. | Method for inhibiting mineralization of natural tissue during implantation |
US4378224A (en) * | 1980-09-19 | 1983-03-29 | Nimni Marcel E | Coating for bioprosthetic device and method of making same |
-
1974
- 1974-03-29 SU SU2015179A patent/SU559701A1/en active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4378224A (en) * | 1980-09-19 | 1983-03-29 | Nimni Marcel E | Coating for bioprosthetic device and method of making same |
US4323358A (en) * | 1981-04-30 | 1982-04-06 | Vascor, Inc. | Method for inhibiting mineralization of natural tissue during implantation |
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