SU552353A1 - The method of growing microorganisms - Google Patents

The method of growing microorganisms

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Publication number
SU552353A1
SU552353A1 SU2098678A SU2098678A SU552353A1 SU 552353 A1 SU552353 A1 SU 552353A1 SU 2098678 A SU2098678 A SU 2098678A SU 2098678 A SU2098678 A SU 2098678A SU 552353 A1 SU552353 A1 SU 552353A1
Authority
SU
USSR - Soviet Union
Prior art keywords
microorganisms
yeast
growing microorganisms
electrostatic field
cells
Prior art date
Application number
SU2098678A
Other languages
Russian (ru)
Inventor
Мая Хажетдиновна Шигаева
Тулемеш Оспанович Раманкулов
Владимир Павлович Краденов
Людмила Васильевна Родионова
Original Assignee
Институт Микробиологии И Вирусологии Ан Казахской Сср
Зональное Конструкторское Бюро По Созданию Машин С Применением Электронноионной Технологии Министерства Сельского Хозяйства Казахской Сср
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Application filed by Институт Микробиологии И Вирусологии Ан Казахской Сср, Зональное Конструкторское Бюро По Созданию Машин С Применением Электронноионной Технологии Министерства Сельского Хозяйства Казахской Сср filed Critical Институт Микробиологии И Вирусологии Ан Казахской Сср
Priority to SU2098678A priority Critical patent/SU552353A1/en
Application granted granted Critical
Publication of SU552353A1 publication Critical patent/SU552353A1/en

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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

туру направл ют на выращивание известными способами.The tour is directed to cultivation by known methods.

Напр женность электрического пол  регулируют изменением величины подаваемого напр жени .The intensity of the electric field is controlled by varying the magnitude of the applied voltage.

Пример 1. Воздействию электростатического пол  напр женностью 6 кв/см при времени обработки 5 мин подвергали суспензию кормовых дрожжей Candida tropicalis штамм СК-4. Контролем служили дрожжи, не подвергавшиес  электрообработке. Культивирование проводили на качалке при 180 об/мин и 29-30°С. Количество выросших дрожжевых клеток определ лось в камере Гор ева непосредственным подсчетом клеток под микроскоиом .Example 1. An electrostatic field with a strength of 6 kV / cm at a treatment time of 5 minutes was subjected to suspension of fodder yeast Candida tropicalis strain SK-4. Yeast that was not subjected to electrotreatment served as control. Cultivation was carried out on a rocking chair at 180 rpm and 29-30 ° C. The number of grown yeast cells was determined in the Gorev chamber by direct counting of cells under a microscope.

Таблица 1Table 1

Динамика роста дрожжевых клеток показана в табл. 1.Dynamics of growth of yeast cells is shown in table. one.

Пример 2. Воздействию электростатического пол  напр женностью 7 кв/см подвергали гомогенезированную споровую суспензию культуры Actinomyces coelicolor. Обработанную в электростатическом поле и разведенную суспензию актиномицетов высевали в чашки Петри поверхностным способом на агаризованную питательную среду с последуюш,им прорашиванием в термостатах при 27-28°С. Количество выросших клеток подсчитывали через 7 суток. Эффект электрообработки виден из данных табл. 2.Example 2. An electrostatic field of 7 kV / cm was subjected to a homogenized spore suspension of an Actinomyces coelicolor culture. Processed in an electrostatic field and diluted suspensions of actinomycetes were sown in Petri dishes by surface method on agar nutrient medium followed by sprouting in thermostats at 27-28 ° C. The number of grown cells was counted after 7 days. The effect of electromachining is visible from the data table. 2

Предлагаемый способ активировани  выращивани  микроорганизмов был апробирован в производственных услови х с помошью опытного образца установки (см. фиг. 2).The proposed method for activating the cultivation of microorganisms was tested under production conditions with the help of a prototype plant (see Fig. 2).

Если по прин той технологии биомасса дрожжей в большой дрожжанке увеличивалась на 15 г/л или на 8,6% (с 175 до 190 г/л), то после электрообработки - на 47 г/л или на 34,5% (с 136 до 183 г/л). В дрожжерастительном чане ио прин той технологии биомасса увеличивалась на 27 г/л или на 18% (с 150 до 177 г/л), а при электрообработке - на 53 г/л или на 35,2% (с 151 до 204 г/л).If, according to the technology adopted, the yeast biomass in a large yeast increased by 15 g / l or by 8.6% (from 175 to 190 g / l), after electrical treatment it increased by 47 g / l or by 34.5% (from 136 up to 183 g / l). In the yeast-growing tank of adopted technology, biomass increased by 27 g / l or 18% (from 150 to 177 g / l), and during electrical treatment, by 53 g / l or 35.2% (from 151 to 204 g / l).

Таблица 2table 2

Claims (1)

Формула изобретени  Способ выращивани  микроорганизмов, 55 предусматривающий воздействие на суспензию микроорганизмов электрического пол , отличающийс  тем, что, с целью ускорени  роста микроорганизмов, используют электростатическое поле напр женностью 1 - 7 кв/см, при этом врем  воздействи  составл ет от 3 сек до 30 мин.Claims 55 A method of growing microorganisms, 55 which involves influencing a suspension of microorganisms with an electric field, characterized in that, in order to accelerate the growth of microorganisms, an electrostatic field of intensity 1-7 kV / cm is used, the exposure time being from 3 seconds to 30 minutes .
SU2098678A 1975-01-16 1975-01-16 The method of growing microorganisms SU552353A1 (en)

Priority Applications (1)

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Application Number Priority Date Filing Date Title
SU2098678A SU552353A1 (en) 1975-01-16 1975-01-16 The method of growing microorganisms

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SU552353A1 true SU552353A1 (en) 1977-03-30

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989003876A1 (en) * 1987-10-21 1989-05-05 Biosyn-R Corporation Method for producing cells
DE4142244A1 (en) * 1991-12-20 1993-06-24 Tramm Werner Sabine Dipl Biol Acceleration of enzymatic processes - by exposing the enzyme substrate combination to alternating electric field of fixed or variable frequency
US5888791A (en) * 1996-01-31 1999-03-30 Ipr Institute For Pharmaceutical Research Ag Method of producing bacteriorhodopsin and carotenoids by electrostatic treatment of Halobacterium halobium

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989003876A1 (en) * 1987-10-21 1989-05-05 Biosyn-R Corporation Method for producing cells
DE4142244A1 (en) * 1991-12-20 1993-06-24 Tramm Werner Sabine Dipl Biol Acceleration of enzymatic processes - by exposing the enzyme substrate combination to alternating electric field of fixed or variable frequency
US5888791A (en) * 1996-01-31 1999-03-30 Ipr Institute For Pharmaceutical Research Ag Method of producing bacteriorhodopsin and carotenoids by electrostatic treatment of Halobacterium halobium
US6238663B1 (en) 1996-01-31 2001-05-29 Institute Of Pharmaceutical Research Ag Method for treating hyperkeratotic disease with a Halobacterium halobium lysate

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