SU539554A1 - The method of extraction of glutelins - Google Patents
The method of extraction of glutelinsInfo
- Publication number
- SU539554A1 SU539554A1 SU2090265A SU2090265A SU539554A1 SU 539554 A1 SU539554 A1 SU 539554A1 SU 2090265 A SU2090265 A SU 2090265A SU 2090265 A SU2090265 A SU 2090265A SU 539554 A1 SU539554 A1 SU 539554A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- glutelins
- urea
- alkali
- extraction
- material under
- Prior art date
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- Peptides Or Proteins (AREA)
Description
Изобретение Относитс к физико-химическим способам исследовани запасных белков зерна пшеницы и чмен и может быть использовано дл аналитического и препаративного диск-электрофореза, при изучении компонентного и аминокислотного состава глютелинов .The invention relates to physicochemical methods for studying the storage proteins of wheat and barley grain and can be used for analytical and preparative disc electrophoresis when studying the component and amino acid composition of glutelins.
Известны способы извлечени глютелинов слабыми растворами кислот или щелочей после удалени альбуминов, глобулинов, проламинов или после разделени фракций бел.ков гель-фильтрацией 1.Methods are known for extracting glutelins with weak solutions of acids or alkalis after removing albumin, globulins, prolamins, or after separating the protein fractions by gel filtration 1.
Известен также способ экстракции глютелинов кислой мочевиной с добавкой детергентов - уксусной кислоты, мочевины, цетил триметиламмони бромида с последуюпдей гель-фильтрацией и осаждением сернокислым аммонием 2.Also known is the method of extraction of glutelins with acid urea with the addition of detergents — acetic acid, urea, cetyl, trimethylammonium bromide, followed by gel filtration and precipitation with ammonium sulphate 2.
Однако известный способ выделени глютелинов нродолжителен и трудоемок.However, the known method of isolating glutelins is long and laborious.
Целью изобретени вл етс ускорение процесса .The aim of the invention is to accelerate the process.
Это достигаетс тем, что из исследуемого материала предварительно удал ют неглютелиновые белки, а затем экстрагируют глютелины щелочной мочевиной, причем мочевину со щелочью примен ют в следующем соотнощении компонентов, мол. %:This is achieved by eliminating non-gluten proteins from the material under investigation, and then glutelins are extracted with alkaline urea, and urea with alkali is used in the following ratio of components, mol. %:
Мочевина96,35-97,18Urea96,35-97,18
Щелочь2,82- 3,65Alkalis2,82- 3,65
Способ осуществл ют следующим образом. 1 г муки обрабатывают 10 мл смеси н-бутанол/вода (4:1) в течение 30 мин при комнатной темнературе, при этом материал обезжириваетс , затем экстрагируют неглютелиновые белки последовательным воздействием 0,15 Мраствором хлористого натри , 70%-ным этанолом, 0,05 М раствором уксусной кислоты и 0,1 М раствором уксусной кислоты с 3 М раствором мочевины в течение 1 часа при соотнощении мука : раствор 1 : 10, после чего глютелины извлекают обработкой 5 мл смеси 0,1 М раствора едкого натра с 3 М мочевины , вз тых в соотнощении, соответственноThe method is carried out as follows. 1 g of flour is treated with 10 ml of n-butanol / water mixture (4: 1) for 30 minutes at room temperature, the material is defatted, then extracted with non-lutein proteins with a successive exposure to 0.15 Sodium chloride solution, 70% , 05 M solution of acetic acid and 0.1 M solution of acetic acid with 3 M solution of urea for 1 hour at a flour: solution ratio of 1: 10, after which the glutelins are extracted by treating 5 ml of a mixture of 0.1 M sodium hydroxide solution with 3 M ureas taken respectively
3,65:96,35.3.65: 96.35.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2090265A SU539554A1 (en) | 1974-11-22 | 1974-11-22 | The method of extraction of glutelins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2090265A SU539554A1 (en) | 1974-11-22 | 1974-11-22 | The method of extraction of glutelins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU539554A1 true SU539554A1 (en) | 1976-12-25 |
Family
ID=20605435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU2090265A SU539554A1 (en) | 1974-11-22 | 1974-11-22 | The method of extraction of glutelins |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU539554A1 (en) |
-
1974
- 1974-11-22 SU SU2090265A patent/SU539554A1/en active
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