SU531525A1 - Bone Preservative - Google Patents

Description

cardboard. The first layer of the agent, 1.5-2.0 cm thick, is poured into the autoclaving molds with modified Janet Snane. After the first layer has been ground (for 65% concentration at + 20 ° C for 10-15 min), the prepared transnant is placed on it. In order to avoid transplant precipitation, the prophylaxis should be delayed by placing the graft in the following order: first pour the first layer of briquette and store such molds under sterile grapes, pick up and prepare the graft, then put it in the second layer of this tool.

The second layer is applied to the folded graft at a temperature of 30–35 ° C so that a layer of adhesive around the transplant is less than 1 cm thick.

After pouring the second layer, the molds with glue are placed in a domestic refrigerator, where the briquettes will finally frost, dry and store. After a few hours of being in the refrigerator at –4 ° C, the briquettes acquire a rubbery texture. To speed up the drying, the paper form is removed. Glued paper layer is easily removed with fingers under running water. The marking of briquettes is done in the usual way - by gluing a label indicating the nature of the graft, its number, the date of collection, the surname of the graft and its blood group. Decomposed briquettes are stored in this form for 1-2 months. During this time, their knots occur with a loss of 5-10% moisture, which gives briquettes greater strength. Longer storage makes them even tougher. When stationary storage is excessive. The strength of the brakes is excessive. 1-2 months after pouring, they are stored in the same refrigerator, but in an unsealed plastic bag, which provides a gradual easy shodushivanie, which eliminates excessive durability.

The optimal storage period for bone homotransplants in bone-adhesive briquettes is 1.5-2 years.

Remove the graft from the briquette as follows.

Preliminary, the surface of the briquette is sterilized by treating it in a diocide solution (1: 5000 - solution for treating the hands of the surgeon) for 30 minutes under room conditions. A triple solution and a solution of antibiotics (1 mln. Units of pin flip and 1 g of streptom: 11 skips pa 1000.0 ml of water) can be used for a longer exposure.

When treated with an antiseptic solution, the briquette is heated, once: its outer crust is smoked, and oii is easily cut off;

It is easy to remove the graft from the adhesive briquette. Glue used brnketa can be applied for conservation again.

The transplant, coperped in the bone marrow, does not require thorough cleaning from the glue. The graft extracted from the briquette is subjected to bacteriological testing and treatment for 30 minutes in an antibiotic solution. After that, it can be transplanted to reciprocation.

The proposed means for preserving bone tissue ensures a longer preservation of the biological half -ness of end-of-life transplants, which, due to an increase in their shelf life, allows for the creation of large stores of bone-plastic material, which is necessary for practical surgery.

Form u l a and 3 o b e te p

An agent for preserving bone tissue, characterized in that, in order to improve the biotic state and increase the shelf life of the graft, it contains bone-glue, boric acid and water under the following condition, i.e., relative to: %:

Osteous table ry adhesive 58-72,5

Boric acid3-3,6

Rest.

Water

Sources of information taken into account in the examination:

1. A.P. Padeia, Oboshovappe and tehpika copserver in paraffin tissue transplants of the connective tissue series in the book. New methods for diagnosing the treatment and prevention of major diseases, Moscow, 1961 (irotrotin).