SU518518A1 - Method for determining antigens or antibodies - Google Patents
Method for determining antigens or antibodiesInfo
- Publication number
- SU518518A1 SU518518A1 SU2037331A SU2037331A SU518518A1 SU 518518 A1 SU518518 A1 SU 518518A1 SU 2037331 A SU2037331 A SU 2037331A SU 2037331 A SU2037331 A SU 2037331A SU 518518 A1 SU518518 A1 SU 518518A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- antibodies
- determining antigens
- antigens
- determining
- serum
- Prior art date
Links
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- Investigating Or Analysing Biological Materials (AREA)
Description
(54) С1ЮСОБ ОПРЕДЕЛЕНИЯ АНТИГЕНОВ (1ЛИ АНТИТЕЛ(54) SUBSTANCE TO DEFINIT ANTIGENS (1 OR ANTIBODIES
33
20 мм. Результат учитывают после выде } рани чашек в течение ночи при 37°С.20 mm. The result is taken into account after the discharge of cups overnight at 37 ° C.
Реакци СИАРФ вы вл ет антитепа Щ)1(1, раз&едении гемолитической сыворот- i(. : 16000 (3, БОЕ в опытном элюате, менее. 10 в контрольном), в то врем как реакци геммагглютинации была положительной в разведении 1:1000 Таким образом, СИАРФ может быть использована дл обнаружени протйвоклеТрчных антител.The SIAPF reaction reveals an antitope S) 1 (1, hemolytic serum i & i (.: 16000 (3, PFU in the experimental eluate, less than. 10 in the control), while the hemagglutination test was positive at 1: 1000 Thus, SIARF can be used to detect protivocellular antibodies.
Пример 2, Фибрин из крови лошади коньюгирует с диазотированной паминобензойной кислотой (АСБ), Конью- гат смешивают с сефадексом (мелкий ) в соотношении 1: 9 и загружают в колонки диаметром 5 и высотой 15 мм. Антитела сорбируют из сыворотки кролика , иммунизированного коньюгатом Г1А1 БСА (бычий сывороточный альбумин) с помощью колоночной техники, Последова518518Example 2 Fibrin from horse blood conjugates with diazotized paminobenzoic acid (ASB), Conjugate is mixed with Sephadex (fine) in a ratio of 1: 9 and loaded into columns with a diameter of 5 and a height of 15 mm. Antibodies are sorbed from rabbit serum immunized with G1A1 BSA conjugate (bovine serum albumin) using column techniques, Sledov518518
тельность операций, состав разбавител и реагентов аналогичны примеру 1.The complexity of the operations, the composition of the diluent and reagents are similar to example 1
Реакци СИАРФ вьшвл ет антитела при разведении айтисьгоороткй 1:1000 (4,1 10 БОЕ в опытном эшоатё, 7,5 10 в контрольном), в то врем как реакци пассивной гемагглютинации была положительна в разведении 1:80. Таким образом, СИАРФ может быть использована дл обнаружени противогаптенных антител ,The SIAP reaction responded to antibodies at a dilution of 1: 1000 (4.1 10 PFU in an experimental echoate, 7.5 10 in the control), while the passive hemagglutination test was positive at a 1:80 dilution. Thus, SIAP can be used to detect anti-captive antibodies,
Пример 3. Бычий сывороточтный альбумин (БСА) коньюгируют с сефадексом Г-75( сверхмелким) с помощью сернокислого эфира бета-оксизтилсульфа ниланилина по известному способу. Антитела сорбируют из кроличьей анти-ПАВEjCA сыворотки с определением по предлагаемому способу (пример 2), Данные представлены в таблице.Example 3. Bovine serum albumin (BSA) is conjugated to Sephadex G-75 (ultrafine) with beta-oxystilsulfonyl nilaniline sulfate ester by a known method. Antibodies are absorbed from rabbit anti-PAVEjCA serum with the definition of the proposed method (example 2). The data are presented in the table.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU2037331A SU518518A1 (en) | 1974-06-26 | 1974-06-26 | Method for determining antigens or antibodies |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SU2037331A SU518518A1 (en) | 1974-06-26 | 1974-06-26 | Method for determining antigens or antibodies |
Publications (1)
Publication Number | Publication Date |
---|---|
SU518518A1 true SU518518A1 (en) | 1976-06-25 |
Family
ID=20588805
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SU2037331A SU518518A1 (en) | 1974-06-26 | 1974-06-26 | Method for determining antigens or antibodies |
Country Status (1)
Country | Link |
---|---|
SU (1) | SU518518A1 (en) |
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1974
- 1974-06-26 SU SU2037331A patent/SU518518A1/en active
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