SU457475A1 - The method of producing prostaglandins - Google Patents
The method of producing prostaglandinsInfo
- Publication number
- SU457475A1 SU457475A1 SU2011152A SU2011152A SU457475A1 SU 457475 A1 SU457475 A1 SU 457475A1 SU 2011152 A SU2011152 A SU 2011152A SU 2011152 A SU2011152 A SU 2011152A SU 457475 A1 SU457475 A1 SU 457475A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- evaporated
- producing prostaglandins
- prostaglandins
- dissolved
- extracted
- Prior art date
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
(54) СПОСОБ ПОЛУЧЕНИЯ ПРОСТАГЛАНДИНОВ(54) METHOD FOR OBTAINING PLASTICLANDINS
Биосинтез осуществл ют следующим образом .Biosynthesis is carried out as follows.
100 мг арахидоновой кислоты смешивают с 0,5 г восстановленного глютатиона и 100 г гомогената , полученного измельчением 100 г пузырьковых желез барана (без соприкосновени с металлом) в 200 мл 0,15 М фосфатного буфера (рН 8,4) и инкубируют аэробно при перемешивании без доступа пр мых лучей света в течение 30 мин при 37°С. После этого в инкубируемую смесь добавл ют еще 100 г того же гомогената, в котором раствор ют 0,5 г восстановленного глютатиона. Через 30 мин биосинтез прерывают добавлением к инкубату 1800 мл этилового спирта, и смесь фильтруют; фильтрат упаривают в вакууме при температуре не выше 35°С до содержани спирта в смеси 66% и экстрагируют равным объемом петролейного эфира. Эфирный раствор упаривают в вакууме до сухого состо ни . Сухой остаток раствор ют в 10 мл метанола, 0,1 мл метанольного раствора хроматографируют на пластинке с силикагелем в системе бензол - диоксан - уксусна кислота в соотношении 20:20: 1. Зону с Rf 0,62-0,64 элюируют метанолом оптически чистым, спектрофотометрируют и определ ют максимум поглощени при 278 нм. Биологическа активность водного раствора элюата соответствует активности простагландина Е2.100 mg of arachidonic acid are mixed with 0.5 g of reduced glutathione and 100 g of homogenate obtained by grinding 100 g of vesicle ram glands (without contact with metal) in 200 ml of 0.15 M phosphate buffer (pH 8.4) and incubated aerobically with stirring without access of direct light rays for 30 min at 37 ° C. Thereafter, another 100 g of the same homogenate is added to the incubated mixture, in which 0.5 g of reduced glutathione is dissolved. After 30 min, biosynthesis is interrupted by adding to the incubate 1800 ml of ethyl alcohol, and the mixture is filtered; the filtrate is evaporated in vacuum at a temperature not higher than 35 ° C until the alcohol content in the mixture is 66% and extracted with an equal volume of petroleum ether. The ether solution is evaporated in vacuo to dryness. The dry residue is dissolved in 10 ml of methanol, 0.1 ml of the methanol solution is chromatographed on a silica gel plate in the benzene - dioxane - acetic acid system at a ratio of 20:20: 1. The zone with Rf 0.62-0.64 is eluted with methanol optically pure , spectrophotometrically and determine the maximum absorption at 278 nm. The biological activity of the aqueous solution of the eluate corresponds to that of prostaglandin E2.
Предмет изобретени Subject invention
Способ получени простагландинов путем ферментативного превращени ненасыщенных высших жирных кислот в буферном растворе, например фосфатном, и экстрагировани петролейным эфиром, отличающийс тем,A method for producing prostaglandins by enzymatically converting unsaturated higher fatty acids in a buffer solution, such as phosphate, and extracting it with petroleum ether, characterized in
что, с целью расширени сырьевой базы, отходы производства препаратов надпочечников и поджелудочной железы экстрагируют этиловым спиртом, фильтруют, к фильтрату добавл ют щелочь, серную кислоту, экстрагируют бензолом, упаривают, остаток раствор ют в ацетоне, провод т низкотемпературную кристаллизацию, ацетон упаривают, остаток хроматографируют с последующей ферментацией известными приемами.that, in order to expand the raw material base, the waste products of the adrenal and pancreas preparations are extracted with ethyl alcohol, filtered, alkali, sulfuric acid is added to the filtrate, extracted with benzene, evaporated, the residue is dissolved in acetone, low-temperature crystallization is performed, acetone is evaporated, the residue is evaporated chromatographic followed by fermentation by known methods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2011152A SU457475A1 (en) | 1974-04-12 | 1974-04-12 | The method of producing prostaglandins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU2011152A SU457475A1 (en) | 1974-04-12 | 1974-04-12 | The method of producing prostaglandins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU457475A1 true SU457475A1 (en) | 1975-01-25 |
Family
ID=20580435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU2011152A SU457475A1 (en) | 1974-04-12 | 1974-04-12 | The method of producing prostaglandins |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU457475A1 (en) |
-
1974
- 1974-04-12 SU SU2011152A patent/SU457475A1/en active
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