SU450203A1 - Device for reading and determining the number of micro-objects - Google Patents

Description

one

This invention relates to the field of automation, in particular, to a device for the automatic analysis of micro-objects and structures.

Devices for automatic counting of micro-objects are known, containing a scanning microscope, two delay lines, a chord extraction unit, a counter, a micro-object end detection unit and a control unit.

A disadvantage of the known devices is that they cannot provide high precision counting of micro-objects with cavities in the outer contour and holes.

The purpose of the image; the retention is to improve the accuracy of counting micro-objects of arbitrary shape.

The goal is achieved by using the opening start detection block, the opening end detection block, the trigger and the OR scheme, which allows you to select and memorize the beginning of the microobject (object) hole, select the end of the microobject hole and add the index of the start hole of the microobject to the delay block. This index, originating at the output of the delay unit, establishes the TpBr-reip unit, the signal of which, through the OR circuit, replaces the chord in the gap at the hole site.

Thus, with the fictitious chords, all the openings and depressions along the external contour of the microobjects are directed towards the previous scan lines, and false situations of the end of the microobject are established. As a result of this transformation, for each of the micro-objects of arbitrary shape, only one situation is identified as the end of the micro-object, having calculated that it is not difficult to determine the number of micro-objects.

The drawing shows a block diagram of the proposed device.

The device consists of the line zde | rzhki 1, the scheme "OR 2, trigger 3, block 4 to determine the beginning of the hole of the micro-object, block

method of determining the end of the hole of the micro-object, control unit 6, delay unit 7, block

8determine the end of the micro-object, the counter

9 and a scanning microscope 10.

The output of the microscope 10 is connected to the input of the delay line 1, with one of the inputs of the block 4 for determining the beginning of the opening of the micro-object, block 5 for determining the end of the opening of the micro-object and block 8 for determining the end of the micro-object. The delay line 1 is connected to the input of the OR 2 circuit and to the zero input of flip-flop 3, and the OR circuit to the other inputs of the microbject 4 and 5 detecting units and 5, as well as to the second input of the microbject end measuring unit 8.

The unit 4 for determining the start of the microbject hole is connected to the microbject opening end detection unit 5 and the control unit b, and the output of the microbject opening end detecting unit is connected to another input of the control unit 6 connected to the delay unit 7, the output of which is connected to the trigger unit 3 input.

The output of the trigger 3 is connected to the second input of the Circuit OR 2, and the output of the End Object Definition 8 block of the micro-object is connected to the input of the counter 9.

Delay line 1 and delay unit 7 provide a delay time equal to the scan time per line. The delay unit has many inputs and allows you to enter information at any point from the beginning to the end of the delay.

Block 4 of determining the beginning of a microbject hole selects the Situation "beginning of a hole (" BUT) ", i.e., the point in time when the chord of the current scan line ends at: 1: | the parent line of the previous line.

Block 5 of determining the end of a microbject hole outlines the situation of the end of a borehole (QO), i.e., a point in time, When the chord starts on the current scan line while the previous line of the previous line and the presence of a signal from block 4 of the start of a microbject opening is present.

Block 8 of determining the end of the micro-object highlights the situation of the end of the micro-object ("CM"), i.e., establishes the fact that there is no chord on the current scan line for the entire row of the previous line. The device works as follows. The video pulses of the current scan line come from the output of the scanning microscope 10 to the input of the delay line 1 and to the first INPUT of the micro-object end detection unit 8. At the output of the line of delay | 1, video pulses of the previous scan line appear, which, having passed the OR 2 scheme, go to the second input of the block 8 determining the end of the micro-object. Highlighting the situation “KM, this block generates a signal to the input of counter 9. Thus, by completing the number of situations”, the KM calculates the Number of Scanned Objects.

If the previous line breaks the chord on the current scan line, block 4 starts detecting the opening of the micro-object and issues a signal "BUT to the third input of the block determining the end of the micro-object and to the first input of the control unit 6. Block 5 triggers when the same chord of the previous line again through the chords on the current scan line.

The signal "KO from the output of block 5 goes to the second input of the control unit 6, which monitors the advancement of the beginning of the hole along the delay line 1, having received a SIGNAL" BUT from 5 outputs of block 4.

The signal "KO control unit 6 records the index of the beginning of the hole in such a point of the delay unit 7, which corresponds to the point of the beginning of the hole in the line

0 delay 1. Thus, the index of the beginning of the hole is at the output of the delay unit at the moment when the output of the delay line 1 is broken by the chord of the previous scan line. The index of the beginning of the hole from the output of block 7 enters the single input of the trigger 3, transferring it to the state of unity. The output signal from trigger 3 passes through the "OR 2" circuit and replaces the chord break section on the previous scan line with a fictitious chord segment. As soon as the discontinuity ends and a chord appears again at the output of the delay line 1, the trigger 3 is reset by the signal from the output of the delay line 1 to

5 zero.

Consequently, broken chords of one object are represented. at the output of the circuit “OR 2 as one whole chord. As a result of such a transformation, the openings and depressions of the object's outer contour are turned into the previous scanning lines, which eliminates false situations of the CM and allows automatic counting of objects of arbitrary shape by counting the number of true situations of the CM.

Subject invention

0 A device for reading and determining the number of micro-objects, containing a scanning microscope, connected to the unit for determining the end of the micro-object directly and through serially connected

5 delay line and OR circuit, a counter connected to the micro-object end detection unit, a control unit connected to the delay unit, and a trigger, characterized in that, in order to improve the micro-objects counting accuracy, the microbject hole End definition block, connected to a scanning micrograph, an OR circuit, and a control unit, a micro-object opening start detection unit connected to a micro-object opening end detection unit and a control unit, with the trigger inputs (pa connected to the delay line and a delay unit, and the output is connected to another circuit input

"OR.