SU269889A1 - METHOD OF OBTAINING AMYLOLYTIC ENZYME PREPARATIONS - Google Patents
METHOD OF OBTAINING AMYLOLYTIC ENZYME PREPARATIONSInfo
- Publication number
- SU269889A1 SU269889A1 SU1246959A SU1246959A SU269889A1 SU 269889 A1 SU269889 A1 SU 269889A1 SU 1246959 A SU1246959 A SU 1246959A SU 1246959 A SU1246959 A SU 1246959A SU 269889 A1 SU269889 A1 SU 269889A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- enzyme preparations
- obtaining
- amylolytic enzyme
- enzymes
- extract
- Prior art date
Links
- 230000003625 amylolytic Effects 0.000 title description 6
- 229940079919 digestives Enzyme preparations Drugs 0.000 title description 5
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 229940110715 ENZYMES FOR TREATMENT OF WOUNDS AND ULCERS Drugs 0.000 description 5
- 229940088598 Enzyme Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 229940079593 drugs Drugs 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 102000035443 Peptidases Human genes 0.000 description 2
- 108091005771 Peptidases Proteins 0.000 description 2
- 229940024999 Proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 2
- 239000006286 aqueous extract Substances 0.000 description 2
- 230000001580 bacterial Effects 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N iso-propanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000007792 addition Methods 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Description
Р1зобретение относитс к области ферментной промышленности и представл ет собой способ получени амилолитических ферментных препаратов.The invention relates to the field of the enzyme industry and is a method for the preparation of amylolytic enzyme preparations.
Нзвестны способы получени амилолитических ферментпых препаратов путем поверхностного выраш,ивани их продуцентов, например Вас. Subtil is, с последующим выделением ферментов из клеток последних.Methods for the preparation of amylolytic enzyme preparations by surface growth, such as yours, are known. Subtil is, followed by the release of enzymes from the cells of the latter.
Продуценты экстрагируют водой и из полученной водной выт жки выдел ют ферменты путем осал ;дени их органическими растворител ми .Producers are extracted with water and the resulting aqueous extract is isolated enzymes by sedi and their organic solvents.
РГзвестные способы получени бактериальной а-амилазы св заны с трудоемким процессом концентрировани ферментной выт жки под вакуумом с добавлением CaCU.EH known methods for producing bacterial a-amylase are associated with a time-consuming process of concentrating an enzyme extract under vacuum with the addition of CaCU.
Предлагаемый способ получени амилолитических ферментных препаратов позвол ет получить их из поверхностной культуры.The proposed method for the preparation of amylolytic enzyme preparations allows them to be obtained from surface culture.
Дл инактивации протеолитических ферментов , одновременно продуцируемых этим же микроорганизмом, предложено экстракт ферментов подвергать термической обработке, которую осуществл ют при температуре 60°С в течение 30-45 мин.To inactivate proteolytic enzymes simultaneously produced by the same microorganism, it was proposed to extract the enzymes by heat treatment, which was carried out at a temperature of 60 ° C for 30-45 minutes.
Экстракт ферментов из бактериального технического препарата Вас. Subtilis готов т настаиванием препарата водопроводной водой комнатной температуры в соотношении 1 : 7 в течение 2 час при перемешивании и отжатииEnzyme extract from bacterial technical preparation You. Subtilis was prepared by infusing the drug with tap water at room temperature at a ratio of 1: 7 for 2 hours while stirring and squeezing.
препарата через марлю. Затем этот экстракт подвергают термической обработке в течение 30-45 мин при температуре 60°С.drug through cheesecloth. Then this extract is subjected to heat treatment for 30-45 minutes at a temperature of 60 ° C.
Осаждение а-амилазы и термически обработанной водной выт жки производитс изопропиловым спиртом 50%-ной концентрации или этанолом 55%-ной концентрации.The precipitation of a-amylase and heat-treated aqueous extract is made with isopropyl alcohol at a 50% concentration or with 55% ethanol.
Применение термической обработки и осаждение изонропнловым спиртом 50%-ной концентрации дают возможность получать препарат со значительным преобладанием амилолитических ферментов. Препарат имеет специальное назначение, его примен ют в малых дозах, приблизительно 0,00004-0,00009% от веса муки, что способствует лучшему сохранению свежести хлеба.The use of heat treatment and the precipitation of 50% concentration by isonopnil alcohol give the opportunity to obtain a drug with a significant predominance of amylolytic enzymes. The drug has a special purpose, it is used in small doses, approximately 0.00004-0.00009% of the weight of the flour, which contributes to a better preservation of the freshness of the bread.
Предмет изобретени Subject invention
1. Способ получени амилолитических ферментных препаратов, например а-амилазы, путем выращивани их продуцентов, например Вас. Subtilis, отделени выращенных клеток с последующей экстракцией и осаждением ферментов органическими растворител ми, отличающийс тем, что, с целью инактивации протеолитических фер.ментов, одновременно продуцируемых этим же микроорганизмом, экстракт ферментов подвергают термической обработке ..1. A method for producing amylolytic enzyme preparations, e.g. a-amylases, by growing their producers, e.g. you. Subtilis, separating the grown cells, followed by extraction and precipitation of the enzymes with organic solvents, characterized in that, in order to inactivate proteolytic enzymes simultaneously produced by the same microorganism, the enzyme extract is subjected to heat treatment ..
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU269889A1 true SU269889A1 (en) |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2368497A1 (en) * | 1976-08-05 | 1978-05-19 | Cpc International Inc | PROCESS FOR SELECTIVE INACTIVATION OF PROTEASE IN A PREPARATION OF BACTERIAL A-AMYLASE, PREPARATION OF ENZYME OBTAINED AND APPLICATION TO HYDROLYSIS OF A STARCHLACE MATERIAL |
| EP0021179A2 (en) * | 1979-06-08 | 1981-01-07 | Deutsche ITT Industries GmbH | Inactivation of protease activity in an enzyme mixture, purification and concentration of the remaining alpha-amylase and bakery products using said purified alpha-amylase |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2368497A1 (en) * | 1976-08-05 | 1978-05-19 | Cpc International Inc | PROCESS FOR SELECTIVE INACTIVATION OF PROTEASE IN A PREPARATION OF BACTERIAL A-AMYLASE, PREPARATION OF ENZYME OBTAINED AND APPLICATION TO HYDROLYSIS OF A STARCHLACE MATERIAL |
| EP0021179A2 (en) * | 1979-06-08 | 1981-01-07 | Deutsche ITT Industries GmbH | Inactivation of protease activity in an enzyme mixture, purification and concentration of the remaining alpha-amylase and bakery products using said purified alpha-amylase |
| EP0021179A3 (en) * | 1979-06-08 | 1982-06-23 | Deutsche ITT Industries GmbH | Inactivation of protease activity in an enzyme mixture, purification and concentration of the remaining alpha-amylase and bakery products using said purified alpha-amylase |
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