SU1740421A1 - Method of obtaining hydrolyzate for growing fodder yeast - Google Patents

Abstract

Usage: hydrolysis industry, method of obtaining fodder yeast. SUMMARY OF THE INVENTION: Waste streams with an excess of activated sludge with a concentration of 70–120 g / l, obtained during the cleaning of the last mash wort with active sludge, are directed at a rate of 6–8 m3 / h to mixing with water in a ratio of 0.2–0.3: 1 and feeding them to hydrolysis. The hydrolysis is carried out at elevated temperature and elevated pressure, followed by neutralization of sulfuric acid, settling, cooling the hydrolyzate, feeding to the fermenter, separating the post-yeast brew from the yeast, cleaning the post-brewing brew with active sludge and returning the effluent with excess active sludge to hydrolysis. 1 tab.

Description

The invention relates to the production of hydrolysates from plant raw materials for the production of fodder yeast.

There is a known method in which excess activated sludge undergoes hydrolysis at t 80–150 ° C using 0.5–10% sulfuric acid with further processing of the resulting hydrolyzate, which after neutralization is sent to fermentation for use in growing various microorganisms: yeast of the genus Candida.

The closest to the present invention is a method of obtaining a hydrolyzate for growing fodder yeast, which consists in grinding vegetable raw materials, supplying the aqueous phase and acid, heating with steam at 140-180 ° C and 5-10 atm pressure, percolating and neutralizing the hydrolyzate, followed by cultivation fodder yeast.

The disadvantages of this process are the low concentration of the substrate RV applied to the fermenter and the deterioration of the economic environment. The latter is due to the fact that after cleaning the yeast brew with active sludge the drains require additional processing, which is associated with economic costs.

The aim of the invention is to increase the utilization of reducing sugars and to improve the environmental friendliness of the process.

The goal is achieved by using a mixture of water with a post-yeast brew, subjected to preliminary purification with activated sludge, with a residual content of 70-120 g / l, with a ratio of components of 0.2

VI

4 o

Yu

- 0.3: 1, while the aqueous phase is fed at a speed of 6 - 8 m / h.

A decrease or increase in the concentration of activated sludge leads to a decrease in residual RV in the mash and a decrease in the percentage of utilization of RV.

The hydrolysis is carried out at elevated temperature (140-180 ° C) and a pressure rise from 5 to 11 atm for 290-300 minutes. The resulting hydrolyzate is neutralized with milk of lime and ammonia water, purification from suspended impurities. The hydrolyzate cooled in the vacuum cooling unit is fed to fermentors for growing yeast, which are separated from the post-yeast brewing at the separation stage and the latter is sent for purification with active sludge.

The effluent from the activated sludge obtained during the cleaning process is directed to mixing with water and the process is repeated. Thus, wastewater is used with an excess of activated sludge in the process of hydrolysis, which significantly improves environmental treatment.

Characterization of effluent with excess activated sludge:

Concentration

active

sludge, g / l 70-120

Temperature,

° С10-15

рН6,4

Color Dark Brown

Oxidation,

mg / l 1000

Ammonia nitrogen,

mg / l200 - 250

Phosphorus (P20s),

mg / l 50 - 60

BPKb, mg / l 90-95

CPC, mg / l900 - 950

Weighted

substances, mg / l 400 - 450

Example, Raw materials (sunflower husk) are sent to the upper neck of the hydrolysis apparatus, and activated sludge is also fed there. On the side of the hydrolysis apparatus, water and acid are fed through the mixer. The activated sludge is set to 3 m3, water -14m, acids depending on the raw material - 80-100 m3. Loading lasts for 30 min. In the upper neck of the hydrolysis apparatus everything is mixed. Hydrolysis apparatus closes

a lid. The mixture is heated for 30 minutes with steam to a pressure of 5 atm, after which percolation begins. The boiling mixture is supplied from the side through a mixer (water and acid), the liquid that is already enriched with sugars, called hydrolyzate, is removed from the bottom of the apparatus. The acid content in it is 0.5%. Percolat Q passes at a pressure of 5 - 11.5 atm.

The hydrolyzate is neutralized, settled, cooled and fed to the cultivation of yeast in fermenters.

The yeast suspension from the fermenter is fed through a flotation cell to the first group.

separation, where it thickens to a concentration of 100-120 g / l. The concentrate enters the collection, from where it is pumped to the second separation group, and the post-harvest wort from the first separation group is pumped to the treatment plant for purification with active sludge. A part of the post-yeast brew, purified by activated sludge (active sludge drains), is sent for mixing with water, returned for loading into the hydrolysis apparatus and the process is repeated.

After the separation group II, the concentrate enters the dryer and the yeast packaging. The indicators of the method are shown in the table (with the content of PB in the wort 50%).

Compared with the prototype, when using wastewater with an excess of activated sludge, fresh river water is saved, and the amount of wastewater directed to utilization per unit of production is reduced. So

the process improves the environmental friendliness and increases the utilization of reducing sugars.

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Claims (1)

Invention Formula A method for producing a hydrolyzate for growing fodder yeast, including grinding plant material, feeding the aqueous phase and acid, heating with steam, percolating and neutralizing the hydrolyzate, characterized in that, in order to increase the utilization of reducing sugars and improve the environmental friendliness of the process, use a mixture water with post-yeast brew, subjected to preliminary purification with activated sludge with a residual content of 70-120 g / l with a ratio of components of 0.2 - 0.3: 1, while the aqueous phase is fed from speed of 6-8 m3 / h.