SU1729352A1 - Method for provoking polyovulation in cows-donors at embryo transfer - Google Patents

Abstract

The invention relates to animal husbandry, in particular, to methods for artificially causing polio stimulation in cows selected as donors of embryos during transplantation. The aim of the invention is to reduce the laboriousness of the method and increase fertilization ™. The method consists in administering a preparation of the following composition: follicle-stimulating hormone of the pituitary 40-50 mg, vitamin A 24, 7-3.4.6 mg, vitamin Dz 2.5-3.5 mg, vitamin 50-70 mg. 10% ethereal solution of lecithin 0.5-1.0 ml or tween 1-2 drops, distilled water 20 ml. The drug is administered intramuscularly for 3 days, one injection per day. On the 1st day, 7 ml was injected, on the 2nd day - 7 ml, on the 3rd day - 6 ml. 2 tab.

Description

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The invention relates to animal husbandry, in particular, to methods for artificially causing polio in cows selected as donors of embryos during transplantation.

There is a known method of inducing poliovulation in cows by a single treatment in the luteal phase of the cycle of gonadotropin in the serum of foals of mares (FGM).

The disadvantage of the PEG method is the low predictability of the method, often a large percentage of animals show prey prematurely, besides, the dosage of this drug is difficult, as a result it can be overdose easily / which leads to ovarian follicular cysts.

The closest to the present invention is a method for inducing -polyovulation by multiple injections (8 - 10

injections over 4–5 days, 2 injections daily for follicle-stimulating hormone of the pituitary gland.

The disadvantage of this method is its laboriousness associated with multiple daily treatments, which often causes stressful effects on highly productive donor cows.

The purpose of the invention is to reduce the complexity of the existing method of preparing donor cows and increase the fertilizing ability of animals.

The goal is achieved by administering gonadotropic drug intramuscularly to cows for 3 days, one injection per day, day 1 and day 2, 7 ml each, and day 3 - 6 ml. As a gonadotropic drug, follicle-stimulating hormone (FSH) is used in the form of an emulsion, in the aqueous phase of which FSH is dissolved, in the oil phase - vitamins A, Dz, E, emulsified

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lecithin or twin is used as the binding agent. Vitamins that are introduced into the drug and enter the body accelerate oogenesis, as a result of which the polyovulatory effect of FSH is enhanced, in addition, the emulsion prolongs the action of FSH. as a result, it is possible to more than halve the number of injections of the drug and the duration of treatment.

The method of manufacture. The emulsion is prepared based on 10 donor cows. To do this, add 200 ml of tri-vitamin and 5-7 ml of a 10% ethereal solution of lecithin or 5-10 drops of tween to a 200-ml chemically pure split glass, add 150-170 ml of distilled water to the mark of 150-170 ml dispersed on an ultrasonic disperser U ZDN-1 at a frequency of 35 kHz, exposure time 1-2 minutes. FSH at the rate of 40-50 mg per dose, 400-500 mg per. 10 doses are dissolved in 30-50 ml of distilled water, transferred into a glass with an emulsion, mixed mechanically and dispersed for an additional 10-20 seconds. In this form, the drug is ready for use, stored at 2 - 5 ° C for up to 3 m.

Method of application. The proposed preparation is administered intramuscularly to induce poliovulation to donor cows during the luteal phase of the cycle (8th to 13th day of the cycle) for 3 days, one injection per day; 1st day - 7 ml, 2nd - 7 ml, 3rd - 6 ml.

48 h after the start of the administration of the preparation, prostaglindine F2alfa was administered at the rate of 500 µg of cloprostenol to synchronize the hunting. For this purpose, synthetic drugs estrophan, extroprost are used. dynaprost, estruphalan, etc. Estrus usually occurs 48 hours after the administration of prostaglandin F2alpha. Insects cows throughout the hunt (2-3 times) with an interval of 12 hours. Embryo washing and transplanting are performed on the 7th-8th day after the end of the hunt.

Testing of the developed drug was carried out at the transplantation points. As prototypes, the preparation of donors with a pure FSH preparation according to the classical scheme was used: 5-day administration with 2 injections daily or the treatment of FGLC with follygone.

Table 1 presents data on the effectiveness of the proposed and known methods.

As can be seen from the data of Table 1, the application of the developed method on 53 donor cows in the luteal phase of the cycle contributed to the induction of poliovulation in 39 (75%)

cows, which is slightly higher result in animals in the group treated with pure FSH. Noticeably higher is the number of cows from which embryos are obtained when processing them using the proposed method as compared to the prototype. Drug administration also increased the number of viable embryos (88%). In the group of cows receiving GSFC, this figure is 79% and in the group receiving FSH, 60%.

One dose of the complex gonadotropic preparation contains 40-50 ml of follicle-stimulating hormone of the pituitary gland (FSH) 24.7 - 34.6 vitamin A; 2.5 - 3.5 mg of vitamin D; 50 to 70 mg of vitamin E; 0.5 - 1.0

ml of 10% ethereal lecithin solution or 1 - 2 drops of tween-20. - The drug after dispersion on an ultrasonic disperser UZDN-1 at a frequency of 35 kHz for 1 to 2 minutes is a white emulsion with a weak

ethereal smell, shelf life of 3 months at 2-5 ° C.

Table 2 shows the composition of the gonadotropic drug.

The composition with the minimum content of ingredients is applied to heifers or

first-calf cows with a live weight up to 400 kg, with an average cow — with a live weight of 400

-500 kg, with a maximum - 500 kg and more.

The proposed drug is administered intramuscularly to induce poliovulation to donor cows during the luteal phase of the cycle (8th to 13th day of the cycle) for three days, one injection per day: 1st day 7 ml; 2nd - 7 ml and 3rd - 6 ml. 48 hours after the start of the administration of the preparation, prrstaglandin F2-alpha was administered at the rate of 500 µg of the synthetic preparation - cloprostenol.

.Characteristics of ingredients. Follicle-stimulating hormone pituitary FSH-lyophilically dried, white, odorless

powder, well soluble in 0.9%

NaCl solution. Store at temperature o

-4 ° C throughout the year.

Vitamin A crystals, white or pale yellow in color with a faint odor, quickly decompose in air; soluble in alcohol, chloroform, ether and fatty oils, insoluble in water. Vitamin A acetate solution in oil may be used: 3.44; 6.88; 8.6% (clear oil on the liquid without a rancid odor and taste).

A solution of vitamin D3 in oil is a clear, oily liquid from light yellow to dark yellow without a rancid odor.

Vitamin E is a light yellow clear viscous liquid with a faint odor. It oxidizes and darkens in the light. Practically insoluble in water, soluble in alcohol, acetone, ether and vegetable oils.

Trivitamin or triviate can be used as the vitamin part in the preparation of the complex preparation.

The application of the proposed method significantly increases the yield of transplantable embryos per donor: in cows treated with GSFC, this mask was equal to 2.6 - 2.7. in the group receiving the combined drug - 4.03. This indicates that along with a decrease in the multiplicity and duration of treatments, the yield of fertilized eggs significantly increases, as a result of which the efficiency of the method of transplantation of cattle embryos increases.

Claims (1)

Claims of the method for inducing cows in donor cows during embryo transplantation, including administering a drug based on the follicle-stimulating hormone pituitary gland, in order to reduce the complexity of the method and increase fertility ™, The product additionally contains vitamin A. Vitamin Dz, Vitamin E, 10-th essential solution of lecithin or twin and distilled water with the following ratio of ingredients: Follicle-stimulating hormone of the pituitary gland, mg40 - 50 Vitamin A, ml 24.7-34.6 Vitamin Dz, mg 2.5-3.5 Vitamin E, mg 50-70 10% ester solution of lecithin, 0.5-1.0 or twin drops1-2 Distilled Water 20 or wherein the administration of the drug is carried out once a day for three days. Table 1 Table 2