SU1711734A1 - Method for cultivation of chlorella microalgae - Google Patents

Abstract

The invention relates to the microbiological industry and can be used to obtain microalgae biomass. The purpose of the invention is to increase the yield of the target product. The method involves the cultivation of microalgae in a liquid nutrient medium under the conditions of illumination and mixing under the influence of a pulsed low-frequency magnetic field with a magnetic induction of 2000 Gs at a pulse frequency of 10 Hz and a duration of 10 μs. The method provides an increase in the yield of the target product by 41% in comparison with the control (without the use of a suspension of microalgae), which is 13% more than during cultivation by a known method. 1 dw., 3 tab.

Description

The invention relates to the microbiological industry and can be used in agriculture for the production of microalgae biomass.

Methods are known for producing microalgae biomass on a liquid nutrient medium in tray-type installations, including growing biomass on a liquid nutrient medium in a cultivator installation - virov.

The disadvantage of this method is the low yield of the final product.

Closest to the present invention is a method in which microorganisms are subjected to a pulsed magnetic field with a frequency of 80-120 Hz when the field is induced up to 240 Gs and an exposure time of 1-3 h.

The disadvantage of this method is the insufficiently high biomass yield of microalgae.

The aim of the invention is to increase the yield of the target product.

The proposed method consists in the fact that in the process of cultivation of microalgae on a liquid nutrient medium under conditions of illumination and mixing, the suspension of microalgae is exposed to a pulsed low-frequency field with parameters: pulse frequency 10 Hz, pulse duration 10 µs, magnetic induction 2000 Gs.

The drawing shows an installation diagram for implementing the method.

Single-celled or colonial microalgae, in particular, chlorella from collector 1 through suction pipe 2 by centrifugal electric pump 3 through discharge pipe 4, on which electromagnetic coil 5 with pulse power supply unit 6 is installed, enters the labyrinth tray 7, having a slight slope towards collector 1, exposing pulsed

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magnetic field with the specified parameters.

PRI me R 1. Chlorella is grown under laboratory conditions in culture vessels with a volume of 1 l on a Tamia liquid nutrient medium diluted slightly. Illumination is provided by lamps for 12 hours, the intensity of illumination is 8 klx, the temperature is 28-30 ° C. The suspension is purged with air, after 30 minutes with pure carbon dioxide (200-250 ml / min) for 3 minutes.

In the experimental version, an electromagnetic coil connected to a pulsed power source is installed on a culture vessel. When bubbling the cells fall into the area of impact of a pulsed magnetic field with the parameters: pulse frequency 10 Hz, pulse duration 10 µs, magnetic induction 2000 G, current in the coil 300 A.

In the control, the microalgae were not exposed to a magnetic field.

It has been established (Table 1) that the impact of a low-frequency pulsed magnetic field increases the productivity of chlorella (by the number of cells) by 53–67%.

PRI mme R 2. M and chlorella algae are grown in the labyrinth tray 7 under the open sky when exposed to a suspension by a pulsed electromagnetic field with a pulse frequency of 10 Hz, a pulse duration of 10 µs, and a magnetic induction of 2000 Gs. The installation area is 52.9 m2, the volume of liquid is 10.6 m. The suspension is stirred and subjected to a magnetic field in days

during the 11h light period (from 8 am to 7 pm). Nutrient medium Tamy (twice diluted). Temperature 18 - 29 ° С. Daylight intensity is 82–107 klx. During the daytime, pure carbon dioxide is supplied in an amount of 0.07 - 12, l per m2 / min.

It has been established (Table 2) that the productivity of chlorella when exposed to a low-10-hundredth impulse magnetic field is 20.7 g / m2 acv per day, which is 32% higher compared to the control.

Table 3 shows comparative experimental data on the bio15 mass yield of microalgae chlorella, obtained by the known and proposed method. Cultivation conditions are similar to those shown in Example 2. Increased biomass yield (in g / l asv)

20 by a known method (prototype) averaged 28% against the control (without culture magnetization), and by the proposed method - 41%, i.e. 13% more than the known method of growing

25 microalgae chlorella.

Claims (1)

The invention The method of growing chlorella microalgae in a liquid nutrient medium under conditions of agitation and illumination with 30 impacts on the suspension of microalgae by the electromagnetic field, characterized in that, in order to increase the yield of the target product, the action of the electromagnetic field is carried out by pulsing it with a magnetic induction of 2000 Gs at a pulse frequency of 10 Hz and a duration of 10 µs. The effect of a low-frequency pulsed magnetic field on the productivity of chlorella under laboratory conditions Ј 10 Hz, t - 10 µs B “2000 Gs, I –300 A  Control experience Ј "10 Hz, C - 10" ks B - 2000 Gs, I 300 A Table 1 0.02 0.02 16.2, 0 0.250 0.330 100 153.7 100 W Growing method Continued- Chlorella Density Chlorella Density Productive - Chlorella Growth, Productivity: at the beginning of the experiment at the end of the experiment, g,%,  experience, --.----- dry ve- ---; - 0 /  Som.million Sukhoemln I Dry substances on | By quantity - By dry I ° 2 i ; i niji i ovy 11 i; i "SUTj STW KL6TOK t I (1-L-i. I: i. I: - - -J I cell / ml substance, cell / m / g substance, 1 m, day jcrey cells substance l ........... ,, .., L., i ..: .. J: ....... J ......: ... l ... .......: L ....- .. i. Experience10 f 10 Hz, Ј -GO μs V 2000 Gs, A Control 10 2 i ; i niji i ovy 11 i; i "SUTj STW KL6TOK t I : 1- L -i. i: i. i I cell / ml substance, cell / m / g substance, 1 m, day jcrey cells substance l ........... ,, .., L., i ..: .. J: ....... J ......: ... l ... .......: L ....- .. i. 2.5 0.017 98.0 1, 20.7 148 133 132 with l 2.5 0.01767.0 0.79915.6 100 100 100 Spreadsheets Comparative experimental data on the biomass yield of microalgae chlorella, obtained by various methods The method of growing biomass Control (without magnetization) 0.02 Known method (100 Hz) 0.02 The proposed method is 0.02 g j L am Biomass yield, g / l acv  Source Finite 0.610 0.730 0.360 /